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  2. News - Cambs 24

    https://www2.mrc-lmb.cam.ac.uk/archive/articles/Road_named_after_legendary_scientist.pdf
    1 Feb 2013: Copyright 2013 Archant Regional Ltd. All rights reserved. News - Cambs 24 http://www.cambs24.co.uk/news/cambridge_road_to_be_na.
  3. Bacterial chromosome segregation: structure andDNA binding of the Soj …

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/soj_embo2005.pdf
    30 Jan 2005: The EMBO Journal VOL 24 | NO 2 | 2005 & 2005 European Molecular Biology Organization. ... The EMBO Journal VOL 24 | NO 2 | 2005 & 2005 European Molecular Biology Organization272.
  4. Pi-PEG Screen 1.5 disclaimer

    https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB19.pdf
    28 Sep 2022: 6.45 % 1-23 PEG 600 25.65 % BICINE 75 mM 8.4 PEG 2000 MME 6.45 % 1-24 PEG 1000 22.5 % BICINE 75 mM 8.8 PEG 3000 ... 8 PEG 3000 12.9 % 1-35 PEG 200 24.15 % BICINE 75 mM 8.4 PEG 4000 10.65 % 1-36 PEG 300 21.45 % BICINE 75 mM 8.8
  5. Expression of selenomethionine substituted proteins innon-methionine…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/methods/SeMet%20recipe.doc.pdf
    2 Feb 2004: J Mol Biol. 1993 Jan 5;229(1):105-24. The protein is expressed in any E.
  6. Manual_CO-311 JBScreen Solubility HTS

    https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/SOLUB.pdf
    19 Mar 2018: Description of JBScreen Solubilty HTS The screen contains 24 conditions that differ by buffer chemicals and by pH. ... These 24 conditions are presented 4 times in the screen: with the buffer components alone, with 150 mM NaCl, with 5% glycerol, and with
  7. bic079a

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/DonaldsonJ.pdf
    11 Aug 2008: In cells, the Arf GTP-bind-ing and hydrolysis cycle is regulated by GEFs (guanine-nucleo-tide-exchange factors) and GAPs (GTPase-activating proteins).There are 14 GEFs and 24 GAPs ... EMBO J. 24,2244–2253. 10 Peter, B. J., Kent, H. M., Mills, I.
  8. Addit05_NeXtal pH gradient

    https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/Addit-05.pdf
    20 Nov 2020: 1 MD4 22 6.4 Sodium Cacodylate 1 MD5 23 6.6 Sodium Cacodylate 1 MD6 24 6.8 Sodium Cacodylate 1 ME1 25 7 Sodium HEPES 1 ME2 26
  9. Molecular basis for membraneremodelling and organization Deadline for …

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/F-BAR_proteins/Le%20Poster_DEUXNew.pdf
    29 Sep 2011: 24-28 September 2011. Main Topics:Membrane curvature invesicle fusion. Membrane curvature in endocytosis and vesicle budding.
  10. Addit04b_AL-SEL-DP Formated

    https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/Addit-04b.pdf
    24 Nov 2020: FOS-CHOLINE-UNSAT-11-10 31 FC-U10-11 FCU110 6.2 335.4B 12 24 1,2-Diheptanoyl-sn-glycero-3-phosphocholine 8.4 DHPC D607 1.4 481.5C ... OHES Bachem - P1105 24.3 n/aG 2 74 CYMAL-3 120 CYMAL-3 C323 30 466.5G 3 75 CYMAL-4 38 CYMAL-4 C324 7.6 480.5G 4
  11. Structure of a cholinergic cell membraneNigel Unwina,1 Edited by ...

    https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnasS22.pdf
    4 Nov 2022: The broken yellow lines identify the well-documented ribbon of δ subunit-linked receptor dimers(14, 15, 18, 24), one of which is labeled in each leaflet to indicate the subunit ... Biophys. J. 92, 3960–3967 (2007). 24. H. W. Chang, E. Bock, Molecular
  12. Structure of a Bacterial Dynamin-like Protein Lipid Tube Provides a…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/cell%202009%20dynamin.pdf
    19 Dec 2009: ell 139, 1342–1352, December 24, 2009 ª2009 Elsevier Inc. 1347. A B. ... at either end. ell 139, 1342–1352, December 24, 2009 ª2009 Elsevier Inc.
  13. JCSG+ Screen

    https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB12.pdf
    2 Oct 2014: Section D: Conditions (16 -18) and (22 - 24) supplements the CSS-I with other PEGS mixed with KSCN. • ... 24 0.8 M Na formate 8% PEG 20,000 8% PEG 500 MME.
  14. S413_6a 39..44

