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15 Sep 2020: For correspondence:. jyl@mrc-lmb.cam.ac.uk (JL);. ashley.nasmyth@bioch.ox.ac.uk. (KAN). †These authors contributed. equally to this work. Competing interests: The. authors declare that no. competing interests exist. Funding: See page 33. Received:

7 Apr 2010: FEBS Letters 584 (2010) 1830–1839. j o u r n a l h o m e p a g e : w w w. F E B S L e t t e r s. o r g. Review. Modeling membrane shaping by proteins: Focus on EHD2 and N-BAR domains. Felix Campelo a,b, Gur Fabrikant a, Harvey T. McMahon c,

15 Feb 2007: Lysatesfrom HSG cells were then prepared as described previously[30]. Briefly, whole cell lysates were centrifuged through a10–50% sucrose gradient and 200 μl fractions weresequentially removed from the centrifuged lysate. ... The indicated protein

2 Aug 2010: After a wash step with buffer A, the column was eluted using a gradient from 0–100% buffer B (buffer A 200 mM imidazole).

24 Jan 2009: Molecular Biology of the CellVol. 19, 5093–5103, December 2008. Synaptotagmin-1 Utilizes Membrane Bending and SNAREBinding to Drive Fusion Pore ExpansionKara L. Lynch, Roy R.L. Gerona, Dana M. Kielar, Sascha Martens,†Harvey T. McMahon,† and

5 Sep 2003: The resin was washed with buffer Acontaining “front” face. These results suggest that vari-and then buffer B (50 mM Tris, pH 7.5, 200 mM NaCl, and 5 mMous

12 Jun 2020: 6Y4L). The heterodimer consists of EMC2 (residues 11–274), depicted in blue, and EMC9 (residues 1–200),. ... of atoms 9412. Protein residues 1906. Ramachandran Outliers (%) 0.49. Mean B-factors (2) 200.0.

23 Nov 2004: N-BAR SH3. GTPase PH GED. PTB. ATPase. 200 amino acids. EH EH. ... 1.9(1.1) No bindingEpsin1-P1 APAFSDPWGGSP 200(1.2).

14 Jul 2021: For correspondence:. jyl@mrc-lmb.cam.ac.uk (JL);. ashley.nasmyth@bioch.ox.ac.uk. (KAN). †These authors contributed. equally to this work. Competing interests: The. authors declare that no. competing interests exist. Funding: See page 28. Received:

16 May 2008: 12, Vac1 and EEA1) and that binds to the membrane lipid phosphati-dylinositol-3-phosphate. Dense-core ... Double C2-like-domain-containing proteins. Synaptotagmin-like proteins. Multiple-C2-domain and transmembrane-domain proteins. 200 amino

12 Nov 2006: Further purifi cation was by gel fi ltration on Superdex 200 in 10 mM Tris-Cl, pH 8.0, 0.15 M NaCl, 1 mM DTT, and 1 mM EGTA and

15 Jan 1998: 433 440 (Tb4) 1.15 (8)V 173 178 498 503 (Tb5) 3.36 (6)Vb 182 188 VI 193 200 518 524 (Tb6) 1.10 (5)VII 226 235 546

25 Sep 2013: input IP. GFPAni1-409GFP. SyndFLAG. Ani1-409GFP. GFP. 200. valu. e. 100. 200. ... Scale bars represent 1 mm and 200 nm for low magnification and high magnificationimages, respectively.

6 Mar 2008: UNIVERSITY OF CALIFORNIA, SAN DIEGO. Tracking and Analysis of C. elegans Behavior Using Machine Vision. A dissertation submitted in partial satisfaction of the requirements for the degree Doctor of Philosophy. in. Electrical and Computer Engineering

5 Jan 2016: 5, 200 mM KOAc, 15 mM MgOAc2, 1 mM DTT). ... The samples were then transferred to a micro-spin column and washed with 100 volumes (relative to the resin volume) of wash buffer (50 mM HEPES pH 7.5, 200 mM

24 Sep 2004: The high-spin. Acknowledgmentssupernatant was loaded in batch onto Glutathione Sepharose4B.The resin was washed with buffer B (50 mM Tris [pH 7.5] and 200.

