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8 Jun 2006: 150. 200. 247. Supplementary Figure 2. Sequence alignments and 3D models of C.

28 Sep 2022: Pi-PEG Screen 1.5x Precipitant Buffer Precipitant 2Condition Chemical Concentration Units Chemical Concentration Units pH Chemical Concentration Units 1-1 PEG 200 33.75 % Sodium acetate trihydrate 75 mM ... PEG 1500 22.5 % Sodium acetate trihydrate 75 mM

2 Feb 2024: LMB Rising Talent Fellowship Additional Information 1. Is the LMB Rising Talent Fellowship only for people from a Black background? Yes, this scheme is designed to attract Black researchers. This Fellowship is open to people who self-identify as

18 Jan 2021: 50 Group Leaders. 400 postdoctoral/support scientists. 100 PhD students. 200 support staff.

2 Oct 2014: w/v) PEG 3000 100 mM Imidazole/ Hydrochloric acid pH 8.0 200 mM Lithium sulfate. ... sodium tartrate 100 mM CHES/ Sodium hydroxide pH 9.5 200 mM Lithium sulfate.

11 Nov 2008: HeLa cells that had been serum-starved for 16 hours were stimulated with either EGF (20 ng/ml), TPA (200 ng/ml) or isoproterenol (10 µM) at 37ºC and the activation ... 60. 65. 70. 75. 200 300 400 500 600 700 800 900 1000 1100 12000.

2 Oct 2014: 200 mM Sodium chloride. F4 20% (v/v) PEG 300 100 mM Imidazole/ Hydrochloric acid pH 8.0 1000 mM Ammonium sulfate 10% (v/v) Glycerol. ... Tris base/ Hydrochloric acid pH 8.5 200 mM Magnesium chloride 10% (v/v) Glycerol.

26 Nov 2015: E12 Tetraethylene glycol, 80% v/v. F1 PEG 200, 20% v/v. F2 PEG 200, 40% v/v. ... F3 PEG 200, 80% v/v. F4 Pentaethylene glycol, 20% v/v. F5 Pentaethylene glycol, 40% v/v.

23 Sep 2016: Rhamnose monohydrate -E10 20 % v/v Tetraethylene glycol (TTEG) -E11 40 % v/v Tetraethylene glycol (TTEG) /-E12 80 % v/v Tetraethylene glycol (TTEG) F1 20 % v/v PEG 200 -F2 40 % ... v/v PEG 200 F3 80 % v/v PEG 200 F4 20 % v/v Pentaethylene glycol -F5 40 %

6 Apr 2007: on. Apr. il 6,. 200. 7 w. ww. sci. ence. mag. ... REPORTS. on. Apr. il 6,. 200. 7 w. ww. sci. ence.

26 Jun 2015: applicable). Organics (volatile)Hampton Research. Catalog #Supplied[ Stock ]. SuppliedVolume CAS #. 2-Propanol HR2-619 100 % 200 ml 67-63-0. ... Catalog #Supplied[ Stock ]. SuppliedVolume CAS #. (/-)-2-Methyl-2,4-pentanediol HR2-627 100 % 200 ml 107-41-5.

19 Apr 2006: Miroux personal communication), 200 AM dNTP’s (Roche), 1!reaction buffer,2.5 units PfuTurbok (Stratagene) in a total volume of 50 Al. ... TCF1 200 3–6 2.0 4.6 4/4 OK Yes nd. TCF2 200 3–6 2.0 4.6 4/4 OK Yes nd.

15 Jul 2016: The average weight of the crystallized proteins was 37kDa, including large complexes of 100- 200 kDa.

11 Nov 2011: Volume 22 May 15, 2011 1625. Cytosolic aggregates perturb the degradation of nontranslocated secretory and membrane proteinsOishee Chakrabarti, Neena S. Rane, and Ramanujan S. HegdeCell Biology and Metabolism Program, Eunice Kennedy Shriver National

19 Apr 2018: 200 200 0 5 µL 3.8 31 104 2min 10 sec. ... 2 96-well cellculture plate. 200 200 100 5 µL 3.8 31 200 3min 45 sec.

2 Oct 2014: A good starting point would be 50 µM (10 mg/ml for a 200 kDa protein).

21 Jun 2005: Buffer con-tained 100 mM PipesNaOH and 200 mM KCl (pH 6.8). ... 1B and 2. (Scale bar: 200 nm.). Schlieper et al. PNAS June 28, 2005 vol.