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MreB%20Nature.pdf
    30 Aug 2001: 51.1 Å. IA. IBIIB. IIA. F-actin MreB. 55 Å. 1. 24. ... Interestingly, the rened model ofF-actin23,24 is less similar to our crystal structure than the initialunrened model, in which the crystal structure of G-actin incomplex with DNase I was
  15. 1 Clear StrategyTM Screen I MD1-14 A 6 × ...

    https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB10.pdf
    2 Oct 2014: MD1-14 is presented as a 24 x 10† mL stock conditions 2 x 10 mL of 5 different pH’d buffers. ... 24 0.8 M Na formate 8% PEG 20,000 8% PEG 500 MME.
  16. IL-17 is a neuromodulator of Caenorhabditis elegans sensory responses

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Nature_2017.pdf
    13 Jan 2017: Genetics 159, 133–145 (2001). 24. Gaffen, S. L. Recent advances in the IL-17 cytokine family. ... Optogenetics. L4 animals expressing ChR2 in RMG27 were picked 24 h before the assay and divided into two groups.
  17. MD1-98 The LMB Screen

    https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB21.pdf
    15 Jul 2016: Tris propane 7.11-22 24 % v/v Ethanol 0.1 M HEPES 7.8 0.04 M Magnesium chloride hexahydrate1-23 17.5 % v/v Ethanol1-24 1.3 M ... M Sodium chloride1-43 15 % w/v PEG 3350 0.1 M MES 6.21-44 24 % w/v PEG 3350 0.05 M HEPES 6.8 0.15 M Sodium
  18. Phosphoinositide-Specific Phospholipase Cδ1 Activity toward Micellar…

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9287165.pdf
    20 Apr 2002: diC7PC (8 mM) no significant activation observed 0.85( 0.06 24.7( 2.9(1.7( 0.2). ... 132) PI-PLCδ1 and at 24-45 C for full-length PI-PLCδ1 (Figure 7A,B).
  19. MD1-46 Morpheus

    https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB23.pdf
    4 Aug 2021: 1-21 B9 - - - 0.24 M Ethyleneglycol 0.1 M Buffer System 2 7.5 30 % Precipitant Mix 2. ... 1-24 B12 0.8 % Alkaloids 0.12 M Ethyleneglycol 0.1 M Buffer System 3 8.5 30 % Precipitant Mix 1.
  20. JCSG+ Screen

    https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB11.pdf
    2 Oct 2014: 24 0.8 M Na formate 8% PEG 20,000 8% PEG 500 MME. ... Section D: Conditions (16 -18) and (22 - 24) supplements the CSS-I with other PEGS mixed with KSCN. •
  21. MD1-118 Hippocrates additive screen

    https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/Addit-03.pdf
    9 Jul 2018: D3 21 1.5 % Caffeic acid †D4 22 1.5 % D-()-Quinic acidD5 23 1.5 % Gallic acid monohydrate†D6 24 1.5 % N-vanillylnonanamide (capsaicin synthetic)†E1 25 1.5 %
  22. The Journal of Cell Biology © The Rockefeller University ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/epsinR/sedequil.pdf
    16 Jan 2003: Afteran initial scan, the centrifuge was over-speeded at 24,000 rev/min for 6 h, to reduce the time taken to reach equilibrium (Van Holde and Baldwin, 1958),and then ... the speed was reduced and another scan was taken, followed by further scans at
  23. Structural and Mechanistic Comparison of Prokaryotic and Eukaryotic…

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9466937.pdf
    15 Jan 1998: 139 (E) 394 412 (Ta3) 2.64 (9)4 490 495 (Ta4) 5 6 205 222 (F) 525 534 (Ta5) 2.24 (5)60 536 545 (Ta50) 7 243 263 (G) ... 24%. Despite this relatively weak con-servation both enzymes share a very similar three-dimensional structure (Moser et al., 1997).
  24. MD1-91, MD1-92 and MD1-92-FX Morpheus II 10 mL, HT-96 and FX-96…

    https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB20.pdf
    9 Jul 2018: L-rhamnose monohydrate Monosaccharide RAM 43. DL-threonine Amino-acid DTH; THR 23;n/a. DL-histidine, HCl, H20 Amino-acid DHI; HIS 24; n/a. ... v Precipitant Mix 61-23 B11 2 mM Divalents II 0.1 M Buffer System 6 8.5 30 % v/v Precipitant Mix 71-24 B12 2 mM
  25. MD1-116-117 Morpheus III-Morpheus III HT-96

    https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB22.pdf
    9 Jul 2018: 3 8.5 30 % Precipitant Mix 21-23 B11 1.5 % Vitamins mix 0.1 M Buffer System 3 8.5 30 % Precipitant Mix 31-24 B12 1.5 % Vitamins mix ... 8.5 30 % Precipitant Mix 32-24 F12 0.6 % Antibiotics mix 0.1 M Buffer System 3 8.5 37.5 % Precipitant Mix 42-25 G1 1.2
  26. Impress

