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  2. Substrate-driven assembly of a translocon for multipass membrane…

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2023/08/Nature_2022b.pdf
    19 Aug 2023: 4c). ΔGapp9.8–0.4–3.7. ΔGapp1. –0.32. –0.33. –3.7. YIPF1(1–200) Sec61β(201–221)N. Flag1 2. YIPF1. ... The 221-residue Flag–YIPF1(1–200)–Sec61β series was generated by Gibson cloning DNA fragments (Twist Biosciences) into a

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