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7 Jul 1999: Joint CCP4 and ESF-EACBM Newsletter for Protein Crystallography, 33, 22-24. Gabius, H. ... Biophys.Biomol. Struct. 24, 551-577. Sambrook, J., Fritsch, E. F. & Maniatis, T.

20 Apr 2002: Crystal Structure of a Calcium-Phospholipid Binding Domainfrom Cytosolic Phospholipase A2. (Received for publication, September 24, 1997). ... 1991) J. Appl. Crystallogr. 24, 946 –95072. Nicholls, A. (1992) GRASP: Graphical Representation and Analysis

11 Aug 2020: 23.4 4.1, 43.6 6.9 µm2 respectively) or Eed/ (24.3 4.5,42.2 4.7 µm2, respectively) ESCs are significantly larger thanequivalent chromosomes isolated from WT ... 24 %. 70 %. async m-arrested. coun. t. PI intensity. Cardiomyocytes.

15 Mar 2018: For correspondence:. egarner@g.harvard.edu. †These authors contributed. equally to this work. Competing interests: The. authors declare that no. competing interests exist. Funding: See page 32. Received: 03 October 2017. Accepted: 21 February

30 Dec 2002: ParM subunits are assem-bled head to tail into a protolament. Each successive subunit is rotatedby 166 and translated by 24.5 AÊ to create a helical lament. ... J. Appl. Crystallogr., 24,946950. Laskowski,R.A., MacArthur,M.W., Moss,D.S. and Thornton,J.M.

21 Nov 2012: Fig. 2A). As a positive controlfor cytokinesis failure, depletion of hIST1 resulted in a 14-foldaccumulation of multinucleated cells (24, 27). ... Mol Biol Cell 21(19):3293–3303. 24. Agromayor M, et al. (2009) Essential role of hIST1 in cytokinesis.

11 Nov 1999: response to oxidative stress. Mol. Carcin. 24, 1524 (1999). 14. Yin, Y. ... UK, 1992). 24. CCP4. Collaborative Computing Project 4: A suite of programs for protein crystallography.

29 Sep 2017: 4OA: 4q0a. 4OA (CSD). 24. 4OA: 4q0a. 4OA (CSD). 25. 4OA: 4q0a.

20 Sep 2004: Molecular Cell, Vol. 15, 951–964, September 24, 2004, Copyright 2004 by Cell Press.

11 Jun 1997: Based on sequence similarity and structural studies,modular PH, EF-hand and C2 domains have beendescribed in many proteins.22-24 PLCδ1 PH domainshares a high degree of structural similarity with ... 24. Kawasaki H, Kretsinger R (1994) Calcium-binding

30 Jul 2002: age but lost its platform subdomain. The high degree of observation that there is a pool of epsin located at theGolgi [24]. ... 1996). Methods used in271, 12111–12116.the structure determination of bovine mitochondrial F1 ATPase.24.

15 Dec 2008: at a pixel size of 0.24 to 0.42 nm. Electron cryomicroscopy imaging was performed at 300 kV either on an. ... 23. Figure S4 FPlasmid-free control. 24. A B. C D. Figure S5 low-copy number R1 plasmid (pKG491).

16 Oct 2008: 24),and an imidazoquinoline (5) inhibits the serine-threonine kinasePDK1 (ref. 25). The structural basis for these interactions isnot known. ... e) Cell cycle analysis by flow cytometry following treatment withPP121 or PI-103 (2.5 mM) or with vehicle (0.1

24 Sep 2004: Received for publication, January 5, 2004, and in revised form, March 22, 2004Published, JBC Papers in Press, March 24, 2004, DOI 10.1074/jbc.M400023200. ... 2002) Biochim. Biophys. Acta 1579, 219 –224. 24. Bishop, N., Horman, A., and Woodman, P.

14 Dec 2010: Egg-laying. 17. Neurophysiology in C. elegans. 24. Electrophysiology. 25. vi. Calcium Imaging. ... receptors. The worms will eventually (in 12 – 24 hrs) adapt to high levels of.

18 Oct 2008: C-terminal 24 residues downstream from the putative. internalization motif in the DAT C-terminus to Tac. ... Of interest, a recent study has suggested a lipid-binding. capacity also for the PDZ domain (24).

