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30 Sep 2009: I C. ON. SOR. TIA. UK. on. July. 16,. 200. 9Pu. ... blis. hed. on M. ay 8. , 200. 9 on. http.

30 Sep 2009: 200. 9 | d. oi: 1. 0.10. 21/ja. 9037. 72t. almost9 (available for download at http://open-almost.org), inaccordance with the CHARMM22 topology file.18 We left out mostdistances ... epte. mbe. r 9,. 200. 9 | d. oi: 1. 0.10.

30 Sep 2009: 200. 8 N. atur. e P. ublis. hing. Gro. up h.

8 Jul 2009: 009. Publ. ishe. d on. Mar. ch 3. , 200. 9 on. ... ch 3. , 200. 9 on. http. ://pu. bs.a. cs.o. rg |. doi:. 10.1. 021/. ja80. 8729. 5.

11 Apr 2012: Mapping Long-Range Interactions in r-Synuclein using Spin-Label NMR andEnsemble Molecular Dynamics Simulations. Matthew M. Dedmon, Kresten Lindorff-Larsen, John Christodoulou, Michele Vendruscolo, andChristopher M. Dobson. Department of Chemistry,

4 Jul 2016: N(F1)), HNCO (1666:H/40:C/72:N), and HNHA(1666:H/200:HA/72:N). ... the alignment medium used didnot significantly perturb the conformational ensemble of α-synuclein.RDCs were measured for samples of α-synuclein (200 μM).

23 Jan 2016: evia. tion. (Å). a6. 5. 4. 3. 2. 1. 0 100 200 300. ... m.s. dev. iatio. n (Å. )c. Time (ns)0 100 200 300.

16 Jan 2011: score28 explaining the very good decoy distribution. The larger proteins of about 200 amino acids have. ... such that the largest cluster contained 500 instead of 200. structures brought no significant changes.

17 Sep 2017: 20 mM sodium phosphate buffer, pH 8, supplemented with 200 μMEDTA and 0.02% NaN3. ... 0003810002; IKA,Staufen, Germany). Fibrils were diluted to a monomer equivalent concentra-tion of 200 μM, divided into aliquots, flash frozen in liquid N2, and

22 Jun 2017: on June 22, 2017http://advances.sciencem. ag.org/D. ownloaded from. ranging from 200 to 900 nM (Fig. ... 200 mM EDTA, and 0.02% NaN3, supplemented with 6 mM ThT(13).

19 Jan 2017: A. -30. -25. -20. -15. -10. -5. Wavelength (nm)200 210 220 230 240 250. [ ... μM (violet), 50 μM (pink), 100 μM (blue),150 μM (light blue), and 200 μM (aqua).

17 Sep 2017: For each independent exper-iment at least 200 cells were counted and analyzed for the presence or absence of red puncta. ... incubated at 37 C under constant shaking at 200 rpm for 4 days.

17 Sep 2017: 200 µM EDTA and 0.02% sodium azide (buffer A). The fraction corresponding to the. ... MCT-200-L-C, Union City, CA) and the concentration was determined by absorbance at 280.

27 Jun 2009: J Mol Biol 200:513–522. 27. Creighton TE (1993) Proteins. Structures and Molecular Properties (Freeman, New York).28.

2 Jun 2009: 784 Competing Interactions in Amyloid Formation. after incubation of the same samples at 200 rpm forN4 weeks at 37 C.In the case of seeded reactions at 37 C, 5% ... The seed consisted of wild-type β2mfibrils, formed in pH2.5 buffer, agitated (200

22 Apr 2018: 1. 10. Monomer concentration (μM). Am. plitu. de (. a.u. ). 200. a b c. 150. 100. 50. 00 10 20 30 40. ... in 20 mM sodium phosphate buffer, pH 8, with 200 μ M EDTA and 0.02% NaN3.

1 Mar 2015: were calculated from Monte Carlo simulations using. 200 randomly distributed synthetic datasets.36 The. ... of 200,000-fold higher affinity.

21 Dec 2006: An ensemble of 200 structures at a temperature of 300K was generated by random superposition of normal modes.

2 Jun 2014: To use eq 1, three rates must be known at variousnascent chain lengths to make predictions for a specific protein.Although for a 200-residue protein this requirement couldcorrespond to up ... sizes are onaverage between 200 and 300 residues,45 suggesting

6 Jun 2016: CD spectra were recorded between 200 and 250 nmusing a quartz cuvette with a light path of 1 cm, at different ureaconcentrations.

4 Feb 2020: Moreover, in order to compensatefor the 2.6 times difference in KD, the concentration of αSwas 77 µM and 200 µM when incubated with SUV-0% andSUV-31%, respectively. ... Quartz cuvetteswith path lengths of 1 mm were used, and CD spectra wereobtained by

18 Dec 2018: umbe. rs). 150250. 200. 150. 100. 50. 0. 200. 150. 100. ... umbe. rs). 800. 700. 600. 500. 400. 300. 200. 100. 0.

