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1 Apr 2016: Each image of a tube consisted typically of 1200 X 200 pixels ( 5 000 X 1000 A), and was padded to a 1536 X 512 array size, from which Fourier ... Second-order effects, e.g. mul-tiple scattering, were barely detectable in the nega-tive stain study, where

4 Jan 2006: fatty acid-free BSA and 0.01% Triton X-100 with substrate concentrations (20–200 mM LPA) at 25 8C and 37 8C with 14C-labelled oleoyl-CoA (Amersham) at aspecific ... J. Cell Biol. 155, 193–-200 (2001). 11. Huttner, W. B. & Schmidt, A.

3 Dec 2004: interactions. J. Cell Biol. 155, 193–200 (2001). 20. Zhang, B. et al.

16 Oct 2009: REPORTS. on. Oct. ober. 18,. 200. 8 w. ww. sci. ence. ... Pdl indicates the polydispersity index. The actual sizeof the liposomes is significantly smaller for the 800 and 200 liposomes suggestingthat the actual curvature preference of the synaptotagmin

13 Jun 2022: 150. 50. 250. mAU. 100. 200. 150. 50. 250. mAU. elution volume (ml)5 1510 20. ... 12.4 mlβ-amylase(200 kDa). 18.9 mlCytochrome c. (12.4 kDa). 17.4 mlCarbonic anhydrase.

13 Sep 2000: Figure 2D) as published earlier (Löwe and Amos, 1999).Tubes assembled in the presence of GMPCPP show re-peats of 200 Å, 100 Å, 67 Å, and 50 Å (Figure 2E, ... In the case of the GMPCPP-inducedtubes, groups producing the 200 Å longitudinal

19 Dec 2016: 200 mM NaCl, 1 mM DTT, 10% glycerol (v/v), 10 mM imidazole, pH 8.0) supplemented with DNase. ... Stock et al., 2005). After optimisation, the best crystals were obtained in 200 nl drops composed.

18 Dec 2022: The cell pellet was resuspended in 1 pellet volume of Buffer A (50 mMTrisHCl, pH 8.0, 200 mM NaCl, 5% glycerol, and 5 mM 2-mercaptoethanol)with protease inhibitors (Roche) ... The incubated samples were supplemented with 0.1% (wt/vol)β-octyl glucoside

9 Dec 2016: Why? Hint, hint! Increased to 200 Conformers. Problem 1: (Back to Acedrg dictionary):Use Fixed Atoms.

28 Sep 2006: 50 nm. 100 nm. 200 nm. I. Supplementary Figure 2. The crystal structure of BDLP in GDP-associated and nucleotide-free conformation.

30 Jan 2005: mol. Pi p. rodu. ced/. mol. Soj. 0 50 100 150 200.

3 Dec 2004: AP2was dialyzed against 50 mM triethanolamine-KCl,pH 8.0, 200 mM NaCl, 0.2 mM EDTA, 0.1% b-mer-captoethanol, and 0.02% NaN3.

4 May 2001: 200 column buffer. ... solution, andadditionally 200 mM NaI in the drop.

6 Apr 2017: Crystals wereflash-frozen in liquid nitrogen for data collection using an additional 25% (vol/vol)PEG 200 as cryo-protectant. ... 200 mesh grids in a Vitrobot Mark IV (FEICompany) maintained at 10 C at 100% relative humidity.

18 Feb 1999: 110 120 130 140 150 160 170 180 190 200. 210 220 230 240 250 260 270 280 290 300.

6 Sep 2010:  reservoir holds 200 µl.  use robot or manually dispense 0.1 - 10 µl of reservoir and protein.

21 Nov 2012: ni. WT/. icW. T/i. 0. 100. 200. 300. 400. CD/n. ic. ... 100. 200. 300. ΔReq. (R. U). 0100200300400500600700800. SH2 deletion lipid kinase dead cancer-linkedmutations:.

17 Mar 2004: J. Cell Biol. 155,193-200.

23 Dec 2013: 1). An 200-amino-acid N-terminal do-main (FtsKN), which has four or five membrane-spanning seg-. ... C) Sizeexclusion chromatography-multiangle light scattering (SEC-MALS) analysis of TtFtsKN. Using a Superdex S-200 size exclusion column, samples were

15 Mar 2012: ParB (200 RU) was immobilized on an SPR sensor chip and allowed to react with wild-type MipZ protein (0.5 µM) that had been preincubated with 2 mM ATP and ... Subsequently, the protein was applied to a Sephacryl S-200 gel filtration column (GE Healthcare

20 May 2010: The EMBO Journal, (2010). doi:10.1038/emboj.2010.99

14 Nov 2014: loaded onto a 16/600 Superdex 200 gel filtration column (GE Healthcare) in 50 mM Tris pH 7.5, 150 mM NaCl, 1 mM βME. ... Crystals were cryo-protected by adding reservoir solution supplemented with 50-60 % (w/v) PEG 200, before flash freezing in liquid

29 May 2019: A) Cells expressing GFP-PrP and GFP-PrP were incubated on. ice with 200 nM extracellular Alexa647-conjugated Nb and Alexa647 fluorescence was measured by flow cytometry. ... pro. port. ion o. f cells. time (min). 0 50 100 150 200.

11 Aug 2020: itotic. chro. mos. omes. Centrifugation 200 g. Supernatant containingchromosomes. Centrifugation 18,000 g(to pellet chromatin).

15 Apr 2004: Calcu-lations employed torsional dynamics at 50 000 K for 1000steps of 15 ps, then a first slow cooling stage of torsionaldynamics (200 cycles each of 1000 steps of 15

23 Oct 2012: The image pixel size at this. magnification was 1.64 Å/pixel. In total of 200 CCD images were collected. ... µl were added to the 200 µl reaction mixture before initiating polymerization with 2 mM (final concentration).

