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27 Jan 2018: Briefly, cells were washed three times in PBS and collected by spinning at 200 x g for 5 min. ... The resin was washed five times with 0.1% digitonin, 200 mM NaCl, 50 mM HEPES.

3 Dec 2004: AP2was dialyzed against 50 mM triethanolamine-KCl,pH 8.0, 200 mM NaCl, 0.2 mM EDTA, 0.1% b-mer-captoethanol, and 0.02% NaN3.

17 Jul 2009: 3. Farsad K. et al. J. Cell Biol. 155, 193–200 (2001).4.

4 Aug 2017: 1, A and B). Cross-linking reactionsof the active fractions revealed interacting part-ners of 200, 120, and 100 kD (Fig. ... The gradients were either 2 ml or 0.2 ml, on which either 200 µl or 20 µl of sample were layered.

1 Dec 2010: Smc1DDSmc3AAA. ARS207 ARS231. 150 160 170 180 190 200. 10.0. 5.01.00.0. ... 5.0. 1.00.0. Smc1DD E1158QSmc3AAA. ARS207 ARS231. 150 160 170 180 190 200.

3 Dec 2004: interactions. J. Cell Biol. 155, 193–200 (2001). 20. Zhang, B. et al.

11 Oct 2007: After precipitation in 30% ammonium sulfate saturationand anion-exchange chromatography, the protein wasdialyzed against 50 mM Tris–HCl (pH 8.0), 200 mM KCl,1 mM EDTA, 10% (v/v) glycerol ... Drops were composed of100 nl of protein at 10.0 g/l (with 8

20 Apr 2002: 21 ions. Enzyme(200 ng) was added to the subphase, via an injection port in the Teflontrough, 5 min after the monolayer was spread to allow the surfacepressure to stabilize. ... Replacement of these residues byalanine resulted in a great reduction of the

27 Apr 2015: Protein aggregateswere removed by a second spin at 200,000 g for 10 min. ... 200 pM) was allowed to bind for 10–15 min; this was followed by.

20 Jul 2011: mM Tris pH 7.5, 1 mM EDTA, 1 mM sodium azide and 200 mM NaCl). ... with buffer A being (20 mM Tris pH 8.5, 500 mM NaCl, 1 mM EDTA, 1 mM TCEP) and buffer B (20 mM Tris pH 8.0, 200 mM NaCl,