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News - Cambs 24
https://www2.mrc-lmb.cam.ac.uk/archive/articles/Road_named_after_legendary_scientist.pdf1 Feb 2013: Copyright 2013 Archant Regional Ltd. All rights reserved. News - Cambs 24 http://www.cambs24.co.uk/news/cambridge_road_to_be_na. -
bic079a
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/DonaldsonJ.pdf11 Aug 2008: In cells, the Arf GTP-bind-ing and hydrolysis cycle is regulated by GEFs (guanine-nucleo-tide-exchange factors) and GAPs (GTPase-activating proteins).There are 14 GEFs and 24 GAPs ... EMBO J. 24,2244–2253. 10 Peter, B. J., Kent, H. M., Mills, I. -
Pi-PEG Screen 1.5 disclaimer
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB19.pdf28 Sep 2022: 6.45 % 1-23 PEG 600 25.65 % BICINE 75 mM 8.4 PEG 2000 MME 6.45 % 1-24 PEG 1000 22.5 % BICINE 75 mM 8.8 PEG 3000 ... 8 PEG 3000 12.9 % 1-35 PEG 200 24.15 % BICINE 75 mM 8.4 PEG 4000 10.65 % 1-36 PEG 300 21.45 % BICINE 75 mM 8.8 -
S413_6a 39..44
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MreB%20Nature.pdf30 Aug 2001: 51.1 Å. IA. IBIIB. IIA. F-actin MreB. 55 Å. 1. 24. ... Interestingly, the rened model ofF-actin23,24 is less similar to our crystal structure than the initialunrened model, in which the crystal structure of G-actin incomplex with DNase I was -
mk069902063p
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Fitting.MBC.pdf26 May 1999: J. Appl. Crystallogr. 24, 946 –950. Kull, F.J., Sablin, E.P., Lau, R., Fletterick, R.J., and Vale, R.D. -
Manual_CO-311 JBScreen Solubility HTS
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/SOLUB.pdf19 Mar 2018: Description of JBScreen Solubilty HTS The screen contains 24 conditions that differ by buffer chemicals and by pH. ... These 24 conditions are presented 4 times in the screen: with the buffer components alone, with 150 mM NaCl, with 5% glycerol, and with -
doi:10.1016/j.cub.2004.09.077
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Snx1PeteCullen2004/Snx1PeteCullen2004.pdf23 Nov 2004: Curr. Opin.Cell Biol. 14, 454–462. 24. Seaman, M.N.J., Marcusson, E.G., Cereghino, J.L., and Emr,S.D. -
Addit05_NeXtal pH gradient
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/Addit-05.pdf20 Nov 2020: 1 MD4 22 6.4 Sodium Cacodylate 1 MD5 23 6.6 Sodium Cacodylate 1 MD6 24 6.8 Sodium Cacodylate 1 ME1 25 7 Sodium HEPES 1 ME2 26 -
ELIFE07975 1..21
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/94_Voorhees_RM_eLife_2015.pdf11 Aug 2015: By contrast, TMDs responsible for targeting aremarkedly more hydrophobic and between 17 to 24 residues long. -
Addit04b_AL-SEL-DP Formated
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/Addit-04b.pdf24 Nov 2020: FOS-CHOLINE-UNSAT-11-10 31 FC-U10-11 FCU110 6.2 335.4B 12 24 1,2-Diheptanoyl-sn-glycero-3-phosphocholine 8.4 DHPC D607 1.4 481.5C ... OHES Bachem - P1105 24.3 n/aG 2 74 CYMAL-3 120 CYMAL-3 C323 30 466.5G 3 75 CYMAL-4 38 CYMAL-4 C324 7.6 480.5G 4 -
Mechanisms of Dense Core Vesicle Recapture following “Kiss andRun” ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Insulin_Tsuboi_JBC2004/Insulin_TsuboiJBC2004.pdf6 Dec 2004: Received for publication, July 20, 2004, and in revised form, August 24, 2004Published, JBC Papers in Press, August 25, 2004, DOI 10.1074/jbc.M408179200. ... MIN6 Cell Culture—MIN6 -cells (24) were used between passages19 and 30 and grown in -
Tutorial 4: Statistical Theory;Why is the Gaussian Distribution so ...