3 Jan 2007: equilibrating at 300 K for 200 ps, the simulation was carriedout for 4 ns and in the constant volume, isothermal (NVT) canonicalensemble via the Nose-Hoover temperature coupling scheme. ... However, the structures determined using RDC refinementemployed

15 Jan 2012: THE JOURNAL OF CHEMICAL PHYSICS 135, 065106 (2011). Nucleated polymerization with secondary pathways. II. Determinationof self-consistent solutions to growth processes described by non-linearmaster equations. Samuel I. A. Cohen, Michele Vendruscolo,

28 Nov 2015: Aβ40 was thenadded (2535 μL in 10 mM NaOH) to give a final buffercomposition identical to that of the isotropic samples and afinal volume of 200 μL. ... RDCs at an ionic strength of 200 mM and found thatsystematic errors were reduced (Q = 0.13 with

12 Aug 2014: In thefirst step a 50-ps simulation was performed by keeping the heavyatoms of the protein fixed; then a second 200-ps simulation wasperformed without any restraint. ... The density of the system wasrelaxed by a 200-ps run using the Berendsen barostat (14

16 Jul 2013: 298.15. 350. 475. 0 25 50 125 150 200 3000. 500.

17 Sep 2017: the binding obtained from the fitting areremarkably different, being more than 200-fold larger for thefibrils than for the monomers.

17 Sep 2017: Origin of. the CID. domain. 600 500 400 300 200 100 Present time. ... 1.5 104. -1 104. -5000. 0. 5000. 200 210 220 230 240 250 260.

13 Jan 2010: Nat. Rev.,Genet. 7, 200–210. 51. Wilkinson, D. L. & Harrison, R.

27 Jun 2009: e. 200 of the 799 that were available (see Table1). In this case we found the structure of minimal overall scoreat 3.47 Å CR rmsd.

17 Sep 2017: The aggregation of -Syn (200 M) was made at pH 7.4 and 37 C in the presence of an equal molarconcentration of MOAG-4. ... Binding of MOAG-4 to ThT was negligible. SDS-PAGE analysis. A 250-l volume of 200 M -Syn in 50 mM Tris-HCl, 150mM NaCl, and 3 mM

24 Nov 2007: Scale bar 200 nm for both panels.Found at doi:10.1371/journal.pbio.0050290.sg003 (2.3 MB TIF).

10 Feb 2015: Protein concentrations were in the range of 200–350 μM. For the measurements of the bound state, N,N′,N"-triacetylchitotriose (triNAG) sugar was purchased from Sigma and dissolved in water ... Serial dilutions (200 µM, 100 µM, 50 µM, 25 µM, and

8 Jul 2009: ThT assay. Aliquots (300 μl) of each sample were withdrawn atregular time intervals and mixed with 200 μl of 50 mMacetate buffer, pH 5.5, 25 C, containing 110 μM ThT.

23 Sep 2018: The. protein was eluted with a linear gradient of 0–1M. NaCl and subsequently purified on a Superdex 200. ... 12 h at 308C. To insure complete conversion to phos-. phorylated state, 500 lg of 15N a-synuclein wereincubated in 200 lL of reaction volume.

4 Jul 2016: 0. 1. 2. 3. 4. 5. 6. 7. 8. 0 100 200 300 400 500 6000. ... 2. 4. 6. 7. 8. 0 100 200 300 400 500 600Serial number of sorted NOE restraints.

3 Feb 2015: Thesystem was then evolved for further 200 ps at constanttemperature. After that, KΘ was further increased linearly from5000 to 15000 kJ/mol in 200 ps.

15 Jan 2012: 2). The free energy maps have been generatedfrom 200,000 conformations in each sampling.

13 Jan 2010: A. (1988). J. Mol. Biol. 200, 513 – 522.47. Cowan, R. &

8 Jul 2009: Thepolyvinyl formal–carbon-coated 200-mesh copper grids(200 Å) were obtained from Oken Shoji (Tokyo, Japan).

13 Apr 2017: was composed of: 12 mM OPA, 18 mM BME, and 4% w/v SDS in 200 mM carbonate buffer, pH. ... 17. 200 µL, depending on the nature of the particular experiment, though even smaller volumes can be.

16 Feb 2015: triplicate. Size exclusion chro-matography of WT and H50Q fibrils after denaturation andultracentrifugation was performed using a Superdex 200 col-umn on the ÄKTA Explorer apparatus (GE Healthcare). ... PBS. Both the two isoforms show the same pattern

18 Mar 2023: Fibrils were then diluted to 200 μM,aliquoted and flash frozen in liquid nitrogen, and finally storedat 80 C. ... For the use of kinetic experiments, the 200 μMfibril stock was thawed and sonicated for 15 s using a tip.

15 Jan 2012: 200 400 600 800 10000.000. 0.002. 0.004. 0.006. 0.008. 0.010. 0.012.

17 Sep 2017: The pellets were resuspended and incubated(4 C for 2 h) in 25 mL of solubilization buffer [200 mM NaCl,20 mM imidazole, 10 mM β-mercaptoethanol, 40 mM DDM,and

16 Jul 2013: RMSD (ppm) in training data set 0.200 0.295 0.169 0.396 0.175RMSD (ppm) in validation data set 0.188 0.305 0.166 0.386 0.179.

31 Oct 2012: SH-SY5Y cells were cultured in 96-well plates in 200 μL of complete DMEM-F12 medium andallowed to undergo a maximum of 20 passes for all cytotoxicityassays.

3 Mar 2013: Analysis of the Contributions of Ring Current and Electric FieldEffects to the Chemical Shifts of RNA BasesAleksandr B. Sahakyan and Michele Vendruscolo. Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K. S

8 Jul 2009: factor between angle distributions made out of 11 200 and 160structures (this corresponds to different time intervals between theextraction of structures during the dynamics). ... e 18. , 200. 8 on. http. ://pu. bs.a. cs.o. rg |. doi:. 10.1. 021/. ja07.