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Filament structure and subcellular organization of the bacterial…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Liu_PNAS_crescentin_2024.pdf28 Apr 2024: M446 (M443)F. G. 200 Å. Nanobody. Nanobody. Nanobody. Nanobody. Fig. 2. ... Scale bar, 100 nm.) The Insets (Scale bar, 200 nm.) in A and D show a close- up view of the area outlined by the dashed rectangle. -
The patterned assembly and stepwise Vps4-mediated disassembly of…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Hurtig%202023%20sciadv.ade5224.pdf16 Jul 2023: STK strains were supplemented with 200 μM EdU(Thermo Fisher Scientific, C10639) for 10 min. ... Three microliters of resus-pended cells were applied to a freshly glow-discharged QuantifoilAu 200 mesh R2/2 or R2/1 grid. -
CURRICULUM VITAE
https://www2.mrc-lmb.cam.ac.uk/groups/bienz/wp-content/uploads/sites/23/BienzCV_March2024.pdf11 Apr 2024: In "Development and Neurobiology of Drosophila", eds Siddiqui O, Babu P, Hall LM & Hall JC, Plenum Press, New York and London, pp 183-200. -
Substrate-driven assembly of a translocon for multipass membrane…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2023/08/Nature_2022b.pdf19 Aug 2023: 4c). ΔGapp9.8–0.4–3.7. ΔGapp1. –0.32. –0.33. –3.7. YIPF1(1–200) Sec61β(201–221)N. Flag1 2. YIPF1. ... The 221-residue Flag–YIPF1(1–200)–Sec61β series was generated by Gibson cloning DNA fragments (Twist Biosciences) into a -
Diverse cytomotive actins and tubulins share a polymerization switch…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Wagstaff%202023Sci%20Advances%20cytomotive%20switch%20sciadv.adf3021.pdf16 Jul 2023: The exchanged tubulin wasthen injected onto a Superdex 200 3.2/300 column (GE Health-Care), equilibrated, and eluted in 80 mM K-Pipes, 100 μM Na-GDP, 100 μM MgCl2, ... A total of 2.5 μl was appliedto a gold-on-gold grid (UltrAuFoil R2/2 200 mesh),
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