Search

16 Feb 2015: triplicate. Size exclusion chro-matography of WT and H50Q fibrils after denaturation andultracentrifugation was performed using a Superdex 200 col-umn on the ÄKTA Explorer apparatus (GE Healthcare). ... PBS. Both the two isoforms show the same pattern

28 Nov 2015: nm) and immersed in water. The systems were energy minimized,then subject to 200 ps of restrained NVT simulation, and furtherequilibrated during 1 ns in the NPT ensemble. ... Thehistone tail values are also much smaller than the measuredpersistence

3 Feb 2015: Igki X5. j 5e j. 4. 200 Ciþ j ð3Þ. where Ci j is the charge of the amino acid i j.Differently from Zyggregator, in Eq. ... Typical-ly, samples contained 10 μM protein in PBS. The far-UVCD spectra of all the variants were recorded from 200 to250 nm at

1 Mar 2015: The coordinates were saved every 20 ps for 200 ns,giving 10000 structures in total.The intensity ratios expected for a purely random coil were. ... After a brief equilibration (200 ps), the coordinates werecollected every 5 ps for 400 ps per replica,

1 Mar 2015: nM; 221 ml for 200 nM) consisting of 50 mM Tris – Maleate(pH 7.0 – 7.4), 10 mg/ml g-globulin, 50 mg/ml

28 Nov 2015: nature structural & molecular biology VOLUME 22 NUMBER 7 JULY 2015 5 5 5. a r t i c l e s. Neurotransmitter release is governed by a sophisticated protein machinery1,2. Central components of this machinery are the SNAREs synaptobrevin, syntaxin-1