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Science Journals — AAAS
https://haseloff.plantsci.cam.ac.uk/resources/Part2SynBio_refs/Synthetic-yeast-genome/Zhang2017_rDNA.pdf14 Aug 2023: 1 mM EDTA,pH 8.0, 2% (v/v) Triton X-100, 1% (w/v) SDS].100 mL of 0.5 mm Glass Beads (Biospec, 11079105)and 200 mL of ... The pellets weredissolved in 200 mL TE buffer, phenol extracted,precipitated in 500 mL 100% ethanol with 70 mL3 M sodium acetate. -
pnas201311166 15985..15990
https://haseloff.plantsci.cam.ac.uk/resources/Part2SynBio_refs/Lecture-3/Niederholtmeyer2013.pdf14 Aug 2023: 1200. 800. 400. 02520151050. 2. 1. 02520151050. 800600400200. 02520151050. 200. 100. ... 200. 100. 0. 200. 100. 06050403020100 6050403020100 6050403020100 6050403020100. Prot. ein. -
Beyond editing: repurposing CRISPR–Cas9 for precision genome…
https://haseloff.plantsci.cam.ac.uk/resources/Part2SynBio_refs/Lecture-1/Dominguez2016.pdf14 Aug 2023: system, the optimal window was determined to be between -200 and 1 bp relative to the TSS49. -
REV_ISS_WEB_PBI_12016_11-3 325..335
https://haseloff.plantsci.cam.ac.uk/resources/Part2SynBio_refs/Lecture-4/Yang2013.pdf14 Aug 2023: mL screw-cap tubes andmixed to 200 lL of water or 175 lL of NaOH (1%, w/v) for hotwater or dilute alkaline pretreatments, respectively, incubated at30 C for 30 min -
OP-ANNB150080 1..16
https://haseloff.plantsci.cam.ac.uk/resources/Part2SynBio_refs/Lecture-1/Paris2015.pdf14 Aug 2023: Hebrew literature (200–400 CE). Three large codices of JewishLaw were compiled in Israel during the first centuries CE. ... Theyare easily searched on-line (Mekhon Mamre, 2015). TheMishna, compiled by Rabbi Yehuda the President in northernIsrael around -
Science Journals — AAAS
https://haseloff.plantsci.cam.ac.uk/resources/Part2SynBio_refs/Synthetic-yeast-genome/Xie2017_Perfect_SynV.pdf14 Aug 2023: An equal volume ofglass breads and 200 mL of Phenol-Chloroform(25:24) were added and vortexed for 10 min atRT. ... GoTaq Hot StartPolymerase (Promega, Madison, WI), 200 nM eachof forward and reverse primers, and gDNA ofBY4741 or synV (yXZX512, version -
The discovery of artemisinin (qinghaosu) and gifts from Chinese…
https://haseloff.plantsci.cam.ac.uk/resources/Part2SynBio_refs/Lecture-2/Tu2015.pdf14 Aug 2023: More than 380 extracts obtained from 200 Chinese herbs were evaluated against a mouse model of malaria. ... Chin. Tradit. Herbal Drugs 16, 200–201 (1985). 13. Wu, C.M. & Tu, Y.Y. -
Droplet-based microfluidic analysis and screening of single plant…
https://haseloff.plantsci.cam.ac.uk/resources/LabPapers/Yu2018.pdf14 Aug 2023: The tubes were. shaken at room temperature for 20 min at 200 rpm. ... high-voltage power supply. The resulting electrode pulse (200 V) deformed YFP-positive. -
BioImaging346.qxd
https://haseloff.plantsci.cam.ac.uk/resources/LabPapers/Haseloff2003a.pdf14 Aug 2023: Herethe confocal laser-scanning microscope proves its worth.High-resolution optical sections can be collected deep intosuch cleared samples to distances of greater than 200 µm. -
Programmed hierarchical patterning of bacterial populations
https://haseloff.plantsci.cam.ac.uk/resources/LabPapers/Boehm2018.pdf14 Aug 2023: Max corrected fluorescence intensity [RFU]. CFP YFP RFP. pCRB DRT7VTPLux500CFP 7000 1000 9 9 100 20pCRB DRT7VTPLux500YFP 1200 200 5000 1000 150 20pCRB DRT7VTPLux500 5000 1000 5000 1000 11,000 3000. ... loaded in a final volume of 200 μL per well onto
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