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DOI: 10.1126/science.1256917, 963 (2014);346 Science et al.Thomas G.…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Science-2014-Gligoris-963-7.pdf21 Nov 2014: Biol. 16, 1998–2008. (2006).12. B. Hu et al., Curr. Biol. 21, 12–24 (2011).13. ... F. Uhlmann et al., Nature 400, 37–42 (1999).23. S. B. Buonomo et al., Cell 103, 387–398 (2000).24. -
Amphiphysin is necessaryfor organization of the…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/DroxAmph.pdf9 Nov 2001: Amphiphysin is necessaryfor organization of the excitation–contraction coupling machineryof muscles, but not for synaptic vesicleendocytosis in DrosophilaAzam Razzaq,1,2 Iain M. Robinson,1 Harvey T. McMahon,3 Jeremy N. Skepper,4 Ya Su,1,7. Andrew C -
Amos&Schlieper.indd
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Amos%26Schlieper.pdf16 Nov 2005: MICROTUBULES AND MAPs. By LINDA A. AMOS AND DANIEL SCHLIEPERMRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK. I. IntroductionII. Microtubule Structure A. Subunit Lattices B. Atomic Structure of the Protofilament C. Atomic -
mk069902063p
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Fitting.MBC.pdf26 May 1999: J. Appl. Crystallogr. 24, 946 –950. Kull, F.J., Sablin, E.P., Lau, R., Fletterick, R.J., and Vale, R.D. -
Qrb1300006 1..40
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/qrb13.pdf30 Jul 2013: Transition to open-channel form 23. 5.1. Introduction 23. 5.2. Spray-freeze-trapping experiments 24. -
REPORT◥ PROTEOSTASIS UBE2O is a quality control factor fororphans ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017b.pdf4 Aug 2017: In most cellsunder normal conditions, orphan proteins prob-ably represent the predominant clients for qua-lity control given the large number of abundantmultiprotein complexes (23, 24), the challengesof precisely matching expression ... N. J. Krogan et al -
doi:10.1016/j.coph.2005.03.002
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/15963759.pdf12 Nov 2006: Mol Cell 2005, 18:13-24. A convincing and important description of the first selective S473-PKBphosphatase. ... 24. Welch HC, Coadwell WJ, Stephens LR, Hawkins PT:Phosphoinositide 3-kinase-dependent activation of Rac.FEBS Lett 2003, 546:93-97. -
www.sciencemag.org/cgi/content/full/1164346/DC1 Supporting Online…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Salje%20et%20al%20Science%202009%20Suppl.pdf15 Dec 2008: at a pixel size of 0.24 to 0.42 nm. Electron cryomicroscopy imaging was performed at 300 kV either on an. ... 23. Figure S4 FPlasmid-free control. 24. A B. C D. Figure S5 low-copy number R1 plasmid (pKG491). -
Interaction of tau protein with the dynactincomplex Enrico…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/EMBOJ2007-Magnani-Fan.pdf15 Oct 2007: Annu Rev Neurosci 24: 1121–1159. Ljwe J, Amos LA (1998) Crystal structure of the bacterial cell-division protein FtsZ. ... J Neurosci 24:4657–4667. Tau interaction with dynactin p150E Magnani et al. -
doi:10.1016/j.jmb.2007.08.056
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Oliva_FtsZ_JMB_2007.pdf11 Oct 2007: insta-bility.23,24 It is not known whether the Z ring hasa direct mechanical role in cell division or merelyserves as a scaffold for the recruitment of other celldivision ... containing 0.1 M Mes–NaOH (pH 6.0), 0.24 M (NH4)2SO4,11% (w/v) PEG8000 and -
DOI: 10.1126/science.1165322 , 1710 (2008); 322Science et al.Rachel…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/19008417.pdf18 Dec 2008: 80, 7625 (2006).24. We thank O. Perisic and I. Duggin for advice and helpful. -
201010176 1..6
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2010-Aylett-TubZ.pdf21 Oct 2010: Edited by Stephen C. Harrison, Harvard Medical School, Boston, MA, and approved September 24, 2010 (received for review July 13, 2010). -
Four-stranded mini microtubules formed by Prosthecobacter BtubAB show …
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2017-Deng-E5950-8.pdf29 Dec 2017: For BtubAB filament reconstruction, 1,394 movies were usedfrom two separate 24-h microscope sessions. ... S1). For BtubABC filament reconstruction, 6,105 movies were used from fourseparate 24-h microscope sessions. -
The Structure of a Tunicate C-type Lectin from Polyandrocarpa…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/10398588.pdf7 Jul 1999: Joint CCP4 and ESF-EACBM Newsletter for Protein Crystallography, 33, 22-24. Gabius, H. ... Biophys.Biomol. Struct. 24, 551-577. Sambrook, J., Fritsch, E. F. & Maniatis, T. -
Crystal Structure of a Calcium-Phospholipid Binding Domainfrom…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9430701.pdf20 Apr 2002: Crystal Structure of a Calcium-Phospholipid Binding Domainfrom Cytosolic Phospholipase A2. (Received for publication, September 24, 1997). ... 1991) J. Appl. Crystallogr. 24, 946 –95072. Nicholls, A. (1992) GRASP: Graphical Representation and Analysis -
elifesciences.org van den Ent et al. eLife 2014;3:e02634. DOI: ...