27 Sep 2022: Pi-PEG Screen 1.5x Precipitant Buffer Precipitant 2Condition Chemical Concentration Units Chemical Concentration Units pH Chemical Concentration Units 1-1 PEG 200 33.75 % Sodium acetate trihydrate 75 mM ... PEG 1500 22.5 % Sodium acetate trihydrate 75 mM

1 Jul 2011: Von großem Vorteil ist dabei diedynamische Instabilität der ParM-Filamente:Die sich schnell wiederholende Abfolge vonFilamentbildung und -zerfall (ungefähr 200-mal schneller als bei Aktin) ermöglicht esParM, nach den Plasmiden zu

13 Jun 2003: The protein was expressed in C41(DE3) cells and purified by using Ni2-NTA chromatography.After gel filtration (Sephacryl S-200 column in 200 mM NaCl20mM Tris5 mM DTT1 mM EDTA1 ... Themixture was gel filtrated (Sephacryl S-200 column in 20 mMTris5 mM DTT5

4 Jan 2006: fter. th. rom. bin cl. eava. ge o. f GST. ). 0.01. 0.1. 1. 10. 0 100 200 300 400. ln OD600. ... 1. 10. 0 100 200 300 400. ln OD600. pPLSC (E.coli LPAAT).

12 Oct 2006: c) Superdex 200 gel filtration of E. coli FtsKg in the presence of DNA. ... Storage buffer was. 20 mM Tris-HCl, 200 mM NaCl, 1 mM EDTA and 1 mM sodium azide.

9 Jan 2017: 200 nl protein 200 nl condition 100 nl additive). This late approach facilitates the screening at different concentrations of additives (by simply varying the volume of additive screen added) and implies

14 Nov 2021: min). Quantifoil Cu/Rh 200 mesh R2/2 grids. The same Lon. sample, diluted 10 times in buffer A (Lon concentration. ... Tris/HCl pH8.0, 200 mM NaCl, 10 mM MgCl2, 5 mM ATP.

2 Apr 2007: buffer at 200 V for 1.5 hours.

18 Dec 2008: on. Dec. embe. r 18. , 200. 8 w. ww. sci. ... embe. r 18. , 200. 8 w. ww. sci. ence. mag.

1 Mar 2024: CdpB2. SepF. SepFCdpB1. 200. Abso. rban. ce (a. u.). Abso. rban. ... For 1 transformation, 200 µl of the prepared cell suspension was used.

31 Jan 2020: Superose 6 Increase 3.2/300 column (GE Healthcare), equilibrated with buffer T200 (50 mM Tris-HCl, 200 mM NaCl, pH 8.0). ... equili-brated with 20 mM Hepes, 200 mM NaCl at pH 7.5.

13 Jan 2017: 0 100 200 3001. 1.5. 2. 2.5. Time (s). YFP. /CFP. ... 2.5. YFP. /CFP. rat. io. 0 100 200 300 4001. 1.5.

17 Feb 2010: Before theexperiments, proteins were dialysed to 20 mM Tris–HCl, pH 7.5,1 mM EDTA, 1 mM sodium azide, 200 mM NaCl. ... Tris–HCl, pH 9.0, 1 mMEDTA, 1 mM NaN3, 200 mM NaCl, 2 mM TCEP and prespun at138 000 g for 10 min, 41C.

20 Feb 2001: The protein is highlysoluble and stable in 200 mM sodium chloride-containing buffer, and runs as a monomer underthese conditions on size-exclusion columns (datanot shown). ... 20 % (w/v) glycerol,200 mM NaCl, and 0.004 % (v/v) DMSO.

28 Apr 2024: M446 (M443)F. G. 200 Å. Nanobody. Nanobody. Nanobody. Nanobody. Fig. 2. ... Scale bar, 100 nm.) The Insets (Scale bar, 200 nm.) in A and D show a close- up view of the area outlined by the dashed rectangle.

4 Mar 2019: 150 200 250 300 350 400 450 500. 0. 1. Residue number (S. ... 1. 900 1,000 1,100 1,200. 200 250 300 350 400 450.

3 May 2002: J. Cell. Biol.155, 193–200. f Takei, K. et al. (1999) Functional partnership betweenamphiphysin and dynamin in clathrin-mediated endocytosis. ... J. Cell. Biol. 155,193–200. 20 Takei, K. et al. (1999) Functional partnershipbetween amphiphysin and

13 Dec 2017: of tips Duration. 1 MRC plate 100 100 0 2 µL 2 16 104 2 min1 MRC plate 200 200 0 5 µL 3.8 31 104 2min 10 sec2 Costar ... plate 200 200 100 5 µL 3.8 31 200 3min 45 sec2 Costar plate 500 500 100 5 µL 7.4 60 200 4min 15 sec.

