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  2. Supplementary Figure 1Lysophosphatidic acid acyl transferase (LPAAT)…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndoSupFigs.pdf
    4 Jan 2006: fter. th. rom. bin cl. eava. ge o. f GST. ). 0.01. 0.1. 1. 10. 0 100 200 300 400. ln OD600. ... 1. 10. 0 100 200 300 400. ln OD600. pPLSC (E.coli LPAAT).
  3. NPGRJ_NSMB_1158 1..8

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nsmbFtsK2006.pdf
    12 Oct 2006: c) Superdex 200 gel filtration of E. coli FtsKg in the presence of DNA. ... Storage buffer was. 20 mM Tris-HCl, 200 mM NaCl, 1 mM EDTA and 1 mM sodium azide.
  4. Optimization of crystallization conditions with additive screening…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/PresentationsOther/additive-screening.pdf
    9 Jan 2017: 200 nl protein 200 nl condition 100 nl additive). This late approach facilitates the screening at different concentrations of additives (by simply varying the volume of additive screen added) and implies
  5. Cryo‐EM structure of the full‐length Lon protease from Thermus…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Lon%20Coscia%202021%20FEBS.pdf
    14 Nov 2021: min). Quantifoil Cu/Rh 200 mesh R2/2 grids. The same Lon. sample, diluted 10 times in buffer A (Lon concentration. ... Tris/HCl pH8.0, 200 mM NaCl, 10 mM MgCl2, 5 mM ATP.
  6. Alonsoetal Supplementary Material 030107

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Alonso-suppmat.pdf
    2 Apr 2007: buffer at 200 V for 1.5 hours.
  7. Proteins containing photosynthetic reaction centre domains modulate…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Nussbaum_Nat_micro_2024.pdf
    1 Mar 2024: CdpB2. SepF. SepFCdpB1. 200. Abso. rban. ce (a. u.). Abso. rban. ... For 1 transformation, 200 µl of the prepared cell suspension was used.
  8. DOI: 10.1126/science.1165322 , 1710 (2008); 322Science et al.Rachel…

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/19008417.pdf
    18 Dec 2008: on. Dec. embe. r 18. , 200. 8 w. ww. sci. ... embe. r 18. , 200. 8 w. ww. sci. ence. mag.
  9. The structure of human thyroglobulin

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/TG_Nature2020.pdf
    31 Jan 2020: Superose 6 Increase 3.2/300 column (GE Healthcare), equilibrated with buffer T200 (50 mM Tris-HCl, 200 mM NaCl, pH 8.0). ... equili-brated with 20 mM Hepes, 200 mM NaCl at pH 7.5.
  10. IL-17 is a neuromodulator of Caenorhabditis elegans sensory responses

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Nature_2017.pdf
    13 Jan 2017: 0 100 200 3001. 1.5. 2. 2.5. Time (s). YFP. /CFP. ... 2.5. YFP. /CFP. rat. io. 0 100 200 300 4001. 1.5.
  11. Bacterial actin MreB assembles in complex with cell shape protein RodZ

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embo2010RodZMreB.pdf
    17 Feb 2010: Before theexperiments, proteins were dialysed to 20 mM Tris–HCl, pH 7.5,1 mM EDTA, 1 mM sodium azide, 200 mM NaCl. ... Tris–HCl, pH 9.0, 1 mMEDTA, 1 mM NaN3, 200 mM NaCl, 2 mM TCEP and prespun at138 000 g for 10 min, 41C.

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