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4 Jun 2020: The far-UV CD spectra of all DesAbs wererecorded from 200 to 250 nm at 20 C, and the spectrum of the buffer wassubtracted from the spectra of all DesAbs. ... Images from 27 different fields of viewwere recorded at 50 ms for 200 frames in each emission

31 Mar 2020: Sequential wash steps with 13 and 21% buffer B(10 mM Tris, 500 mM NaCl, 200 mM imidazole, pH 8.0)over 5 and 7 column volumes each eluted two ... K12CFh was eluted over a lineargradient of 46–200 mM imidazole over 8 column volumes.Fractions were pooled

4 Feb 2020: Moreover, in order to compensatefor the 2.6 times difference in KD, the concentration of αSwas 77 µM and 200 µM when incubated with SUV-0% andSUV-31%, respectively. ... Quartz cuvetteswith path lengths of 1 mm were used, and CD spectra wereobtained by

31 Mar 2020: Expression followed the describedovernight autoinduction steps (42). Recombinant substrate was purifiedover a 46 to 200 mM imidazole gradient over 8 His-trap 5-mL column.

4 Feb 2020: 600. 400. 200. 025Q 97Q-ni 97Q-i. Ave. rage. spo. t ar.