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16 Jul 2023: STK strains were supplemented with 200 μM EdU(Thermo Fisher Scientific, C10639) for 10 min. ... Three microliters of resus-pended cells were applied to a freshly glow-discharged QuantifoilAu 200 mesh R2/2 or R2/1 grid.

3 Dec 2004: AP2was dialyzed against 50 mM triethanolamine-KCl,pH 8.0, 200 mM NaCl, 0.2 mM EDTA, 0.1% b-mer-captoethanol, and 0.02% NaN3.

17 Jul 2009: 3. Farsad K. et al. J. Cell Biol. 155, 193–200 (2001).4.

4 Aug 2017: 1, A and B). Cross-linking reactionsof the active fractions revealed interacting part-ners of 200, 120, and 100 kD (Fig. ... The gradients were either 2 ml or 0.2 ml, on which either 200 µl or 20 µl of sample were layered.

1 Dec 2010: Smc1DDSmc3AAA. ARS207 ARS231. 150 160 170 180 190 200. 10.0. 5.01.00.0. ... 5.0. 1.00.0. Smc1DD E1158QSmc3AAA. ARS207 ARS231. 150 160 170 180 190 200.

3 Dec 2004: interactions. J. Cell Biol. 155, 193–200 (2001). 20. Zhang, B. et al.

20 Apr 2002: 21 ions. Enzyme(200 ng) was added to the subphase, via an injection port in the Teflontrough, 5 min after the monolayer was spread to allow the surfacepressure to stabilize. ... Replacement of these residues byalanine resulted in a great reduction of the

11 Oct 2007: After precipitation in 30% ammonium sulfate saturationand anion-exchange chromatography, the protein wasdialyzed against 50 mM Tris–HCl (pH 8.0), 200 mM KCl,1 mM EDTA, 10% (v/v) glycerol ... Drops were composed of100 nl of protein at 10.0 g/l (with 8

27 Apr 2015: Protein aggregateswere removed by a second spin at 200,000 g for 10 min. ... 200 pM) was allowed to bind for 10–15 min; this was followed by.

20 Jul 2011: mM Tris pH 7.5, 1 mM EDTA, 1 mM sodium azide and 200 mM NaCl). ... with buffer A being (20 mM Tris pH 8.5, 500 mM NaCl, 1 mM EDTA, 1 mM TCEP) and buffer B (20 mM Tris pH 8.0, 200 mM NaCl,

17 Jan 2006: Bars, 200 nm. 4535Sorting Nexin-2 and CI-MPR retrieval. mammalian orthologue of the yeast retromer componentVps17p (Haft et al., 2000). ... J. Cell Biol. 155, 193-200. Gagescu, R., Gruenberg, J. and Smythe, E.

17 Mar 2003: O.B., Jr., Krupnick, J.G., Vsevolod, V., Gurevich, V.V.,solution (30% PEG MME 5000, 200 mM (NH4)2SO4, 100 mM MESBenovic, J.L., and Keen, J.H. ... Hao, W., Tan, Z., Prasad, K., Reddy, K.K., Chen, J., Prestwich, G.D.,vent consisting of 40% PEG 400, 200 mM

11 Apr 2024: In "Development and Neurobiology of Drosophila", eds Siddiqui O, Babu P, Hall LM & Hall JC, Plenum Press, New York and London, pp 183-200.

20 Apr 1999: Twenty four micrographs out of 200 in the tilt range of 0–60 wereselected and scanned on a Zeiss SCAI scanner with a 28µm step size.Images were processed with the

16 Aug 2008: tion plates mixing 200 200 nl of protein and crystallization solution. ... KOPS1 DNA. Crystallization solution was 200 mM citric acid/NaOH pH 3.5.

30 Apr 1999: 2,000. 500. a. Time (min). Time (min). 200. µM GTP. GT. ... ferase-tagged amphiphysin-2 SH3 domain on glutathione–agarose beads at 4 C. After extensive washing of the matrix in buffer A (200 mM NaCl, 20 mM HEPES, pH 7.3,

6 Oct 2004: indicated dishes were treated with 200 nM wortmannin 40 minRap on ARAP3 Rho GAP activity (Figure 3C).

30 Apr 2010: 4. 100 mM CaCl2: Dissolve in water. Keep at 4C.5. 200 mM EGTA. ... A good range to start is between 30and 200 µL of packed bead volume.

25 Nov 2019: Nature Structural & Molecular Biology, doi:10.1038/s41594-019-0331-x

9 May 2003: concentration of approximately 200 nM. Combined with the lack of putative voltage gated Na+ channels in the C.elegans. ... change, corresponding to an apparent free calcium concentration of approx. 200 nM.