13 Jun 2009: RING domain (termed MgrnDR) still interacts with PrP. Append-. ing only residues 200–250 of Mgrn to an FP was sufficient to. ... brain to be at least 2 nmol (Pan et al., 1992) and up to 6 nmol(Bendheim et al., 1988) per gram (i.e., 70–200 mg PrP

14 Jun 2006: 200-fold more potently than p110b. p110a inhibitors. also blocked activation of the mTORC1 pathway as mea-.

13 Dec 2008: The Hydrophobic Insertion Mechanism of Membrane CurvatureGeneration by Proteins. Felix Campelo,y Harvey T. McMahon,z and Michael M. KozlovDepartment of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv,

24 Sep 2015: S. (2004) J. Membr. Biol. 200,. Regulation of SOCE. 12184 JOURNAL OF BIOLOGICAL CHEMISTRY VOLUME 282 • NUMBER 16 • APRIL 20, 2007.

17 Aug 2020: d. e. 1 200. – UV:. f. β1ARTMD1. Cytosol. Asterixcrosslink. –. g. ER lumen. IP: UBQL. N2. SRP5. 4. Sec6. 1. Aste. ... The elution samples are derived from approximately 200-fold more rough microsomes than the input and f low-through (FT) samples.

15 Jan 2004: Samples were treatedwith PI (200 g of aprotinin and leupeptin/ml and 5 mM phenylmethylsulfonylfluoride), transferred to five volumes of preheated PK inactivation buffer (1%sodium dodecyl sulfate [SDS], 0.1 ... E to H) Brain homogenates from

30 May 2008: 0.5 M sucrose in PSB, 200 µL) by centrifugation at 100,000 rpm for 45 min at 4 C in a.

11 Nov 2019: e.g. 200 A2) before the fitting steps, as blurring allows a larger radius of.

5 Aug 2013: Deubiquitinases Sharpen SubstrateDiscrimination during MembraneProtein Degradation from the ERZai-Rong Zhang,1,3, Juan S. Bonifacino,1 and Ramanujan S. Hegde2,1Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child

11 Aug 2015: The proportion of each 200 ms translationcycle during which RNCs are compatible for Alu domain binding remains to be determined.Regardless, a twenty residue TMD would have approximately 21 translation cycles

30 Nov 2006: Substrate-Specific TranslocationalAttenuation during ER Stress Definesa Pre-Emptive Quality Control PathwaySang-Wook Kang,1,4 Neena S. Rane,1,4 Soo Jung Kim,2 Jennifer L. Garrison,3 Jack Taunton,3. and Ramanujan S. Hegde1,1 Cell Biology and

15 Jul 2005: domain. 11697. 66. 200. 45. tota. l RM. RM. D. BCsu. pern.

11 Jun 2013: On screen: 200 dpi On paper: 300 dpi But for line figures: 1000 dpi (Guide to authors: “Non-‐vector graphics should be preserved at high resolu-on: 300 dpi minimum at

24 Jul 2020: For correspondence:. rhegde@mrc-lmb.cam.ac.uk. †These authors contributed. equally to this work. Competing interest: See. page 25. Funding: See page 25. Received: 15 June 2020. Accepted: 10 July 2020. Published: 13 July 2020. Reviewing editor:

23 May 2012: 160 nM), or a Hsp70 chaperone mixture contain-. ing Hsp70 (150 nM), HOP (200 nM), Hdj1 (300 nM),.

18 Sep 2008: Note that the death of some animals (in both trans-. genic lines) by 200 days is unrelated to the PrP transgenes orto neurodegenerative illnesses.