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/mrebdouble2014.pdf6 Jun 2014: elifesciences.org. van den Ent et al. eLife 2014;3:e02634. DOI: 10.7554/eLife.02634 1 of 22. Bacterial actin MreB forms antiparallel double filamentsFusinita van den Ent1†, Thierry Izoré1†, Tanmay AM Bharat1, Christopher M Johnson2, Jan Löwe1. -
Influence of lipid bilayer on the structure of the muscle-type…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnasS24.pdf30 Apr 2024: in cell- derived membrane vesicles (24), or by in situ cryoelectron tomography (25). ... Electron Microscopy Data Bank https://www.ebi.ac.uk/emdb/EMD-18845. Deposited 6 November 2023. 24. N.Unwin, Influence of lipid bilayer on structure of acetylcholine -
JCSG+ Screen
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB15.pdf2 Oct 2014: 0. 4. 8. 12. 16. 20. 24. Coun. ts. Detergents used for membrane protein crystallisation. ... 24. 0.1 M sodium chloride. 0.1 M magnesium chloride. 0.1 M CAPSO. -
Journal of Visualized Experiments www.jove.com Copyright © 2018…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/JOVE2018.pdf19 Apr 2018: JBS6 JenaBioScience CS-106L 24 Incomplete factorial based on AmS (pH 4.6-8.5). ... JBS7 JenaBioScience CS-107L 24 Incomplete factorial based on MPD (pH 4.6-8.5). -
PII: S0014-5793(01)02216-5
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MinD%20FEBS.pdf5 Mar 2001: 1990) Annu.Rev. Genet. 24, 249274. [3] Lutkenhaus, J. and Addinall, S.G. ... Appl. Crystallogr. 24, 946950.[34] Merritt, E.A. and Bacon, D.J. (1997) in : Macromolecular Crys-. -
�������� ��� � ���� �� �� ���� ��� ������ ...
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/FtsA%20EMBO%20.pdf4 Oct 2000: E#N# 04412 (B<-! A # # " 3 16G7H#. # >$$I# ># 3 J#3# 04492 ' ( ' ) % % # ) &. 2'99G997#. $# B # O (# 0442 ( (- $ $ 6H $ # # / 0/ 24 64HG647#. (#-# -
doi:10.1016/j.pbiomolbio.2004.07.009
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/stock2005robot.pdf19 Jan 2005: Quik phosphate grid screen 24 Hampton Research. NaCl grid screen 24 Hampton Research. ... The platform also accommodates most commercially availablecrystallisation plates (24-well Linbro (Hampton Research, #HR3-110), 24-well Cryschem(Hampton Research, -
1 CURRICULUM VITAE Venki Ramakrishnan Nationality U.S. (since 1985)…
https://www2.mrc-lmb.cam.ac.uk/groups/ribo/download/ramakrishnan_cv_4.pdf4 Jan 2024: Nat Struct Mol Biol 24, 866-869. N. R. James, A. Brown, Y. ... 123, 24-34. Gerchman, S.E. and Ramakrishnan, V. (1987). Chromatin higher-order structure studied by neutron scattering and scanning transmission electron microscopy. -
KWRM_A_992668_O
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/2015-3.pdf20 Oct 2015: Submitted: 11/06/2014. Revised: 11/21/2014. Accepted: 11/24/2014. http://dx.doi.org/10.4161/21624054.2014.992668This is an Open Access article distributed under theterms of the ... Molecular anatomy of a trafficking organelle.Cell 2006; 127:831-46; -
Nature © Macmillan Publishers Ltd 1998 8 30. Fan, ...
