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Competition between Folding, Native-State Dimerisation and Amyloid…
www-vendruscolo.ch.cam.ac.uk/hamada09jmb.pdf8 Jul 2009: Thepolyvinyl formal–carbon-coated 200-mesh copper grids(200 Å) were obtained from Oken Shoji (Tokyo, Japan). -
1 Published in final edited form as: Nature Chemistry ...
www-vendruscolo.ch.cam.ac.uk/NatChem_AuthorAccepted_100417.pdf13 Apr 2017: was composed of: 12 mM OPA, 18 mM BME, and 4% w/v SDS in 200 mM carbonate buffer, pH. ... 17. 200 µL, depending on the nature of the particular experiment, though even smaller volumes can be. -
The H50Q Mutation Induces a 10-fold Decrease in theSolubility ...
www-vendruscolo.ch.cam.ac.uk/porcari2015jbc.pdf16 Feb 2015: triplicate. Size exclusion chro-matography of WT and H50Q fibrils after denaturation andultracentrifugation was performed using a Superdex 200 col-umn on the ÄKTA Explorer apparatus (GE Healthcare). ... PBS. Both the two isoforms show the same pattern -
Structure-Based Discovery of Small-Molecule Inhibitors of…
www-vendruscolo.ch.cam.ac.uk/Chia-2023-MP.pdf18 Mar 2023: Fibrils were then diluted to 200 μM,aliquoted and flash frozen in liquid nitrogen, and finally storedat 80 C. ... For the use of kinetic experiments, the 200 μMfibril stock was thawed and sonicated for 15 s using a tip. -
THE JOURNAL OF CHEMICAL PHYSICS 135, 065107 (2011) Nucleated ...
www-vendruscolo.ch.cam.ac.uk/cohen11jcp3.pdf15 Jan 2012: 200 400 600 800 10000.000. 0.002. 0.004. 0.006. 0.008. 0.010. 0.012. -
Amyloid-like Fibrils from an α-Helical Transmembrane Protein
www-vendruscolo.ch.cam.ac.uk/stroobants2017b.pdf17 Sep 2017: The pellets were resuspended and incubated(4 C for 2 h) in 25 mL of solubilization buffer [200 mM NaCl,20 mM imidazole, 10 mM β-mercaptoethanol, 40 mM DDM,and -
THE JOURNAL OF CHEMICAL PHYSICS 139, 034101 (2013) A ...
www-vendruscolo.ch.cam.ac.uk/suardiaz2013jcp.pdf16 Jul 2013: RMSD (ppm) in training data set 0.200 0.295 0.169 0.396 0.175RMSD (ppm) in validation data set 0.188 0.305 0.166 0.386 0.179. -
research 1..8
www-vendruscolo.ch.cam.ac.uk/roodveldt12b.pdf31 Oct 2012: SH-SY5Y cells were cultured in 96-well plates in 200 μL of complete DMEM-F12 medium andallowed to undergo a maximum of 20 passes for all cytotoxicityassays. -
jp3057306 1..10
www-vendruscolo.ch.cam.ac.uk/sahakyan13jpcb.pdf3 Mar 2013: Analysis of the Contributions of Ring Current and Electric FieldEffects to the Chemical Shifts of RNA BasesAleksandr B. Sahakyan and Michele Vendruscolo. Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K. S -
Mapping of Two Networks of Residues That Exhibit Structural and…
www-vendruscolo.ch.cam.ac.uk/dhulesia08jacs.pdf8 Jul 2009: factor between angle distributions made out of 11 200 and 160structures (this corresponds to different time intervals between theextraction of structures during the dynamics). ... e 18. , 200. 8 on. http. ://pu. bs.a. cs.o. rg |. doi:. 10.1. 021/. ja07.
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