1 Aug 2010: The translation timefor RNC preparation was 15 min at 32 uC, after which the samples (volumetypically 200 ml) were chilled on ice and immediately layered on 2-ml 10–50%. ... Centrifugation was for 1 h at55,000 r.p.m. at 4 uC in a TLS-55 rotor (Beckman),

23 Nov 2004: 11.7 0.3. -6.9 0.5. Epsin1-P APAFSDPWGGSP 200.0 1.2 5.0e3 -10.0 -5.2.

16 Oct 2003: Over 200 COS-7 cells expressing dimerizing GFP-Sec61 were imaged using the same settings for all cells.

16 Jul 2023: The exchanged tubulin wasthen injected onto a Superdex 200 3.2/300 column (GE Health-Care), equilibrated, and eluted in 80 mM K-Pipes, 100 μM Na-GDP, 100 μM MgCl2, ... A total of 2.5 μl was appliedto a gold-on-gold grid (UltrAuFoil R2/2 200 mesh),

13 Apr 2015: The dilution. buffer contained 250 mM sucrose, 50 mM. HEPES (pH 7.4), and 0, 200, 400, or 600 mM.

15 May 2007: # 2007 The Authors. doi: 10.1111/j.1600-0854.2007.00578.xTraffic 2007Blackwell Munksgaard. The Conserved Isoleucine–Valine–PhenylalanineMotif Couples Activation State and EndocyticFunctions of b-Arrestins. Anne Burtey1,2, Eva M.

28 Sep 2007: 40 80 120 160 200. 20. 40. 60. 80. 100. Time (min).

15 Apr 2004: JCB. Article. The Journal of Cell Biology, Volume 164, Number 7, March 29, 2004 997–1007http://www.jcb.org/cgi/doi/10.1083/jcb.200312079 997. The organization of engaged and quiescent translocons in the endoplasmic reticulum of mammalian cells.

19 Jul 2011: 3a). The size of clathri n-coated vesicles depends on the size of its cargo76, with an observed upper limit of about 200 nm external diameter, as in the case of

9 Sep 2015: elife.elifesciences.org. MacKinnon et al. eLife 2014;3:e01483. DOI: 10.7554/eLife.01483 1 of 23. An allosteric Sec61 inhibitor traps nascent transmembrane helices at the lateral gateAndrew L MacKinnon1†, Ville O Paavilainen1†, Ajay Sharma2,

16 Feb 2010: JCB 6 of 12. Because Prl-PrP(AV3)11 develops disease at earlier ages than PrP(A117V)H mice ( 200 d versus 570 d), we compared their respective CtmPrP levels more

16 Jul 2008: Stable celllines were generated by selection in 200 g/ml Zeocin (Invitrogen, Carlsbad,CA) for 4 wk, followed by subcloning of individual colonies.

6 Dec 2004: Indeed, release ofinsulin has been proposed to involve the release of the crystallinedense core (100 –200-nm diameter) essentially as an intact struc-. ... that the crystalline core of insulin secretory vesicles (100 –200-nm diameter) is ever

2 May 2013: mAU. 10 μM 5 μM. 1.5 μM. 5 10 15 200.

26 Aug 2021: 1:200. Ms anti-TUBB Sigma-Aldrich. T7816 AB_261770. - 1:300. Recombinant proteins Full length HERC1, the various HERC1 mutants, PAAF1, and PSMD10 were purified from Expi-293 cells via

27 Apr 2009: Caveolin1 is enrichedin caveolae (11), with 100–200 moleculesper caveola (37), and cells that do not ex-press this protein (and muscle cells not express-ing caveolin3) are devoid of morphologicallyevident

12 Oct 2012: Scale bars,200 nm in panel A and 20 m in panels B through D.

11 May 2007: Consider that a homogeneouslydistributed fluorescent protein must be presentat 200 nM to be visualized over backgroundcellular fluorescence (Niswender et al., 1995).Thus, experimental questions involving cellu-lar expression of a fluorescently