27 Apr 2015: ProcNatl Acad Sci USA 80(16):4922–4925. 24. Howard J, Hudspeth AJ, Vale RD (1989) Movement of microtubules by single kinesinmolecules. ... J Bacteriol 196(24):4304–4314. 30. Westermann S, et al. (2006) The Dam1 kinetochore ring complex moves

12 Oct 2012: Cocrystals were obtained with Bt TubR and a 24-bpdouble-stranded oligonucleotide (tubC24). ... Each point isplotted over the 12th bp of the 24-bp hairpin, with the sequence shown be-low.

24 Oct 2018: Slide 19. Slide 20. Slide 21. Slide 22. Slide 23. Slide 24.

24 Oct 2017: Tutorial: Ligand Fitting with Coot. CSHL 2014. October 24, 2017. 1 Introduction.

22 May 2008: sona. l use. onl. y. ANRV343-BB37-04 ARI 24 April 2008 15:9. ... l use. onl. y. ANRV343-BB37-04 ARI 24 April 2008 15:9. Endocyticevents.

20 Apr 2002: PLC Activity in Mixed Micelles—The assay of hydrolysis of PIP2 andPI was based on methods described previously (19, 21, 24, 25). ... Thecalcium dependence of PIP2, PIP, and PI hydrolysis was analyzed usinga range of calcium buffers as described

30 Apr 2010: S1) (24). Doc2b/ mice did not expressthe remaining exons and lacked Doc2b immuno-reactivity. ... Neurosci. 24, 1226 (2004).16. A. Maximov, O. H. Shin, X. Liu, T.

24 Sep 2015: at MRC Lab of M. olecular Biology on September 24, 2015. ... evaluate apoptosis, using the method described previously (24).TIRF Imaging—HSG cells were seeded and cultured over-.

4 Oct 2007: Diffraction data NATI, 30.7–2.8 Å, all dataR-factor, R-freea 0.24 (0.38), 0.28 (0.45)B-factorsb 54.9 Å2, 1.74 Å2. ... EMBO J 24: 270–282. Lim GE, Derman AI, Pogliano J (2005) Bacterial DNA segregation bydynamic SopA polymers.

20 Apr 2002: Deletion variants of PLC-N1 and PLC-Qlacking this second lobe are inactive, probably dueto destabilising the fold of the enzyme [23,24].Although sequence similarity throughout the EF-. ... Bio-phys. Res. Commun. 211 (1995) 364369. [24] Y. Emori, Y. Homma,

4 Aug 2008: filament. The EMBO Journal (2008) 27, 2230–2238. doi:10.1038/. emboj.2008.152; Published online 24 July 2008.

3 May 2017: this state (21, 23, 24). ... One of the conse-quences of such a switch, namely, permitting cooperative assembly of a single-strandedfilament, have been discussed previously (24, 27, 29, 39); however, such a switchconfers additional surprising

21 Feb 2011: Frequent observations of a complex network ofconnected tubules with an extensive surface area implythat considerable EHD2-driven fusion occurs betweentubulated liposomes [24]. ... 2009) The BAR domain superfamily: membrane-molding macromolecules. Cell 137

29 May 2017: 24/40. Refinement Stabilizers. Using Coot's Real Space Refinement – (in default mode). – ... Slide 20. Slide 21. Slide 22. Slide 23. Slide 24. Slide 25.

9 Sep 1998: 10.2 (24.1) 9.0 (17.9)Riso (%). c 32.5Phasing power 1.29 (0.77)Anomalous phasing power 1.33 (0.57)Resolution in outer shell (Å) ... 2.20 3.24–3.0Rfactor 0.190Rfree (10% of data) 0.258.

1 Dec 2010: Smc1DDSmc3AAA. Smc1Smc3AAA. Kd = 22 nM Kd = 29 nM Kd = 24 nM. ... The Kd for Smc1DD and Smc3AAA hingedomain association is 29 nM (middle panel) and 24 nM for Smc1 wild-type and Smc3AAA (right panel).

