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Structures of endocytic proteins
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/research/structur/structure.html27 Jun 2007: Gallery of structure for endocytic proteins. There are also links to pages for many endocytic and trafficking proteins. -
Liposome and Lipid tubule Production
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/techniqs/Liposome.html25 Jun 2007: NB: the filtration process usually leads to loss of around 100-200 ul of liquid, and 10-20% of the lipid in the reamining volume. -
Negative-staining protocol for EM: Harvey McMahon lab
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/techniqs/Negative%20stain%20protocol.html25 Jun 2007: proteins, or complexes smaller than 200 kDÑin my hands, GroEL 14mers (840kD) or clathrin triskelia (600 kD) can be made out on a good grid but HSP60 monomers cannot). -
DOI: 10.1126/science.1136985 , 120 (2007); 316Science et al.Maria C.…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Alonso_Science.pdf6 Apr 2007: on. Apr. il 6,. 200. 7 w. ww. sci. ence. mag. ... REPORTS. on. Apr. il 6,. 200. 7 w. ww. sci. ence. -
Alonsoetal Supplementary Material 030107
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Alonso-suppmat.pdf2 Apr 2007: buffer at 200 V for 1.5 hours. -
ESMS of membrane proteins
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/methods/ESMS%20of%20membrane%20proteins.pdf20 Jul 2007: mix - add 200 μl water, mix vigorously - centrifuge 2 min 10.000x g - take top phase off ( 500 μl), protein forms precipitated layer - add 300 μl methanol - centrifuged 1 min -
doi:10.1016/j.str.2007.05.002
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/FCH02F-BARHenne07.pdf2 Jun 2007: a Superdex 200 column; 130 mM mFCHo2 (res-. idues 1–261) was loaded. ... H) Control Folch fraction 1 liposomes without addition of protein. The scale bars represent 200 nm. -
doi:10.1016/j.jmb.2007.08.056
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Oliva_FtsZ_JMB_2007.pdf11 Oct 2007: After precipitation in 30% ammonium sulfate saturationand anion-exchange chromatography, the protein wasdialyzed against 50 mM Tris–HCl (pH 8.0), 200 mM KCl,1 mM EDTA, 10% (v/v) glycerol ... Drops were composed of100 nl of protein at 10.0 g/l (with 8 -
Structural insight into the ESCRT-I/-II link and itsrole in ...
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17215868.pdf14 Jun 2007: 0 50 100 150 200. 0.22. 0.27 27 nM. 61 nM. ... Superdex 200 10/300 column. 16 20128. 6 10 14 18Elution volume (ml). -
DOI: 10.1126/science.1135394 , 239 (2007); 317Science et al.Nabil…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17626883.pdf8 Dec 2007: 150. 200. WT E542K E545K Q546K- - - - p85ni. p110 α. ... 0. 50. 100. 150. 200. 250. - - Phosphopeptide. C. B.
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