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/FtsZ.Nature.pdf9 Jan 1998: Biochem. 24, 255–302 (1995). 22. Leslie, A. G. W. Recent changes to the MOSFLM package for processing film and image plate data (SERCLaboratory, Daresbury, Warrington, UK, 1991). ... 23. The CCP4 suite: Programs for protein crystallography. Acta -
PII: 0304-3991(84)90197-9
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/ultram84.pdf26 Oct 2008: The capacity of the Dewar is sufficient to give a running time of about 24 h under these conditions. -
A Large Particle Associated with the Perimeter of the ...
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jcb82.pdf5 Jul 2010: Best preserved porecomplexes show evidence of 16- and 24-fold harmonics inaddition to the basic eightfold harmonic (Fig. ... J. CellBiol. 52:338-354. 24. Paine, P. L. , L, C. Moore, and S. -
Cryo-EM reconstruction of AlfA from Bacillus subtilis reveals the…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/AlfA2018.pdf20 Mar 2018: The subunits are staggered in a left-handed, double-heli-cal, parallel (polar) filament, with a rise of 24.9 Å and a 156.5 twist. ... Science 338:1334–1337. 24. Schumacher MA, et al. (2007) Segrosome structure revealed by a complex of ParRwith -
53091 361..366
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/McMahonE.pdf3 Dec 2004: 24). Thus our experiments point to a pioneering role forepsin in vesicle budding as it provides both a driving force and a linkbetween membrane invagination, clathrin polymerization and AP2complex recruitment. ... beta 2-adaptin appendage domain. EMBO J. -
Structure of bacterial tubulin BtubA�B:Evidence for horizontal gene…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/btubab%202005.pdf21 Jun 2005: BtubAB form the same het-erodimer as tubulin (24, 25). The protofilament axis isvertical. ... 1993) Micron 24, 465– 471. 10. Petroni, G., Spring, S., Schleifer, K.-H., Verni, F. & -
nature structural biology . volume 5 number 6 . june 1998
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nsb1998.pdf14 Jan 2002: 24.Chook, Y.M., Gray, J.V., Ke, H. & Lipscomp, W.N. The monofunctional chorismate mutase from Bacillus subtilis: structure determination of chorismate mutase and its complexes with a transition state ... J. Appl. Cryst. 24, 946-950 (1991). 39.Merrit, E.A. -
The Jour nal o f Cel l Bio logy ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/26_Snapp_EL_JCB_2003.pdf16 Oct 2003: The. Jour. nal o. f Cel. l Bio. logy. JCB. Article. The Journal of Cell Biology, Volume 163, Number 2, October 27, 2003 257–269http://www.jcb.org/cgi/doi/10.1083/jcb.200306020 257. Formation of stacked ER cisternae by low affinity protein -
Activation of Xer-recombination at dif: structural basis of the…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/srep33357.pdf30 Sep 2016: identification of a potential N-terminal targeting domain. J Bacteriol 180, 1296–1304 (1998).24. ... EMBO J 24,. 3770–3780, doi: 10.1038/sj.emboj.7600835 (2005).29. Levy, O. et al. -
Organization of spines on the dendritesof Purkinje cellsJohn O’Brien…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnas06.pdf25 Jul 2006: 2004) Nat. Rev. 5, 24 –34.4. Matus, A. (2000) Science 290, 754 –758.5. ... T., Wong, R. O. L. & Lichtman, J. W. (2000) Neuron 27, 219 –225.24. -
se289900215p
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/strucrev.pdf6 Jul 1999: Adaptors EM, rotary shadow and deep-etch; APcomplexes (13). ;25 (2D). Crystallography; AP2 m2 residues 158 to435 (24). ... 2.7 Phe174, Arg423 (24) YxxØ endocytosis signals. Crystallography; AP2 COOH-terminala-adaptin appendage domain residues701 to 938 -
Structure and superorganization of acetylcholinereceptor–rapsyn…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnas13.pdf3 Jul 2013: 20%of the receptors showed restricted diffusion in small domains of50 nm, whereas the majority of the receptors were immobile (24).Confocal microscopy of overexpressed rapsyn in nonmuscle. ... The cross-linking of receptors by rapsyn,therefore, seems to -
se060101051p
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/AP180/Ap180_st.pdf3 Dec 2004: The COOH-termi-nal domains of these proteins contain clath-rin-binding motifs (3, 24 ), as well as Asp-Pro-Phe (DPF)-like a- and b-adaptin–. ... De Camilli, A. T.Brunger, J. Cell Biol. 149, 537 (2000). 24. -
