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17 Sep 2017: 20 mM sodium phosphate buffer, pH 8, supplemented with 200 μMEDTA and 0.02% NaN3. ... 0003810002; IKA,Staufen, Germany). Fibrils were diluted to a monomer equivalent concentra-tion of 200 μM, divided into aliquots, flash frozen in liquid N2, and

17 Sep 2017: the binding obtained from the fitting areremarkably different, being more than 200-fold larger for thefibrils than for the monomers.

15 Dec 2017: scale bar, 200 nm).(C) Sedimentation coefficients (S) of thetype-A (orange) and the type-B (green)oligomers measured in arbitrary units.

23 Mar 2017: 70. 150 160 170 180 190 200 210. a c. Heterogenousstates log.

15 Dec 2017: These data, together with 200 nsMD simulations performed on both proteins (SupportingInformation, Figure S12), suggest that the oxetane bridging ofthe disulfide bond does not cause relevant structural modi-fications on the

15 Dec 2017: 200 planes of 1 μm each were acquired per image. For confocal microscopy a Leica SP8 (Leica Microsystems, Wetzlar, Germany) was used at a nominal magnification of 10 or 20X.

19 Jan 2017: A. -30. -25. -20. -15. -10. -5. Wavelength (nm)200 210 220 230 240 250. [ ... μM (violet), 50 μM (pink), 100 μM (blue),150 μM (light blue), and 200 μM (aqua).

22 Jun 2017: on June 22, 2017http://advances.sciencem. ag.org/D. ownloaded from. ranging from 200 to 900 nM (Fig. ... 200 mM EDTA, and 0.02% NaN3, supplemented with 6 mM ThT(13).

17 Sep 2017: For each independent exper-iment at least 200 cells were counted and analyzed for the presence or absence of red puncta. ... incubated at 37 C under constant shaking at 200 rpm for 4 days.

17 Sep 2017: 200 µM EDTA and 0.02% sodium azide (buffer A). The fraction corresponding to the. ... MCT-200-L-C, Union City, CA) and the concentration was determined by absorbance at 280.