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7 Dec 2006: A pre-culture, grown in 2¥ TY, was used in a1:1000 dilution to inoculate 200 ml of minimal medium con-taining 1¥ M9 supplemented with 0.4% glucose, 2 mMMgSO4,

14 Sep 2010: Many bacterial plasmids are maintained at low copy number (1–5 copies per chromosome equivalent). To prevent plasmid loss at cell division, plasmids have evolved active segregation mechanisms that are loosely analogous to the spindle apparatus in

12 Oct 2012: Superstructure of the centromeric complex ofTubZRC plasmid partitioning systemsChristopher H. S. Aylett and Jan Löwe1. Medical Research Council Laboratory of Molecular Biology, Division of Structural Studies, Cambridge CB2 0QH, United Kingdom.

4 Oct 2010: Bound material was elutedfrom the resin using 10 ml of 2 N hydrochloric acid, lyophilizedto remove the acid, and taken up in 200 l water. ... time in a 200-l volume using a FluoroMax-2spectrofluorometer (Horiba Yvon Spex).

18 Jan 2019: RESEARCH ARTICLE. The ER membrane protein complex promotes biogenesis ofsterol-related enzymes maintaining cholesterol homeostasisNorbert Volkmar1,, Maria-Laetitia Thezenas2, Sharon M. Louie3, Szymon Juszkiewicz4, Daniel K. Nomura3,Ramanujan S.

8 Jun 2020: ATPɣS (translocating state). A. 0 100 200 300 400 500. 0.

7 Mar 2019: P1P2. GCN2. uL10. A. 200. kDa. 15012010085. 7060. 50. 4030. 25201510.

23 Jan 2012: raster, different portions o f the image that were between 1,200 and 6,000 A from a reference area were searched for maximum correlation.

12 Nov 2006: Briefly, a 1 ml sample of each PX domain was loaded onto a Superdex 200 column (Amersham-Pharmacia) equilibrated and eluted with 20 mM Tris-HCl buffer, pH 7.4, containing

4 Mar 2008: coli FtsQ and L. monocytogenes FtsQ, in20 mM Tris, 1 mM EDTA, 1 mM NaAzide, 200 mM NaCl,pH 7.0 (TEN200) for Y.

4 Feb 2015: Finally, we also con-sidered that force generation may be delayed from muscleactivation; we performed parameter sweeps from 0 to 200 ms delay.

18 Jan 2021: 50 Group Leaders. 400 postdoctoral/support scientists. 100 PhD students. 200 support staff.

19 Aug 2023: 4c). ΔGapp9.8–0.4–3.7. ΔGapp1. –0.32. –0.33. –3.7. YIPF1(1–200) Sec61β(201–221)N. Flag1 2. YIPF1. ... The 221-residue Flag–YIPF1(1–200)–Sec61β series was generated by Gibson cloning DNA fragments (Twist Biosciences) into a

16 May 2013: 200 nucleotide poly(A) tail.This suggested stalling of ribosomes after partial translation. ... 200 nt poly(A) tail. 33P-labeled transcripts weregenerated with 33P-UTP (Perkin-Elmer) in transcription reactions.

23 Sep 2011: 08907 L’Hospitalet de Llobregat, Spain.J. L. Gallop’s present address: Harvard Medical School, Department of Systems Biology, 200 Longwood Avenue,. ... For tubulationassays, typically 1 mg/ml liposomes (200 nm) were incubated for 10 minwith 10, 20,

16 Oct 2008: More than 200 analogs of these hits wereiteratively synthesized through diversification of the R1 and R2substituents (Supplementary Table 1). ... We profiled PP121 and PP487against over 200 protein kinases and found that they inhibit a patternof tyrosine

5 Dec 2012: Subsequently, Gb1His-g2 dimers were eluted with abuffer containing 20 mM tris-HCl (pH 8.0), 25 mM NaCl, 0.1% C12E10,200 mM imidazole, and 10 mM 2-ME. ... ME. Gb1His-g2(C68S) mutants were eluted with abuffer containing 20 mM tris-HCl (pH 8.0), 25 mM NaCl,

12 Jan 2012: Example:occupancy group id 1 chain A # chain A belongs to occupancygroup 1occupancy group id 1 chain B residues from 200 to 500 # all residues between residues200 and 500 of chain ... 150 A atoms C # all atoms with atom namesstarting with C for all

3 Aug 2009: α5.α9 (130.219). α8.α8 (200.205). D57N. C285S/C288S. WTL9. 4DM. 97D. M10. 0D. ... NADH, and 2 mM Get3 (wild-type or mutant), and reactions were carried out ina final reaction volume of 200 ml.

5 May 2019: ility FtsL. 50 100 150 200 2500.0. 0.2. 0.4. 0.6. 0.8. ... 100. 200. 300. 400FtsB (22-103) FtsN (57-319) vs FtsQ. FtsN (57-319) vs FtsQ.