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Protocol for dragonfly copy
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Manuals/Protocol_mosquito_additive.pdf13 Dec 2017: of tips Duration. 1 MRC plate 100 100 0 2 µL 2 16 104 2 min1 MRC plate 200 200 0 5 µL 3.8 31 104 2min 10 sec2 Costar ... plate 200 200 100 5 µL 3.8 31 200 3min 45 sec2 Costar plate 500 500 100 5 µL 7.4 60 200 4min 15 sec. -
TBS may template.qxd
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/epsin/EM/mcmahon.pdf3 May 2002: J. Cell. Biol.155, 193–200. f Takei, K. et al. (1999) Functional partnership betweenamphiphysin and dynamin in clathrin-mediated endocytosis. ... J. Cell. Biol. 155,193–200. 20 Takei, K. et al. (1999) Functional partnershipbetween amphiphysin and -
doi:10.1016/S0076-6879(05)04052-8
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/HigginsMcMahonMethods2005.pdf26 Jan 2006: A lossof 100–200 l is usually observed. Purification of Dynamin and Its Mutants from E. ... Scale bars are 200 nm in. length. [52] IN VITRO RECONSTITUTION OF ENDOCYTIC STAGES 607. -
st7a11.qxd
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MukB%20Structure%201999.pdf10 Sep 1999: mukB V.cholmukB H.inflmukB Y.pestmukB E.coli. S6 H4 H5 H6. ... Protein was eluted with the same buffercontaining 400 mM imidazole. The protein was applied to a sephacrylS-200 column (Amersham-Pharmacia) equilibrated in 20 mM Tris,200 mM NaCl, 1 mM -
PII: S0014-5793(01)02216-5
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MinD%20FEBS.pdf5 Mar 2001: The protein was loaded directly onto a Sephacryl S200column (Amersham-Pharmacia) equilibrated in 200 mM NaCl, 20mM Tris, 1 mM EDTA and 1 mM NaN3 pH 7.0. -
MeganPalm
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/MeganPalm.pdf3 Dec 2005: stops when more than 200 pixels are inside the region. The directional dilation forces. ... 200 eggs from a variety of mutant types and recording conditions illustrate the. -
Phil. Trans. Roy. Soc. Lond. B. 261, 95-104 (1971) ...
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/ptrs71.pdf26 Nov 2012: In some experiments of this sort, using a range 0 to 200 V, the potential was found to vary along the thread proportionately to the electron flux. ... manner. The corresponding interference patterns indicate a n ear-zero potential, a high positive -
mmi_4133.fm
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Spo0J%202004.pdf30 Jun 2004: Spo0J] (mM)0 25 50 100 200 300 25 50 100 200 300. ... The protein was buffer exchanged into20 mM Tris, pH 8.5, 200 mM NaCl using a Sephadex G25 gelfiltration column (Amersham). -
Four-stranded mini microtubules formed by Prosthecobacter BtubAB show …
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2017-Deng-E5950-8.pdf29 Dec 2017: anti-biotin antibody. F 127. catastrophe time. 0. 0.5. 1.0. time (seconds) 200 800. ... of the reaction was pipetted onto freshly glow-dischargedCu/Rh Quantifoil R2/2 200 mesh holey carbon grids (Quantifoil). -
Acetylation of MEK2 and I{kappa}B kinase (IKK) activation loop…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/YopJ.pdf28 Nov 2006: Acetyl Transferase Assays. GST-MEK2 (0.5 g) (purified by usingstandard protocols for GST-tagged protein purification) wasincubated with varying amounts (0 –200 ng) of (His)6-YopJ-wt ... Stimulation of MEK and Erk phosphorylation by various
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