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17 Sep 2017: 20 mM sodium phosphate buffer, pH 8, supplemented with 200 μMEDTA and 0.02% NaN3. ... 0003810002; IKA,Staufen, Germany). Fibrils were diluted to a monomer equivalent concentra-tion of 200 μM, divided into aliquots, flash frozen in liquid N2, and

17 Sep 2017: the binding obtained from the fitting areremarkably different, being more than 200-fold larger for thefibrils than for the monomers.

15 Dec 2017: scale bar, 200 nm).(C) Sedimentation coefficients (S) of thetype-A (orange) and the type-B (green)oligomers measured in arbitrary units.

23 Mar 2017: 70. 150 160 170 180 190 200 210. a c. Heterogenousstates log.

15 Dec 2017: These data, together with 200 nsMD simulations performed on both proteins (SupportingInformation, Figure S12), suggest that the oxetane bridging ofthe disulfide bond does not cause relevant structural modi-fications on the

15 Dec 2017: 200 planes of 1 μm each were acquired per image. For confocal microscopy a Leica SP8 (Leica Microsystems, Wetzlar, Germany) was used at a nominal magnification of 10 or 20X.

19 Jan 2017: A. -30. -25. -20. -15. -10. -5. Wavelength (nm)200 210 220 230 240 250. [ ... μM (violet), 50 μM (pink), 100 μM (blue),150 μM (light blue), and 200 μM (aqua).

22 Jun 2017: on June 22, 2017http://advances.sciencem. ag.org/D. ownloaded from. ranging from 200 to 900 nM (Fig. ... 200 mM EDTA, and 0.02% NaN3, supplemented with 6 mM ThT(13).

17 Sep 2017: For each independent exper-iment at least 200 cells were counted and analyzed for the presence or absence of red puncta. ... incubated at 37 C under constant shaking at 200 rpm for 4 days.

17 Sep 2017: 200 µM EDTA and 0.02% sodium azide (buffer A). The fraction corresponding to the. ... MCT-200-L-C, Union City, CA) and the concentration was determined by absorbance at 280.

17 Sep 2017: Origin of. the CID. domain. 600 500 400 300 200 100 Present time. ... 1.5 104. -1 104. -5000. 0. 5000. 200 210 220 230 240 250 260.

13 Apr 2017: was composed of: 12 mM OPA, 18 mM BME, and 4% w/v SDS in 200 mM carbonate buffer, pH. ... 17. 200 µL, depending on the nature of the particular experiment, though even smaller volumes can be.

17 Sep 2017: The aggregation of -Syn (200 M) was made at pH 7.4 and 37 C in the presence of an equal molarconcentration of MOAG-4. ... Binding of MOAG-4 to ThT was negligible. SDS-PAGE analysis. A 250-l volume of 200 M -Syn in 50 mM Tris-HCl, 150mM NaCl, and 3 mM

17 Sep 2017: The pellets were resuspended and incubated(4 C for 2 h) in 25 mL of solubilization buffer [200 mM NaCl,20 mM imidazole, 10 mM β-mercaptoethanol, 40 mM DDM,and

17 Sep 2017: phosphate,pH 7.5, 100 mM NaCl, 2 mM EDTA, and 0.1% NaN3)were incubated for a period of 107 h at 37 C with con-stant agitation at 200

17 Sep 2017: For correspondence: durocher@. lunenfeld.ca (DD); kay@pound. med.utoronto.ca (LEK). †These authors contributed. equally to this work‡These authors also contributed. equally to this work. Present address: The Francis. Crick Research Institute,