8 Jun 2006: Long. sample columns were used. Scans (averaging 10 readings) were taken at 280 nm at 24 hr.

17 Mar 2003: Cell, Vol. 104, 71–81, January 12, 2001, Copyright 2001 by Cell Press. Complexins Regulate a Late Step in Ca21-DependentNeurotransmitter Release. cellular Ca21 concentration. Usually, only a fraction oftethered vesicles in any given synapse is

19 Apr 2002: Molecular Cell, Vol. 9, 773–788, April, 2002, Copyright  2002 by Cell Press. Molecular Architecture of SMC Proteinsand the Yeast Cohesin Complex. other eukaryotes so far studied and several have alsobeen implicated in sister chromatid cohesion

17 Jun 2005: 223 40 pA; 5M GDP-β-s and 0.5 mM ATP, 83 9 fF and 173 24 pA. ... 2004). Monitoring clathrin-mediated endocytosis during synaptic activity. WmJ. Neurosci. 24, 2004–2012.nNeves, G., and Lagnado, L.

24 Jan 2009: Mean F values (SD)for wild-type (3.9 24.9) and Syt-1RK (2.8 21.6) did not differ, whereas those for Syt-14W (15.1 16.8) differed ... vesicles. During a 5-min incubation in de-polarizing medium, an average of 22.6 3.5 vesicles inwild-type cells and 24.6

19 Jun 2006: Keywords: prions; retrovirus budding; exosome; endosomal transport;lysosome; multivesicular bodySubmitted 24 May 2006; accepted 31 May 2006.

15 Nov 2011: B) Expression of the PLCδ1PH–GFP construct in Sf9Op1D cells inhibitedcell fusion that was triggered 24 h post-transfection. ... Rev. Mol. Cell Biol. 5, 133–147. 24 Mettlen, M., Pucadyil, T., Ramachandran, R.

4 Oct 2010: Distances 20Å were investigated using four-pulse DEER (24–27), whereasshorter distances were investigated using continuous wave EPR(28, 29) (see “Experimental Procedures”).The DEER measurements are illustrated with the example ... Mutant

2 Feb 2004: Membrane transport between compartments in eukary-otic cells requires proteins that allow the budding andSCISSION of nascent cargo vesicles from one compartmentand their targeting and fusion with another. Just asSNARES are proposed to be essential

7 Apr 2010: 18–20]), the BAR domain con-taining proteins [14,21–24], EHD2 [25], the complexes of clathrinwith adaptor and accessory proteins (see for reviews e.g.

20 Apr 2002: 24). Thetopology of the domain is identical to that of phospholipase Cd1and the recently reported calcium-independent C2 domainfrom protein kinase Cd (23), whereas it is a circular permuta-tion of ... 24. Sutton, R. B., and Sprang, S. R. (1998) Structure

24 Sep 2015: Cancer Res; 72(14) July 15, 2012 Cancer Research3644. on September 24, 2015. ... Cancer Res; 72(14) July 15, 2012 Cancer Research3648. on September 24, 2015.

22 Jul 2007: Fisher et al, 2001).Received: 24 January 2007; accepted: 9 May 2007; published online:14 June 2007. ... methane) and set in 24-well Cryschem plates (Hampton Research),with 400 ml of reservoir solution.

22 Mar 2005: A–E) HeLa cells were transfected with myc-epsinR for 24 hr and stained for the indicated markers. ... Cell 50,(Roche). Immunofluorescence experiments were carried out 24 hr 453–463.after transfection, as described previously (Johannes et al., 1997).

25 Nov 2012: JBiol Chem 283:31227–31236. 24. Burke JE, et al. (2008) Interaction of group IA phospholipase A2 with metal ions andphospholipid vesicles probed with deuterium exchange mass spectrometry.

20 Apr 2002: baculovirus expression of Src family kinases were madeby subcloning the cDNAs (24) encoding activated Src (Y527F Src),activated Fyn (Y531F Fyn), and activated Lck (Y525F Lck) into thebaculovirus transfer vector ... Some of the additional sites couldbe

20 Apr 2002: Negatively charged regions are shown in red and positively chargedin blue (ranging from 18 kT/e to 24 kT/e). ... Crystallogr.heavy-atom parameter refinement for multiple isomorphous re- 24, 946–950.placement and multiwavelength anomalous diffraction

16 Aug 2008: Molecular Cell. Article. Molecular Mechanism of Sequence-DirectedDNA Loading and Translocation by FtsKJan Löwe,1, Antti Ellonen,2 Mark D. Allen,3 Claire Atkinson,2 David J. Sherratt,2 and Ian Grainge2,1MRC Laboratory of Molecular Biology, Hills