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The molecular chaperones DNAJB6 and Hsp70 cooperate to suppress…
www-vendruscolo.ch.cam.ac.uk/aprile2017sr.pdf17 Sep 2017: For each independent exper-iment at least 200 cells were counted and analyzed for the presence or absence of red puncta. ... incubated at 37 C under constant shaking at 200 rpm for 4 days. -
A natural product inhibits the initiation of α-synuclein aggregation…
www-vendruscolo.ch.cam.ac.uk/perni2017pnas-ee.pdf19 Jan 2017: A. -30. -25. -20. -15. -10. -5. Wavelength (nm)200 210 220 230 240 250. [ ... μM (violet), 50 μM (pink), 100 μM (blue),150 μM (light blue), and 200 μM (aqua). -
Physicochemical principles that regulate thecompetition between…
www-vendruscolo.ch.cam.ac.uk/pechmann09pnas.pdf27 Jun 2009: J Mol Biol 200:513–522. 27. Creighton TE (1993) Proteins. Structures and Molecular Properties (Freeman, New York).28. -
Phage display and kinetic selection of antibodies that specifically…
www-vendruscolo.ch.cam.ac.uk/munke2017pnas.pdf17 Sep 2017: 200 µM EDTA and 0.02% sodium azide (buffer A). The fraction corresponding to the. ... MCT-200-L-C, Union City, CA) and the concentration was determined by absorbance at 280. -
Competition between Intramolecular and Intermolecular Interactions in …
www-vendruscolo.ch.cam.ac.uk/routledge09jmb.pdf2 Jun 2009: 784 Competing Interactions in Amyloid Formation. after incubation of the same samples at 200 rpm forN4 weeks at 37 C.In the case of seeded reactions at 37 C, 5% ... The seed consisted of wild-type β2mfibrils, formed in pH2.5 buffer, agitated (200 -
Distinct thermodynamic signatures of oligomer generation in the…
www-vendruscolo.ch.cam.ac.uk/cohen2018nc.pdf22 Apr 2018: 1. 10. Monomer concentration (μM). Am. plitu. de (. a.u. ). 200. a b c. 150. 100. 50. 00 10 20 30 40. ... in 20 mM sodium phosphate buffer, pH 8, with 200 μ M EDTA and 0.02% NaN3. -
NMR characterization of the conformational fluctuations of the human…
www-vendruscolo.ch.cam.ac.uk/leung2014ps.pdf1 Mar 2015: were calculated from Monte Carlo simulations using. 200 randomly distributed synthetic datasets.36 The. ... of 200,000-fold higher affinity. -
Relation between native ensembles and experimentalstructures of…
www-vendruscolo.ch.cam.ac.uk/best06pnas.pdf21 Dec 2006: An ensemble of 200 structures at a temperature of 300K was generated by random superposition of normal modes. -
ar5000117 1..9
www-vendruscolo.ch.cam.ac.uk/obrien2014acr.pdf2 Jun 2014: To use eq 1, three rates must be known at variousnascent chain lengths to make predictions for a specific protein.Although for a 200-residue protein this requirement couldcorrespond to up ... sizes are onaverage between 200 and 300 residues,45 suggesting -
Identification and Structural Characterization of an Intermediate in…
www-vendruscolo.ch.cam.ac.uk/bonetti2016jbc.pdf6 Jun 2016: CD spectra were recorded between 200 and 250 nmusing a quartz cuvette with a light path of 1 cm, at different ureaconcentrations.
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