21 Mar 2003: 6. 0 F Glutamate release. 0.1 mM Ca2+. 200 400. Pro. ... Discussion). 0. 600. 800. 400. 200. 1000. Av AvPkCa2+(mM). Ionomycin (µM)30 mM KCl.

3 Sep 2014: 37uC. At mid-exponential growth phase, 200 ml of cell culturewere transferred to 1 ml of cold methanol and kept at 220uC forat least 60 min. ... Invitrogen) at a 1:200 dilution. Cells were observed using a Leica.

23 Dec 2013: Again BSA and 200 mMKCl were included throughout the gradient to increase speci-ficity. ... 7.5. 200. 2.5. 225 250(nm)λ. CD. [mde. g]. - liposomes+ liposomes.

26 Jan 2006: A lossof 100–200 l is usually observed. Purification of Dynamin and Its Mutants from E. ... Scale bars are 200 nm in. length. [52] IN VITRO RECONSTITUTION OF ENDOCYTIC STAGES 607.

3 May 2002: J. Cell. Biol.155, 193–200. f Takei, K. et al. (1999) Functional partnership betweenamphiphysin and dynamin in clathrin-mediated endocytosis. ... J. Cell. Biol. 155,193–200. 20 Takei, K. et al. (1999) Functional partnershipbetween amphiphysin and

12 Nov 2009: TEN 200 is a buffer containing 20 mM Tris, 1 mM ethylenediaminetetraacetic acid (EDTA), 1 mM sodium azide and 200 mM sodium chloride. ... 1998) G04 oCNVA Concanavalin A 7.0 26.5 Sigma, L7647 Dissolved in TEN 200.

19 Nov 2004: sZ-N. TmFt. sZ-C. TmFt. sZ-N. C. TmFtsZ TmFtsZ-N. TmFtsZ-C TmFtsZ-NC. Wavelength (nm)200 210 220 230 240 250 260. ... 081% (w/v) malachite green and 2 ml of water were mixed and 200 µl was added to 50-µl reactions contain-ing proteins or inorganic

3 Dec 2005: stops when more than 200 pixels are inside the region. The directional dilation forces. ... 200 eggs from a variety of mutant types and recording conditions illustrate the.

26 Nov 2012: In some experiments of this sort, using a range 0 to 200 V, the potential was found to vary along the thread proportionately to the electron flux. ... manner. The corresponding interference patterns indicate a n ear-zero potential, a high positive

6 Dec 2014: 200. 250. MinDATP. ATP/ATPγS. EcMinD (D154A): non-polymerising. D155A. minB – minB – slmA–. ... 200. 250. 300. Inte. nsity M. Figure 3 | MinCD filaments bind to membrane and are disassembled by MinE.

10 Sep 1999: mukB V.cholmukB H.inflmukB Y.pestmukB E.coli. S6 H4 H5 H6. ... Protein was eluted with the same buffercontaining 400 mM imidazole. The protein was applied to a sephacrylS-200 column (Amersham-Pharmacia) equilibrated in 20 mM Tris,200 mM NaCl, 1 mM

5 Mar 2001: The protein was loaded directly onto a Sephacryl S200column (Amersham-Pharmacia) equilibrated in 200 mM NaCl, 20mM Tris, 1 mM EDTA and 1 mM NaN3 pH 7.0.

30 Jun 2004: Spo0J] (mM)0 25 50 100 200 300 25 50 100 200 300. ... The protein was buffer exchanged into20 mM Tris, pH 8.5, 200 mM NaCl using a Sephadex G25 gelfiltration column (Amersham).

29 Dec 2017: anti-biotin antibody. F 127. catastrophe time. 0. 0.5. 1.0. time (seconds) 200 800. ... of the reaction was pipetted onto freshly glow-dischargedCu/Rh Quantifoil R2/2 200 mesh holey carbon grids (Quantifoil).

28 Nov 2006: Acetyl Transferase Assays. GST-MEK2 (0.5 g) (purified by usingstandard protocols for GST-tagged protein purification) wasincubated with varying amounts (0 –200 ng) of (His)6-YopJ-wt ... Stimulation of MEK and Erk phosphorylation by various