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_statistics_course_2014/tutorials/statistical_theory_why_is_the_gaussian_distribution_so_popular_answers.pdf25 Mar 2014: If X Bin(100, 0.4) then E(X) = 40 and V ar(X) = 24. ... 8. pnorm(35,40,sqrt(24)). Answer: 0.154 (3sf). Statistical Theory; Why is the Gaussian Distribution so popular? -
JCSG+ Screen
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB12.pdf2 Oct 2014: Section D: Conditions (16 -18) and (22 - 24) supplements the CSS-I with other PEGS mixed with KSCN. • ... 24 0.8 M Na formate 8% PEG 20,000 8% PEG 500 MME. -
Bacterial chromosome segregation: structure andDNA binding of the Soj …
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/soj_embo2005.pdf30 Jan 2005: The EMBO Journal VOL 24 | NO 2 | 2005 & 2005 European Molecular Biology Organization. ... The EMBO Journal VOL 24 | NO 2 | 2005 & 2005 European Molecular Biology Organization272. -
1 Clear StrategyTM Screen I MD1-14 A 6 × ...
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB10.pdf2 Oct 2014: MD1-14 is presented as a 24 x 10† mL stock conditions 2 x 10 mL of 5 different pH’d buffers. ... 24 0.8 M Na formate 8% PEG 20,000 8% PEG 500 MME. -
Expression of selenomethionine substituted proteins innon-methionine…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/methods/SeMet%20recipe.doc.pdf2 Feb 2004: J Mol Biol. 1993 Jan 5;229(1):105-24. The protein is expressed in any E. -
MD1-46 Morpheus
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB23.pdf4 Aug 2021: 1-21 B9 - - - 0.24 M Ethyleneglycol 0.1 M Buffer System 2 7.5 30 % Precipitant Mix 2. ... 1-24 B12 0.8 % Alkaloids 0.12 M Ethyleneglycol 0.1 M Buffer System 3 8.5 30 % Precipitant Mix 1. -
Molecular basis for membraneremodelling and organization Deadline for …
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/F-BAR_proteins/Le%20Poster_DEUXNew.pdf29 Sep 2011: 24-28 September 2011. Main Topics:Membrane curvature invesicle fusion. Membrane curvature in endocytosis and vesicle budding. -
MD1-98 The LMB Screen
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB21.pdf15 Jul 2016: Tris propane 7.11-22 24 % v/v Ethanol 0.1 M HEPES 7.8 0.04 M Magnesium chloride hexahydrate1-23 17.5 % v/v Ethanol1-24 1.3 M ... M Sodium chloride1-43 15 % w/v PEG 3350 0.1 M MES 6.21-44 24 % w/v PEG 3350 0.05 M HEPES 6.8 0.15 M Sodium -
JCSG+ Screen
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB11.pdf2 Oct 2014: 24 0.8 M Na formate 8% PEG 20,000 8% PEG 500 MME. ... Section D: Conditions (16 -18) and (22 - 24) supplements the CSS-I with other PEGS mixed with KSCN. • -
MD1-118 Hippocrates additive screen
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/Addit-03.pdf9 Jul 2018: D3 21 1.5 % Caffeic acid †D4 22 1.5 % D-()-Quinic acidD5 23 1.5 % Gallic acid monohydrate†D6 24 1.5 % N-vanillylnonanamide (capsaicin synthetic)†E1 25 1.5 % -
MD1-91, MD1-92 and MD1-92-FX Morpheus II 10 mL, HT-96 and FX-96…
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB20.pdf9 Jul 2018: L-rhamnose monohydrate Monosaccharide RAM 43. DL-threonine Amino-acid DTH; THR 23;n/a. DL-histidine, HCl, H20 Amino-acid DHI; HIS 24; n/a. ... v Precipitant Mix 61-23 B11 2 mM Divalents II 0.1 M Buffer System 6 8.5 30 % v/v Precipitant Mix 71-24 B12 2 mM -
The Journal of Cell Biology © The Rockefeller University ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/epsinR/sedequil.pdf16 Jan 2003: Afteran initial scan, the centrifuge was over-speeded at 24,000 rev/min for 6 h, to reduce the time taken to reach equilibrium (Van Holde and Baldwin, 1958),and then ... the speed was reduced and another scan was taken, followed by further scans at -
