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1 Mar 2015: 12.). 200 nm. Figure 1. Amyloid fibrils formed from peptide fragments of transthyretin, a protein associated withseveral misfolding diseases.

21 Feb 2015: 3.5 200 µV. 100 nM kainate (control)ba. 50 nM monomeric Aβ42100 nM kainate. ... gel filtration on a 3.4 cm 200 cm gel-filtration column at 4 C.

10 Feb 2015: 3. 0. 4. 2. 6. 8. 10. 12. 14. 190 200 210 220 230 240 2500 5 10 15 20. ... 0. 200. 400. 600. 800. 1,000. 0 10 20 30 40 50.

28 Nov 2015: F. B 0. MER. 200 220 240wavelength (nm). -10000. MER. MRE WavelengthTemperature (C). ... mn. (ht. gnel. eva. W202. 200. 198. 196. 0 20 40 60 80 100.

10 Aug 2015: The far-UV CD spectra of all the variants wererecorded from 200 to 250 nm at 25 C, and the spectrum of thebuffer was systematically subtracted from the spectra of allprotein ... or presence of a 1:200(Hsp70:Aβ42) molar ratio of the chaperone variants.

10 Aug 2015: We found that the median number is 200, and themean is 570 (Fig.

1 Mar 2015: were calculated from Monte Carlo simulations using. 200 randomly distributed synthetic datasets.36 The. ... of 200,000-fold higher affinity.

28 Nov 2015: Aβ40 was thenadded (2535 μL in 10 mM NaOH) to give a final buffercomposition identical to that of the isotropic samples and afinal volume of 200 μL. ... RDCs at an ionic strength of 200 mM and found thatsystematic errors were reduced (Q = 0.13 with

10 Feb 2015: Protein concentrations were in the range of 200–350 μM. For the measurements of the bound state, N,N′,N"-triacetylchitotriose (triNAG) sugar was purchased from Sigma and dissolved in water ... Serial dilutions (200 µM, 100 µM, 50 µM, 25 µM, and

3 Feb 2015: Thesystem was then evolved for further 200 ps at constanttemperature. After that, KΘ was further increased linearly from5000 to 15000 kJ/mol in 200 ps.

16 Feb 2015: triplicate. Size exclusion chro-matography of WT and H50Q fibrils after denaturation andultracentrifugation was performed using a Superdex 200 col-umn on the ÄKTA Explorer apparatus (GE Healthcare). ... PBS. Both the two isoforms show the same pattern

28 Nov 2015: nm) and immersed in water. The systems were energy minimized,then subject to 200 ps of restrained NVT simulation, and furtherequilibrated during 1 ns in the NPT ensemble. ... Thehistone tail values are also much smaller than the measuredpersistence

3 Feb 2015: Igki X5. j 5e j. 4. 200 Ciþ j ð3Þ. where Ci j is the charge of the amino acid i j.Differently from Zyggregator, in Eq. ... Typical-ly, samples contained 10 μM protein in PBS. The far-UVCD spectra of all the variants were recorded from 200 to250 nm at

1 Mar 2015: The coordinates were saved every 20 ps for 200 ns,giving 10000 structures in total.The intensity ratios expected for a purely random coil were. ... After a brief equilibration (200 ps), the coordinates werecollected every 5 ps for 400 ps per replica,

1 Mar 2015: nM; 221 ml for 200 nM) consisting of 50 mM Tris – Maleate(pH 7.0 – 7.4), 10 mg/ml g-globulin, 50 mg/ml

28 Nov 2015: nature structural & molecular biology VOLUME 22 NUMBER 7 JULY 2015 5 5 5. a r t i c l e s. Neurotransmitter release is governed by a sophisticated protein machinery1,2. Central components of this machinery are the SNAREs synaptobrevin, syntaxin-1