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LMB Screen Scoring Sheet
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/LMB%20plates/LMB_21.pdf23 Sep 2016: B9 18% v/v ethanol, 0.1M bis tris propane pH 7.1. B10 24% v/v ethanol, 0.04M magnesium chloride hexahydrate, 0.1M HEPES pH 7.8. ... D7 15% w/v PEG 3350, 0.1M MES pH 6.2. D8 24% w/v PEG 3350, 0.15M sodium chloride, 0.05M HEPES pH 6.8, 2% v/v -
doi:10.1128/mBio.00800-13. 4(6): .mBio. Midcell DNA Translocase FtsK…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/mbio2013bisicchia.pdf23 Dec 2013: coli FtsKN used in the in vivo experiments, 24 amino acids forthe E. ... Mol. Microbiol.27:257–268. 24. Steiner W, Liu G, Donachie WD, Kuempel P. -
Dynamic Filaments of the Bacterial Cytoskeleton
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/annrev2006.pdf19 Apr 2006: ANRV277-BI75-21 ARI 24 February 2006 2:50. RE V. I E W. ... sona. l use. onl. y. ANRV277-BI75-21 ARI 24 February 2006 2:50. -
Sensory Neurons Arouse C. elegans Locomotion via Both Glutamate and…
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/2015-4.pdf30 Jun 2015: For this experiment, we utilized transgenicanimals that express rat TRPV1 capsaicin receptors in ASH neurons [24]. ... In these animals,capsaicin treatment evokes ASH-mediated avoidance behaviors [24]. A 5-hour capsaicin treat-ment had little effect on L4 -
se060101051p
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/epsin/EM/ford.pdf3 Dec 2004: The COOH-termi-nal domains of these proteins contain clath-rin-binding motifs (3, 24 ), as well as Asp-Pro-Phe (DPF)-like a- and b-adaptin–. ... De Camilli, A. T.Brunger, J. Cell Biol. 149, 537 (2000). 24. -
TBS may template.qxd
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/epsin/EM/mcmahon.pdf3 May 2002: In each of thesecases, purified soluble factors and syntheticliposomes are sufficient to reconstitute coatformation [21–24]. ... Proc. Natl. Acad. Sci. U. S. A. 95,11199–11204. 24 Matsuoka, K. -
Structure of the hexagonal surface layer on Caulobacter crescentus…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/s-layer-nmicrobiol201759.pdf6 Apr 2017: After optimization, the native RsaA crystals weregrown at 19 C by sitting-drop vapour diffusion in a drop composed of 100 nl ofreservoir solution (0.07 M KSCN, 24% (wt/vol) ... Trypsin cleavage and LPS staining. Full-length RsaA (2 mg ml–1) was -
Bacterial actin MreB assembles in complex with cell shape protein RodZ
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embo2010RodZMreB.pdf17 Feb 2010: The crystals were cryoprotected with mother liquorcontaining 24.8% PEG400 and flash frozen in liquid nitrogen. ... The plate was incubated at 301Cand imaged after 24 h. DrodZ complementation assays and microscopy. -
doi:10.1016/j.jbbm.2005.12.008
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/rfcloning2006%20published.pdf19 Apr 2006: Thereverse primer annealed to the vector with 24 bases complementary to the 3V end of the point ofinsertion. -
PII: 0896-6273(95)90037-3
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/neuron95.pdf4 May 2004: Number of Receptors Phase Residual" Weight in Final. scFv35 Film number 4280 3560 23.5 0.18 Film number 4282 4360 24.2 0.23 Film number 4295 4820 16.1 ... Biochemistry 24, 1212- 1221. -
bic054a
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/LundmarkBJ2008.pdf11 Aug 2008: with negative curvature, is unaffected [24]. ... Biol. Chem. 264, 21066–21072. 24 Antonny, B., Bigay, J., Casella, J. -
53091 361..366
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/McMahon01020.pdf3 Dec 2004: 24). Thus our experiments point to a pioneering role forepsin in vesicle budding as it provides both a driving force and a linkbetween membrane invagination, clathrin polymerization and AP2complex recruitment. ... beta 2-adaptin appendage domain. EMBO J. -
BAR Domains as Sensors ofMembrane Curvature: TheAmphiphysin BAR…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/PeterBARdomains.pdf30 Jan 2004: recruit clathrin to membranes. Mammalian am-phiphysin2 has several clathrin-binding sites (24–26), and we found that the wild-type, but notmut1, efficiently recruited clathrin to liposomes(fig. ... It is interesting to note that thestructure of the -
se060101047p
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Itoh%20et%20al.pdf23 Nov 2004: Quantitativerepresentations of co-sedimentation assay data of GST-ENTH domains from (D) epsin and (E)AP180 (24) with 0.5% of the indicated phospholipids in the liposomes. ... X-ray Crystallography and NMR (Yale Univ. Press,New Haven, CT, 1993). 24. -
2623.tif
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jcb90.pdf23 Jan 2012: glycine receptors (for recent reviews, see references 5, 7, 24, 33). -