www.landesbioscience.com Autophagy 805 Autophagy 6:6, 805-807; August …
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/20574157.pdf2 Aug 2010: Submitted: 05/24/10. Revised: 06/03/10. Accepted: 06/03/10. Previously published online: www.landesbioscience.com/journals/autophagy/article/12577Correspondence to: Roger L. -
Sensory Neurons Arouse C. elegans Locomotion via Both Glutamate and…
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/2015-4.pdf30 Jun 2015: For this experiment, we utilized transgenicanimals that express rat TRPV1 capsaicin receptors in ASH neurons [24]. ... In these animals,capsaicin treatment evokes ASH-mediated avoidance behaviors [24]. A 5-hour capsaicin treat-ment had little effect on L4 -
Bacterial actin MreB assembles in complex with cell shape protein RodZ
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embo2010RodZMreB.pdf17 Feb 2010: The crystals were cryoprotected with mother liquorcontaining 24.8% PEG400 and flash frozen in liquid nitrogen. ... The plate was incubated at 301Cand imaged after 24 h. DrodZ complementation assays and microscopy. -
PII: 0896-6273(95)90037-3
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/neuron95.pdf4 May 2004: Number of Receptors Phase Residual" Weight in Final. scFv35 Film number 4280 3560 23.5 0.18 Film number 4282 4360 24.2 0.23 Film number 4295 4820 16.1 ... Biochemistry 24, 1212- 1221. -
se060101047p
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Itoh%20et%20al.pdf23 Nov 2004: Quantitativerepresentations of co-sedimentation assay data of GST-ENTH domains from (D) epsin and (E)AP180 (24) with 0.5% of the indicated phospholipids in the liposomes. ... X-ray Crystallography and NMR (Yale Univ. Press,New Haven, CT, 1993). 24. -
mc4604.qxd
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/current%20opinion%20micro%202001.pdf12 Nov 2001: Mol Microbiol 1996, 22:231-237. 19. Margolin W: Themes and variations in prokaryotic cell division.FEMS Microbiol Rev 2000, 24:531-548. ... J Cell Biol 2000, 151:F19-F21.See annotation to [24•]. 24. Kiessling J, Kruse S, Rensing SA, Harter K, Decker EL, -
Supplementary Figure 1Lysophosphatidic acid acyl transferase (LPAAT)…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndoSupFigs.pdf4 Jan 2006: 35. 24. 21. Bead. s pos. t clea. vage. Purif. ied cl. -
Collaborative protein filaments
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/ghosal_embo_2015.pdf4 Jan 2016: Mol. Microbiol 24: 905 – 915. van den Ent F, Izore T, Bharat TA, Johnson CM, Löwe J (2014) Bacterial actin. ... EMBO J 24: 270 – 282. Lesterlin C, Ball G, Schermelleh L, Sherratt DJ (2014) RecA bundles mediate. -
BAR Domains as Sensors ofMembrane Curvature: TheAmphiphysin BAR…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/PeterBARdomains.pdf30 Jan 2004: recruit clathrin to membranes. Mammalian am-phiphysin2 has several clathrin-binding sites (24–26), and we found that the wild-type, but notmut1, efficiently recruited clathrin to liposomes(fig. ... It is interesting to note that thestructure of the -
Activation of GCN2 by the ribosomal P-stalk
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/PNAS_2019.pdf7 Mar 2019: Yeast GCN2 forms aconstitutive dimer even in the absence of activation, principallythrough the CTD (24, 25). ... This lack of clarity was further con-founded by a more recent report that, unlike yeast, mouseGCN2 does not form a stable complex that -
1256917combinedsupplement
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Gligoris-SM.pdf14 Nov 2014: Curr. Biol. 21, 12–24 (2011). Medline doi:10.1016/j.cub.2010.12.004. 13. E. Unal, J. ... Cell 103, 387–398 (2000). Medline doi:10.1016/S0092-8674(00)00131-8. 24. A. Losada, M. Hirano, T. -
st7a11.qxd
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MukB%20Structure%201999.pdf10 Sep 1999: 15, 430-434. 24. Akhmedov, A.T., Frei, C., Tsai-Pflugfelder, M., Kemper, B., Gasser,S.M. & ... J. Appl. Crystallogr.24, 946-950. 33. Barton, G.J. (1993). ALSCRIPT: a tool to format multiple sequencealignments. -
The MORPHEUS protein crystallization screen
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/morpheus%202009.pdf12 Nov 2009: 1991). J. Appl. Cryst. 24, 409–411.Joerger, A. C., Ang, H. C. & ... m, n. Ran Ran GTPase 10.0 24.5 LMB, Danguole Ciziene Stewart et al.
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