    https://www2.mrc-lmb.cam.ac.uk/coot/ephemera/beyond-coloured-lines.pdf
    28 Sep 2020: Aids). 23 / 50. Screenshots. 24 / 50. Generic Objects. 25 / 50. ... Slide 24. Slide 25. Slide 26. Slide 27. Slide 28. Slide 29.
  27. MD1-46, MD1-47 and MD1-47-FX Morpheus 10 mL, HT-96 and FX-96…

    https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB17.pdf
    9 Jul 2018: 5 30 % v/v Precipitant Mix 31-24 B12 0.09 M Halogens 0.1 M Buffer System 3 8.5 37.5 % v/v Precipitant Mix 41-25 C1 ... 0.12 M Monosaccharides 0.1 M Buffer System 3 8.5 30 % v/v Precipitant Mix 32-24 F12 0.12 M Monosaccharides 0.1 M Buffer System 3
  28. MD1-93 the Morpheus Additive Screen

    https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/Addit-01.pdf
    5 Jul 2018: L-rhamnose monohydrate Monosaccharide RAM 43. DL-threonine Amino-acid DTH; THR 23;n/a. DL-histidine, HCl, H20 Amino-acid DHI; HIS 24; n/a.
  29. The Journal of Cell Biology © The Rockefeller University ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/sedequil.pdf
    16 Jan 2003: Afteran initial scan, the centrifuge was over-speeded at 24,000 rev/min for 6 h, to reduce the time taken to reach equilibrium (Van Holde and Baldwin, 1958),and then ... the speed was reduced and another scan was taken, followed by further scans at
  30. 2-126 Formulations

    https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB05.pdf
    2 Oct 2014: Tube Number pH. 1. 7.12. 7.23. 6.24. 6.75. 5.86. 6.97. 5.18.
  31. doi:10.1016/j.cub.2004.09.077

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Snx1PeteCullen2004/Snx1PeteCullen2004.pdf
    23 Nov 2004: Curr. Opin.Cell Biol. 14, 454–462. 24. Seaman, M.N.J., Marcusson, E.G., Cereghino, J.L., and Emr,S.D.
  32. Sup Fig 1

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/YopJSup.pdf
    11 Nov 2008: 24 h after transfection cells were serum-starved for 16 h and then stimulated with 20 ng/ml TNF-α. ... 24. 26. 28. 30. 32. 34. 36. 38. 40. 42. inte.
  33. Microscopy, 2021, 1–6doi:https://doi.org/10.1093/jmicro/dfab023…

    https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/mic21.pdf
    26 Jul 2021: acetylcholine receptor clusters in C2C12 myotubes. J. Lipid. Res. 47:2121–2123. 24.
  34. Crystal Screen Lite™ is a complete reagent kit designed ...

    https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB06b.pdf
    26 Jun 2015: Please e-mail, fax, or telephone your request to Hampton Research. Fax and e-mail Technical Support are available 24 hours a day. ... 24. 0.2 M Calcium chloride dihydrate, 0.1 M Sodium acetate trihydrate pH 4.6, 10% v/v 2-Propanol.
  35. Filament structure and subcellular organization of the bacterial…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Liu_PNAS_crescentin_2024.pdf
    28 Apr 2024: This is reminiscent of eukaryotic intermediate filaments (IFs), which are also known to be non- polar (21–24). ... mass spectrometry anal-yses of IF protein oligomers, and recent cryo- EM analyses (21–24,.
  36. MD1-118 Hippocrates additive screen

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/LMB%20plates/Addit-03.pdf
    13 Oct 2020: D3 21 1.5 % Caffeic acid †D4 22 1.5 % D-()-Quinic acidD5 23 1.5 % Gallic acid monohydrate†D6 24 1.5 % N-vanillylnonanamide (capsaicin synthetic)†E1 25 1.5 %
  37. Crystal structure of the SOS cell division inhibitorSulA and ...

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/SulA%20PNAS%202003.pdf
    13 Jun 2003: To whom correspondence should be addressed. E-mail: jyl@mrc-lmb.cam.ac.uk. www.pnas.orgcgidoi10.1073pnas.1330742100 PNAS June 24, 2003 vol. ... Lowe, J. (1999) Struct.Folding Des. 7, 1181–1187. 24. Van Duyne, G.
  38. 1202.tif

    https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jcb84.pdf
    16 Jun 2006: Science (Wash. DC). 208:1454-1457. 24. Ross, M. J., M. W. Klymkowsky, D. ... J. Ultrastruct. R e s 24:454-564. 27. Ziogsheim, H.-P., D.-C. Neugehauer, F.
  39. Tutorial 4: Statistical Theory;Why is the Gaussian Distribution so ...