6 Mar 2001: For the continuousenzyme-coupled assay, purine nucleotide phosphorylase (20 U ml-1) and 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG) (200 mM) were also present in thereaction mix from the

5 Jul 2010: 3). Clearly visible are : (a) "rings" which have an insidediameter close to 800 A and an outer diameter the same as thatofthe pore complex itself(- 1,200 A) ; (b) large particles

16 Aug 2004: Tris (pH 7.5),200 mM NaCl and 5 mM MgCl2 and eluted separately.Following calibration, 10 ml of ZapA protein at 10 mg/ml was injected and eluted as a ... 1 mM EDTA,1 mM NaN3 and 200 mM NaCl was placed in thesample compartment of an Epon double-sector

27 Jan 2018: Briefly, cells were washed three times in PBS and collected by spinning at 200 x g for 5 min. ... The resin was washed five times with 0.1% digitonin, 200 mM NaCl, 50 mM HEPES.

3 Dec 2004: AP2was dialyzed against 50 mM triethanolamine-KCl,pH 8.0, 200 mM NaCl, 0.2 mM EDTA, 0.1% b-mer-captoethanol, and 0.02% NaN3.

17 Jul 2009: 3. Farsad K. et al. J. Cell Biol. 155, 193–200 (2001).4.

4 Aug 2017: 1, A and B). Cross-linking reactionsof the active fractions revealed interacting part-ners of 200, 120, and 100 kD (Fig. ... The gradients were either 2 ml or 0.2 ml, on which either 200 µl or 20 µl of sample were layered.

16 Jul 2023: STK strains were supplemented with 200 μM EdU(Thermo Fisher Scientific, C10639) for 10 min. ... Three microliters of resus-pended cells were applied to a freshly glow-discharged QuantifoilAu 200 mesh R2/2 or R2/1 grid.

1 Dec 2010: Smc1DDSmc3AAA. ARS207 ARS231. 150 160 170 180 190 200. 10.0. 5.01.00.0. ... 5.0. 1.00.0. Smc1DD E1158QSmc3AAA. ARS207 ARS231. 150 160 170 180 190 200.

3 Dec 2004: interactions. J. Cell Biol. 155, 193–200 (2001). 20. Zhang, B. et al.

20 Apr 2002: 21 ions. Enzyme(200 ng) was added to the subphase, via an injection port in the Teflontrough, 5 min after the monolayer was spread to allow the surfacepressure to stabilize. ... Replacement of these residues byalanine resulted in a great reduction of the

11 Oct 2007: After precipitation in 30% ammonium sulfate saturationand anion-exchange chromatography, the protein wasdialyzed against 50 mM Tris–HCl (pH 8.0), 200 mM KCl,1 mM EDTA, 10% (v/v) glycerol ... Drops were composed of100 nl of protein at 10.0 g/l (with 8

27 Apr 2015: Protein aggregateswere removed by a second spin at 200,000 g for 10 min. ... 200 pM) was allowed to bind for 10–15 min; this was followed by.

20 Jul 2011: mM Tris pH 7.5, 1 mM EDTA, 1 mM sodium azide and 200 mM NaCl). ... with buffer A being (20 mM Tris pH 8.5, 500 mM NaCl, 1 mM EDTA, 1 mM TCEP) and buffer B (20 mM Tris pH 8.0, 200 mM NaCl,

17 Jan 2006: Bars, 200 nm. 4535Sorting Nexin-2 and CI-MPR retrieval. mammalian orthologue of the yeast retromer componentVps17p (Haft et al., 2000). ... J. Cell Biol. 155, 193-200. Gagescu, R., Gruenberg, J. and Smythe, E.

17 Mar 2003: O.B., Jr., Krupnick, J.G., Vsevolod, V., Gurevich, V.V.,solution (30% PEG MME 5000, 200 mM (NH4)2SO4, 100 mM MESBenovic, J.L., and Keen, J.H. ... Hao, W., Tan, Z., Prasad, K., Reddy, K.K., Chen, J., Prestwich, G.D.,vent consisting of 40% PEG 400, 200 mM

20 Apr 1999: Twenty four micrographs out of 200 in the tilt range of 0–60 wereselected and scanned on a Zeiss SCAI scanner with a 28µm step size.Images were processed with the

11 Apr 2024: In "Development and Neurobiology of Drosophila", eds Siddiqui O, Babu P, Hall LM & Hall JC, Plenum Press, New York and London, pp 183-200.

16 Aug 2008: tion plates mixing 200 200 nl of protein and crystallization solution. ... KOPS1 DNA. Crystallization solution was 200 mM citric acid/NaOH pH 3.5.

30 Apr 1999: 2,000. 500. a. Time (min). Time (min). 200. µM GTP. GT. ... ferase-tagged amphiphysin-2 SH3 domain on glutathione–agarose beads at 4 C. After extensive washing of the matrix in buffer A (200 mM NaCl, 20 mM HEPES, pH 7.3,

6 Oct 2004: indicated dishes were treated with 200 nM wortmannin 40 minRap on ARAP3 Rho GAP activity (Figure 3C).

30 Apr 2010: 4. 100 mM CaCl2: Dissolve in water. Keep at 4C.5. 200 mM EGTA. ... A good range to start is between 30and 200 µL of packed bead volume.

25 Nov 2019: Nature Structural & Molecular Biology, doi:10.1038/s41594-019-0331-x

9 May 2003: concentration of approximately 200 nM. Combined with the lack of putative voltage gated Na+ channels in the C.elegans. ... change, corresponding to an apparent free calcium concentration of approx. 200 nM.