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Structure of the hexagonal surface layer on Caulobacter crescentus…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/s-layer-nmicrobiol201759.pdf6 Apr 2017: Crystals wereflash-frozen in liquid nitrogen for data collection using an additional 25% (vol/vol)PEG 200 as cryo-protectant. ... 200 mesh grids in a Vitrobot Mark IV (FEICompany) maintained at 10 C at 100% relative humidity. -
se060101051p
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/ford01.pdf3 Dec 2004: AP2was dialyzed against 50 mM triethanolamine-KCl,pH 8.0, 200 mM NaCl, 0.2 mM EDTA, 0.1% b-mer-captoethanol, and 0.02% NaN3. -
cde235 2454..2461
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MinC%20EMBO%202001.pdf4 May 2001: 200 column buffer. ... solution, andadditionally 200 mM NaI in the drop. -
� Supplementary Figure 1 Bacterial dynamin-like protein (BDLP) from…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nature%20BDLP%202006%20sup.pdf28 Sep 2006: 50 nm. 100 nm. 200 nm. I. Supplementary Figure 2. The crystal structure of BDLP in GDP-associated and nucleotide-free conformation. -
cm6307.qxd
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/TAXOL.C&B.pdf18 Feb 1999: 110 120 130 140 150 160 170 180 190 200. 210 220 230 240 250 260 270 280 290 300. -
doi:10.1128/mBio.00800-13. 4(6): .mBio. Midcell DNA Translocase FtsK…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/mbio2013bisicchia.pdf23 Dec 2013: 1). An 200-amino-acid N-terminal do-main (FtsKN), which has four or five membrane-spanning seg-. ... C) Sizeexclusion chromatography-multiangle light scattering (SEC-MALS) analysis of TtFtsKN. Using a Superdex S-200 size exclusion column, samples were -
Features critical for membrane binding revealed by DivIVA crystal…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/emboj201099a.pdf20 May 2010: The EMBO Journal, (2010). doi:10.1038/emboj.2010.99 -
Regulation of lipid binding underlies the activation mechanism of…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/22120714.pdf21 Nov 2012: ni. WT/. icW. T/i. 0. 100. 200. 300. 400. CD/n. ic. ... 100. 200. 300. ΔReq. (R. U). 0100200300400500600700800. SH2 deletion lipid kinase dead cancer-linkedmutations:. -
MRC plate poster 18 8 2010 hr
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/PresentationsOther/Poster%20Plates.pdf6 Sep 2010: reservoir holds 200 µl. use robot or manually dispense 0.1 - 10 µl of reservoir and protein. -
1256917combinedsupplement
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Gligoris-SM.pdf14 Nov 2014: loaded onto a 16/600 Superdex 200 gel filtration column (GE Healthcare) in 50 mM Tris pH 7.5, 150 mM NaCl, 1 mM βME. ... Crystals were cryo-protected by adding reservoir solution supplemented with 50-60 % (w/v) PEG 200, before flash freezing in liquid
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