    https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_statistics_course_2014/tutorials/statistical_theory_why_is_the_gaussian_distribution_so_popular_answers.pdf
    25 Mar 2014: If X Bin(100, 0.4) then E(X) = 40 and V ar(X) = 24. ... 8. pnorm(35,40,sqrt(24)). Answer: 0.154 (3sf). Statistical Theory; Why is the Gaussian Distribution so popular?
  40. se060101051p

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/ford01.pdf
    3 Dec 2004: The COOH-termi-nal domains of these proteins contain clath-rin-binding motifs (3, 24 ), as well as Asp-Pro-Phe (DPF)-like a- and b-adaptin–. ... De Camilli, A. T.Brunger, J. Cell Biol. 149, 537 (2000). 24.
  41. doi:10.1016/j.jmb.2004.05.031

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/zapa%202004.pdf
    16 Aug 2004: In vitro, FtsZ isextremely versatile, forming protofilaments, thickfilaments, sheets,21,22 helical tubes23,24 and asters.25. ... FEMS Microbiol. Rev. 24,531–548. 13. Rothfield, L., Justice, S. & Garcia-Lara, J.(1999).
  42. Proteins containing photosynthetic reaction centre domains modulate…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Nussbaum_Nat_micro_2024.pdf
    1 Mar 2024: A short. 00:00 h 03:00 h 06:00 h 09:00 h 24:00 h. ... 00:00 h 03:00 h 06:00 h 09:00 h 24:00 h. H26.
  43. mmi_3991.fm

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/ftsn2004.pdf
    15 Apr 2004: 22|i-j|24) 78 Weak (0–5.0 Å) 183Long range (|i – j| > 4) 163Total 554 (=7.1 per residue)Statistics for 45 accepted structures:. ... J ApplCrystallogr 24: 946–950. Kuroda, A., Asami, Y., and Sekiguchi, J.
  44. MinCD cell division proteins form alternating copolymeric cytomotive…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/ncomms6341.pdf
    6 Dec 2014: The forward primer (dg173)had 51 nt complementary sequence to the immediate upstream sequence of minoperon followed by 24 nt complementary sequence to 50-end of MinC. ... Similarly,the reverse primer (dg175) had 51 nt complementary sequence to the
  45. NPGRJ_NSMB_1158 1..8

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nsmbFtsK2006.pdf
    12 Oct 2006: coli chromosome segregation byorienting the FtsK translocase. EMBO J. 24, 3770–3780 (2005). ... J. Mol. Biol. 343, 547–557 (2004). 24. Ohnishi, T., Hishida, T., Harada, Y., Iwasaki, H. &
  46. MergedFile

    https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/iucrj17.pdf
    24 May 2017: L., Graf-Meyer, A., Vogel, H. & Stahlberg, H. (2016). Structure, 24,165–170.
  47. struct1345mmc1

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/FCH02F-BAR_SUP07.pdf
    2 Jun 2007: After the overspeeding, interference scans were taken at 24 h intervals, and, when.
  48. Cryo‐EM structure of the full‐length Lon protease from Thermus…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Lon%20Coscia%202021%20FEBS.pdf
    14 Nov 2021: 24]. All subsequent image-processing steps were performed. using single-particle reconstruction methods in RELION. ... improved cryo-electron microscopy. Nat Methods 14,. 331–332.24 Zhang K (2016) Gctf: Real-time CTF determination.
  49. LETTERS A bacterial dynamin-like proteinHarry H. Low1 & Jan ...

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nature%20BDLP%202006.pdf
    28 Nov 2006: Diffraction data 3.0 Å, all data 3.1 Å, all dataR factor, Rfree 0.24 (0.34), 0.28 (0.39) 0.22 (0.35), 0.27 (0.36)B ... Nature 440, 101–104 (2006). 24. Hales, K. G. & Fuller, M.
  50. Acetylation of MEK2 and I{kappa}B kinase (IKK) activation loop…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/YopJ.pdf
    28 Nov 2006: CELL. BIO. LOG. Y. radation of -catenin (24). It is thus possible that YopJ-relatedAvrA may also possess an acetyl transferase activity allowing itto inactivate IKK thereby exerting its effect on ... Gastrointest Liver Physiol 287:G220 –227.24.
  51. INVITED REVIEW Mechanosensory molecules and circuits in C. elegans ...

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/2015-2.pdf
    10 Dec 2014: These mayinclude proposed extracellular tethers such as MEC-1 orMEC-9 [24] or alternatively may involve other, as yet uniden-tified sensor proteins. ... Nature 349(6310):588–593. doi:10.1038/349588a0. 24. Du H, Gu G, William CM, Chalfie M (1996)

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