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2 Oct 2014: w/v) PEG 3000 100 mM Imidazole/ Hydrochloric acid pH 8.0 200 mM Lithium sulfate. ... sodium tartrate 100 mM CHES/ Sodium hydroxide pH 9.5 200 mM Lithium sulfate.

3 Sep 2014: 37uC. At mid-exponential growth phase, 200 ml of cell culturewere transferred to 1 ml of cold methanol and kept at 220uC forat least 60 min. ... Invitrogen) at a 1:200 dilution. Cells were observed using a Leica.

2 Oct 2014: 200 mM Sodium chloride. F4 20% (v/v) PEG 300 100 mM Imidazole/ Hydrochloric acid pH 8.0 1000 mM Ammonium sulfate 10% (v/v) Glycerol. ... Tris base/ Hydrochloric acid pH 8.5 200 mM Magnesium chloride 10% (v/v) Glycerol.

6 Dec 2014: 200. 250. MinDATP. ATP/ATPγS. EcMinD (D154A): non-polymerising. D155A. minB – minB – slmA–. ... 200. 250. 300. Inte. nsity M. Figure 3 | MinCD filaments bind to membrane and are disassembled by MinE.

2 Oct 2014: A good starting point would be 50 µM (10 mg/ml for a 200 kDa protein).

14 Nov 2014: loaded onto a 16/600 Superdex 200 gel filtration column (GE Healthcare) in 50 mM Tris pH 7.5, 150 mM NaCl, 1 mM βME. ... Crystals were cryo-protected by adding reservoir solution supplemented with 50-60 % (w/v) PEG 200, before flash freezing in liquid

6 Jun 2014: Bar: 200 nm. (B) Cryo-EM image of a lipid tube. Bar: 20 nm.

31 Dec 2014: elifesciences.org. Szwedziak et al. eLife 2014;3:e04601. DOI: 10.7554/eLife.04601 1 of 22. Architecture of the ring formed by the tubulin homologue FtsZ in bacterial cell divisionPiotr Szwedziak†, Qing Wang†, Tanmay A M Bharat, Matthew Tsim, Jan