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17 Mar 2004: J. Cell Biol. 155,193-200.

19 Nov 2004: sZ-N. TmFt. sZ-C. TmFt. sZ-N. C. TmFtsZ TmFtsZ-N. TmFtsZ-C TmFtsZ-NC. Wavelength (nm)200 210 220 230 240 250 260. ... 081% (w/v) malachite green and 2 ml of water were mixed and 200 µl was added to 50-µl reactions contain-ing proteins or inorganic

30 Jun 2004: Spo0J] (mM)0 25 50 100 200 300 25 50 100 200 300. ... The protein was buffer exchanged into20 mM Tris, pH 8.5, 200 mM NaCl using a Sephadex G25 gelfiltration column (Amersham).

3 Dec 2004: interactions. J. Cell Biol. 155, 193–200 (2001). 20. Zhang, B. et al.

6 Oct 2004: indicated dishes were treated with 200 nM wortmannin 40 minRap on ARAP3 Rho GAP activity (Figure 3C).

3 Dec 2004: AP2was dialyzed against 50 mM triethanolamine-KCl,pH 8.0, 200 mM NaCl, 0.2 mM EDTA, 0.1% b-mer-captoethanol, and 0.02% NaN3.

3 Dec 2004: AP2was dialyzed against 50 mM triethanolamine-KCl,pH 8.0, 200 mM NaCl, 0.2 mM EDTA, 0.1% b-mer-captoethanol, and 0.02% NaN3.

3 Dec 2004: interactions. J. Cell Biol. 155, 193–200 (2001). 20. Zhang, B. et al.

3 Dec 2004: AP2was dialyzed against 50 mM triethanolamine-KCl,pH 8.0, 200 mM NaCl, 0.2 mM EDTA, 0.1% b-mer-captoethanol, and 0.02% NaN3.

23 Nov 2004: 2H2O buffer containing 20mM sodium phosphate ( pH 6.5), 200 mM NaCl, 2 mM2H10 dithiothreitol, and 0.01% sodium azide.

16 Aug 2004: Tris (pH 7.5),200 mM NaCl and 5 mM MgCl2 and eluted separately.Following calibration, 10 ml of ZapA protein at 10 mg/ml was injected and eluted as a ... 1 mM EDTA,1 mM NaN3 and 200 mM NaCl was placed in thesample compartment of an Epon double-sector

23 Nov 2004: Cells were incubated A series of frames depicting a GFP-SNX1-decorated tubule trans-with either 200 ng/ml TxR-EGF (A) or 20 g/ml Alexa568-transferrin (B) porting ... The scale barrepresents 200 nm. pressed at high levels, membrane association of wild-

15 Apr 2004: Calcu-lations employed torsional dynamics at 50 000 K for 1000steps of 15 ps, then a first slow cooling stage of torsionaldynamics (200 cycles each of 1000 steps of 15

29 Dec 2004: Since K-252a is a much more potent kinase inhibitor than H-9, and some non- specific effects were noted at concentrations of H-9 in excess of 200 pM, most ... Aliquots of cpt-CAMP-treated cells were further treated for 10 min with K-252a (20 PM) or H-9

4 May 2004: 510 100 1;0 200 2;0 310 3;0 400 4 ; 0 500.

21 Dec 2004: Unless specifically noted otherwise, transfections with Lipo-fectamine 2000 (Invitrogen, Carlsbad, CA) were performed in 96-well plateswith a total of 200 ng of plasmid DNA per well. ... Of this 200 ng, the luciferasereporter plasmid was always kept

1 Nov 2004: on Superdex 200 16/60 equilibrated in buffer C. ... A 200–250 g aliquot of GST-tagged pro-taining 3.1% PEG 35000, 0.1 M Tris acetate (pH 8.5), 1.36 M sodiumtein and 600–700 g of

8 Nov 2004: ng TF ng PrP-TF. 5 10 200 Opn. PrP. Prl. ng TF 10 ng SS-TF. ... 200 ngDNA per well.

23 Nov 2004: N-BAR SH3. GTPase PH GED. PTB. ATPase. 200 amino acids. EH EH. ... 1.9(1.1) No bindingEpsin1-P1 APAFSDPWGGSP 200(1.2).

15 Jan 2004: Samples were treatedwith PI (200 g of aprotinin and leupeptin/ml and 5 mM phenylmethylsulfonylfluoride), transferred to five volumes of preheated PK inactivation buffer (1%sodium dodecyl sulfate [SDS], 0.1 ... E to H) Brain homogenates from