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28 Feb 2023: Threespectra were obtained using an Applied Photophysics Chirascanand a High Precision Cell made of quartz (Hellma Analytics, pathlength 1 mm) between 200 and 250 nm with a bandwidth of 1 ... PROSO II-A new methodfor protein solubility prediction. FEBS J

28 Feb 2023: Thepositive peak near 200 nm, also corresponding to β-sheet, is uncleardue to the access noise. ... g Under HR-TEM, the NSP11-CoV2 fibrils appearunbranched at 192 h; the scale bar represents 200 nm.

28 Feb 2023: The peptide was finally diluted in 20mM sodium phosphate buffer (pH 6.9) with 200 μM ZnCl2 to a finalconcentration of 100 μM. ... The solution was subjected to 5 cyclesof freeze-thaw, after which the preparation of large unilamellar ves-icles was done

28 Feb 2023: gray), 200 (pink), and 100 (purple) μM) in the presence of 10% PEG, was calculated to be an average of 527, 604, and 538 μM, respectively (SI Appendix, Table S1). ... A) Fluorescence images of preformed α-synuclein fibrils (labeled with Alexa Fluor

18 Mar 2023: Fibrils were then diluted to 200 μM,aliquoted and flash frozen in liquid nitrogen, and finally storedat 80 C. ... For the use of kinetic experiments, the 200 μMfibril stock was thawed and sonicated for 15 s using a tip.

28 Feb 2023: Images were collected using aThermo Scientific (FEI) Talos F200X G2 microscope at 200 kV atlow dose using a Ceta 16M CMOS camera.Circular Dichroism.

2 Mar 2023: Initial peptide solutions were prepared by dissolving lyophilizedAβ42 in 6 M GuHCl and purified in 20 mM sodium phosphate buffer,200 μM EDTA, pH 8.0, using a Superdex 75