The Jour nal o f Cel l Bio logy ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/26_Snapp_EL_JCB_2003.pdf16 Oct 2003: The. Jour. nal o. f Cel. l Bio. logy. JCB. Article. The Journal of Cell Biology, Volume 163, Number 2, October 27, 2003 257–269http://www.jcb.org/cgi/doi/10.1083/jcb.200306020 257. Formation of stacked ER cisternae by low affinity protein -
MD1-116-117 Morpheus III-Morpheus III HT-96
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB22.pdf9 Jul 2018: 3 8.5 30 % Precipitant Mix 21-23 B11 1.5 % Vitamins mix 0.1 M Buffer System 3 8.5 30 % Precipitant Mix 31-24 B12 1.5 % Vitamins mix ... 8.5 30 % Precipitant Mix 32-24 F12 0.6 % Antibiotics mix 0.1 M Buffer System 3 8.5 37.5 % Precipitant Mix 42-25 G1 1.2 -
MD1-46, MD1-47 and MD1-47-FX Morpheus 10 mL, HT-96 and FX-96…
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB17.pdf9 Jul 2018: 5 30 % v/v Precipitant Mix 31-24 B12 0.09 M Halogens 0.1 M Buffer System 3 8.5 37.5 % v/v Precipitant Mix 41-25 C1 ... 0.12 M Monosaccharides 0.1 M Buffer System 3 8.5 30 % v/v Precipitant Mix 32-24 F12 0.12 M Monosaccharides 0.1 M Buffer System 3 -
Structural and Mechanistic Comparison of Prokaryotic and Eukaryotic…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9466937.pdf15 Jan 1998: 139 (E) 394 412 (Ta3) 2.64 (9)4 490 495 (Ta4) 5 6 205 222 (F) 525 534 (Ta5) 2.24 (5)60 536 545 (Ta50) 7 243 263 (G) ... 24%. Despite this relatively weak con-servation both enzymes share a very similar three-dimensional structure (Moser et al., 1997). -
MD1-93 the Morpheus Additive Screen
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/Addit-01.pdf5 Jul 2018: L-rhamnose monohydrate Monosaccharide RAM 43. DL-threonine Amino-acid DTH; THR 23;n/a. DL-histidine, HCl, H20 Amino-acid DHI; HIS 24; n/a. -
Impress
https://www2.mrc-lmb.cam.ac.uk/coot/ephemera/beyond-coloured-lines.pdf28 Sep 2020: Aids). 23 / 50. Screenshots. 24 / 50. Generic Objects. 25 / 50. ... Slide 24. Slide 25. Slide 26. Slide 27. Slide 28. Slide 29. -
2-126 Formulations
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB05.pdf2 Oct 2014: Tube Number pH. 1. 7.12. 7.23. 6.24. 6.75. 5.86. 6.97. 5.18. -
Structural Mapping of the Catalytic Mechanism for a…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9048554.pdf20 Apr 2002: 4.6)〈I/σ(I)〉b 24.1(5.1) 15.7(4.3) 20.6(4.4) 17.2(4.9). -
Microscopy, 2021, 1–6doi:https://doi.org/10.1093/jmicro/dfab023…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/mic21.pdf26 Jul 2021: acetylcholine receptor clusters in C2C12 myotubes. J. Lipid. Res. 47:2121–2123. 24. -
The Journal of Cell Biology © The Rockefeller University ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/sedequil.pdf16 Jan 2003: Afteran initial scan, the centrifuge was over-speeded at 24,000 rev/min for 6 h, to reduce the time taken to reach equilibrium (Van Holde and Baldwin, 1958),and then ... the speed was reduced and another scan was taken, followed by further scans at -
Crystal Screen Lite™ is a complete reagent kit designed ...
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB06b.pdf26 Jun 2015: Please e-mail, fax, or telephone your request to Hampton Research. Fax and e-mail Technical Support are available 24 hours a day. ... 24. 0.2 M Calcium chloride dihydrate, 0.1 M Sodium acetate trihydrate pH 4.6, 10% v/v 2-Propanol. -
Sup Fig 1
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/YopJSup.pdf11 Nov 2008: 24 h after transfection cells were serum-starved for 16 h and then stimulated with 20 ng/ml TNF-α. ... 24. 26. 28. 30. 32. 34. 36. 38. 40. 42. inte. -
Journal of Visualized Experiments www.jove.com Copyright © 2018…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/JOVE2018.pdf19 Apr 2018: JBS6 JenaBioScience CS-106L 24 Incomplete factorial based on AmS (pH 4.6-8.5). ... JBS7 JenaBioScience CS-107L 24 Incomplete factorial based on MPD (pH 4.6-8.5). -
Structure of a cholinergic cell membraneNigel Unwina,1 Edited by ...
