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2 Jun 2004: glass coverslips in 24-well plates were infected with trans-fected parasites and were processed for IF 20 –24 h post-transfection asdescribed (32). ... 23 Golgi Type I TM 90BAPVSVG 24 Golgi Type I TM 905-poly(L)ext 26 Golgi ND ND.

24 Sep 2004: Received for publication, January 5, 2004, and in revised form, March 22, 2004Published, JBC Papers in Press, March 24, 2004, DOI 10.1074/jbc.M400023200. ... 2002) Biochim. Biophys. Acta 1579, 219 –224. 24. Bishop, N., Horman, A., and Woodman, P.

15 Jan 2004: PrP 22-24 was detected at 112 days and130 days using the cold PK digestion protocol (Fig. ... 5A, B,and D). Mutant HuPrP 22-24 was found in Tg69 mice at 200days of age (Fig.

29 Dec 2004: Vol. 265, No. 24, Issue of August 25, pp. 14364-14370,199O Printed in V. ... K eq was determined at 0 “C! while Kd was obtained at 24 “C.

24 Sep 2004: Protein atoms 4404 4164 4138Waters 265 324 259Rcrystd 20.2 19.9 22.6Rfreed (% data used) 24.1 (5) 24.3 (5) 26.7 (5)r.m.s.d. ... inosi-in 1 l sitting drops over reservoirs of 24-well plates (Hampton).

1 Nov 2004: Multiplicity (last shell) 14.1 (14.1) 14.3 (14.2) 11.6 (11.6) 7.2 (7.2) 6.9 (3.9) 24.9 (24.0)I/ (last shell)

8 Nov 2004: After 18–24 h, cells were harvested for parallelluciferase measurements (using a Luciferase Reporter Kit fromRoche) and immunoblotting. ... Biochemical analysesExperiments analyzing PrP constructs were performed between 18and 24 h after transfection

21 Dec 2004: Between 5000 and 10,000 cells were plated per well of a 96-well platebetween 24 and 36 h before transfection, at which time they were 40 – 80%confluent. ... Unless other-wise noted in the figure legend, the cells were analyzed 18 –24 h after

23 Nov 2004: Transferrin uptake and immunofluorescenceCos 7 cells were transfected using GeneJuice according to manufacturers instructions,plated onto polylysine coated coverslips 24 hours post transfection and assayed fortransferrin uptake 48 hours post

2 Feb 2004: Membrane transport between compartments in eukary-otic cells requires proteins that allow the budding andSCISSION of nascent cargo vesicles from one compartmentand their targeting and fusion with another. Just asSNARES are proposed to be essential