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6 Jan 2005: between Km and Kd, which wefound to be equal within error (200 mM). ... with 5 mM MDCC–PBP in 200 ml 50 mM Tris–HCl, pH 7.4.Data were fitted to:.

8 Feb 2008: 225 nm. Conditions: 25C in 50 mM Tris-HCl. (pH 7.5), 200 mM KCl, 10% (v/v) glycerol,. ... 200 mM KCl, 10 % glycerol (v/v), 1 mM DTT, except for the ITC exper-.

23 Aug 2004: 2: A280 0.3 at (X) 13,200 rpm, (B) 14,500 rpm, (V)16,000 rpm, and (O) 18,700 rpm. ... the Hsp90–Cdc37 complex on an analytical Superdex 200 PC 3.2/30 gel filtration column at 4 8C.

26 Jul 2010: 200 lm laser spot and 1 kHz laser pulsing. Photo-bleaching was only observed at flowrates up to 1 lLmin1 (see Supporting Information Fig. ... Crystalaser, Reno, NV) emitted a 200 lm diameterband-pass filtered (BG3, Newport) ray perpendicular.

8 Feb 2008: at pH 7.5 in a buffer containing 200 mM KCl and10% (v/v) glycerol. ... Conditions: 25 C, 50 mM Tris–HCl(pH 7.5), 200 mM KCl, 10% (v/v) glycerol, 1 mM DTT.

16 Nov 2006: 0.0. -0.5. 1.0. -1.5. -2.0. 0 100 200 300 400 500 600. ... 145151. 39. 200. 182. 143. 143. Folding of green fluorescent protein.

4 Jun 2008: Bound protein was eluted witha linear salt gradient over 200 mL from 0 to 2 M NaCl.Protein was further purified by gel-filtration chromato-graphy on either a G75 Sepharose ... Protein interaction assays. Protein interaction was assayed according to

17 Jul 2005: No slow, Proisomerization phases were observed in the refolding experimentsover a 200-s time scale.

10 May 2010: YibKSHSH and YbeASHSH were fully reduced [5 mM. tris(2-carboxyethyl) phosphine] in either native [50 mM Tris-HCl (pH 7.5),200 mM KCl, 10% (volvol) glycerol] or denaturing

8 Dec 2008: All experiments were performed in abuffer of 50 mM TrisHCl (pH 7.5), 200 mM KCl, 10% glycerol (vol/vol), 1 mMDTT, except for ITC experiments where -mercaptoethanol replaced DTT asthe