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnasS22.pdf4 Nov 2022: The broken yellow lines identify the well-documented ribbon of δ subunit-linked receptor dimers(14, 15, 18, 24), one of which is labeled in each leaflet to indicate the subunit ... Biophys. J. 92, 3960–3967 (2007). 24. H. W. Chang, E. Bock, Molecular -
MD1-118 Hippocrates additive screen
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/LMB%20plates/Addit-03.pdf13 Oct 2020: D3 21 1.5 % Caffeic acid †D4 22 1.5 % D-()-Quinic acidD5 23 1.5 % Gallic acid monohydrate†D6 24 1.5 % N-vanillylnonanamide (capsaicin synthetic)†E1 25 1.5 % -
PNAS202120006_proof
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/pnas%20Lee%202022.pdf18 Dec 2022: Edited by James Berger, Johns Hopkins Medical Institute, Baltimore, MD; received November 2, 2021; accepted February 24, 2022. ... However, the molecular basis for this extraordinary motoractivity remains enigmatic, despite a significant number of -
NPGRJ_NSMB_1158 1..8
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nsmbFtsK2006.pdf12 Oct 2006: coli chromosome segregation byorienting the FtsK translocase. EMBO J. 24, 3770–3780 (2005). ... J. Mol. Biol. 343, 547–557 (2004). 24. Ohnishi, T., Hishida, T., Harada, Y., Iwasaki, H. & -
Cryo‐EM structure of the full‐length Lon protease from Thermus…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Lon%20Coscia%202021%20FEBS.pdf14 Nov 2021: 24]. All subsequent image-processing steps were performed. using single-particle reconstruction methods in RELION. ... improved cryo-electron microscopy. Nat Methods 14,. 331–332.24 Zhang K (2016) Gctf: Real-time CTF determination. -
pone.0107211 1..10
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/leoa2014.pdf3 Sep 2014: replacing the leoA̧ leoB and leoAB genes with a chloramphenicolresistance cassette as described [24]. ... 24. Datsenko KA, Wanner BL (2000) One-step inactivation of chromosomal genes. -
The structure of human thyroglobulin
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/TG_Nature2020.pdf31 Jan 2020: Replacing all acceptor. tyrosines (Y) with phenylalanines (F) prevents hormone formation (4 acceptor residues: 24, 2573, 2766 and 1310). ... Eur. J. Endocrinol. 138, 227–231 (1998). 24. Dunn, J. T., Kim, P. -
61 Ultramicroscopy 25 (1988) 279-292 North-HoUand, Amsterdam 279…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/ultram88.pdf1 Apr 2016: to be a pure phase object [21-23]; combination of several images of the same specimen recorded over a range of un-derfocus values [20,24]. ... 24] R.H, Vogel. S.W. Provencher, C.-H. von Bonsdorff, M. Adrian and J. -
30 Apr 2004 18:9 AR AR214-BB33-09.tex AR214-BB33-09.sgm…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/annrev2004.pdf1 May 2004: 2004.Structural/functional homology betweenthe bacterial and eukaryotic cytoskele-tons.Curr. Opin. Cell Biol.16:24–31. 3. ... Micro-biol. 24:905–15. 32. Erickson HP. 1995. FtsZ, a prokaryotichomolog of tubulin?Cell 80:367–70. -
Acetylation of MEK2 and I{kappa}B kinase (IKK) activation loop…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/YopJ.pdf28 Nov 2006: CELL. BIO. LOG. Y. radation of -catenin (24). It is thus possible that YopJ-relatedAvrA may also possess an acetyl transferase activity allowing itto inactivate IKK thereby exerting its effect on ... Gastrointest Liver Physiol 287:G220 –227.24. -
INVITED REVIEW Mechanosensory molecules and circuits in C. elegans ...
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/2015-2.pdf10 Dec 2014: These mayinclude proposed extracellular tethers such as MEC-1 orMEC-9 [24] or alternatively may involve other, as yet uniden-tified sensor proteins. ... Nature 349(6310):588–593. doi:10.1038/349588a0. 24. Du H, Gu G, William CM, Chalfie M (1996) -
mmi_4133.fm
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Spo0J%202004.pdf30 Jun 2004: Saturation of the DNA occurs at an approximate equimolar concentration of 24 bp binding sites to dimeric Spo0J (full length). ... 1C). It is possible to gelfiltrate a stable complex of full-length Spo0J with 16–24 bpoligonucleotides of. -
DOI: 10.1126/science.1136985 , 120 (2007); 316Science et al.Maria C.…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Alonso_Science.pdf6 Apr 2007: 151, 1081 (2000).24. R. B. Case, S. Rice, C. L. Hart, B. -
MD1-116-117 Morpheus III-Morpheus III HT-96
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/LMB%20plates/LMB22.pdf13 Oct 2020: 3 8.5 30 % Precipitant Mix 21-23 B11 1.5 % Vitamins mix 0.1 M Buffer System 3 8.5 30 % Precipitant Mix 31-24 B12 1.5 % Vitamins mix ... 8.5 30 % Precipitant Mix 32-24 F12 0.6 % Antibiotics mix 0.1 M Buffer System 3 8.5 37.5 % Precipitant Mix 42-25 G1 1.2
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