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    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/leoa2014.pdf
    3 Sep 2014: replacing the leoˤ leoB and leoAB genes with a chloramphenicolresistance cassette as described [24]. ... 24. Datsenko KA, Wanner BL (2000) One-step inactivation of chromosomal genes.

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