early in DNA damage response and regulateRAP80 localization. Many ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/MartensSytPap07.pdf16 Oct 2009: Wang, P. R. Andreassen, A. D. D’Andrea, Mol. Cell.Biol. 24, 5850 (2004). ... S4). Because of its Ca2+-independentinteraction with the tSNAREs and/or PtdIns(4,5)P2in the plasma membrane (13, 24), syt1 in the syn-aptic vesicle is closely associated with -
Supplementary Figure 1Lysophosphatidic acid acyl transferase (LPAAT)…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndoSupFigs.pdf4 Jan 2006: 35. 24. 21. Bead. s pos. t clea. vage. Purif. ied cl. -
Prokaryotic cytoskeletons: protein filaments organizing small cells
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nrmicro.2017.153.pdf18 Jan 2018: Nature Reviews Microbiology, (2018). doi:10.1038/nrmicro.2017.153 -
www.landesbioscience.com Autophagy 805 Autophagy 6:6, 805-807; August …
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/20574157.pdf2 Aug 2010: Submitted: 05/24/10. Revised: 06/03/10. Accepted: 06/03/10. Previously published online: www.landesbioscience.com/journals/autophagy/article/12577Correspondence to: Roger L. -
The patterned assembly and stepwise Vps4-mediated disassembly of…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Hurtig%202023%20sciadv.ade5224.pdf16 Jul 2023: 1B and fig. S1A), as previously re-ported (24), and to an increase in the percentage of cells containingelevated levelsof theESCRT-IIIproteins,CdvB,CdvB1, andCdvB2(fig. ... Coverslipswere placed in 24-well plates and spun down with labeled cell -
FigS1
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/SchmidBetaPaper/BetaHubSup.pdf7 Sep 2006: AP180(27), Eps15(6). Dynamin1,2,3 (89,30,24), Amph1(24). Eps15R(52), AP180(5), Dynamin1(1). Amph1(15), Eps15R(2). Amph2(40), Epsin1(1). -
Features critical for membrane binding revealed by DivIVA crystal…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/emboj201099a.pdf20 May 2010: 21.7) 24.1 (ano. 14.1) 3.8 4.3 (ano. 2.5) 2.8. Phasing Se SAD Ta SAD MR I SAD MRNumber of sites 6 5 8Mean FOM -
Jason William Chin Page 1 of 18 MRC Laboratory ...
https://www2.mrc-lmb.cam.ac.uk/ccsb/wp-content/uploads/2024/01/Chin-cv-26012024.pdf29 Jan 2024: R. James. Nature Struct. Mol. Biol. 2017. 24: 1155-1163. (109) Expanding and Reprogamming the Genetic Code. ... W. Chin. Biochem. Soc. Trans. 2006. 34: 328-329. (24) A Genetically Encoded, Fluorescent Amino Acid. -
FtsK in motion reveals its mechanism for double-stranded DNA…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/FtsK_Jean_2020_PNAS.pdf8 Jun 2020: This arrangementis reminiscent of the single spiral staircases observed in ring-shaped helicases (24). ... orienting the FtsK translocase. EMBO J. 24, 3770–3780 (2005).10. S. Bigot, O. -
Biallelic Variants in ASNA1, Encoding a Cytosolic Targeting Factor of …
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/CircGen_2019.pdf1 Dec 2019: due to impaired ASNA1-mediated nuclear targeting.24 Though emerin staining showed apparently normal localization of the protein in our patients, it did reveal the characteristic abnormal nuclear morphology previously described ... J Cardiovasc Dev Dis. -
The ER membrane protein complex promotes biogenesis of sterol-related …
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/JCS_2019.pdf18 Jan 2019: At 24 h post transfection, cellswere treated with MG132 (5 µg/ml) or DMSO (vehicle control) for 8 h andanalysed by flow cytometry. -
mc4604.qxd
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/current%20opinion%20micro%202001.pdf12 Nov 2001: Mol Microbiol 1996, 22:231-237. 19. Margolin W: Themes and variations in prokaryotic cell division.FEMS Microbiol Rev 2000, 24:531-548. ... J Cell Biol 2000, 151:F19-F21.See annotation to [24•]. 24. Kiessling J, Kruse S, Rensing SA, Harter K, Decker EL, -
The EMBO Journal Vol.18 No.9 pp.2364–2371, 1999 Tubulin-like…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/EMBO%20paper%201999%20FtsZ.pdf20 Apr 1999: All four calculationsproduced the same filament arrangement. After two cycles of unbendingand correction for the contrast transfer function, amplitudes and phasesfrom 24 images were merged. ... FtsZ sheet structure. and schematic plots of protein -
2168 | K. von der Malsburg et al. Molecular ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/93_vonderMalsburg-_K_MBC_2015.pdf11 Aug 2015: C. A 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25Fract. -
CURRICULUM VITAE
https://www2.mrc-lmb.cam.ac.uk/groups/bienz/wp-content/uploads/sites/23/BienzCV_March2024.pdf11 Apr 2024: eucaryotes. Adv Genet 24, 31-72. 17. Saari G & Bienz M (1987) The structure of the Ultrabithorax promoter. ... Drosophila embryos. EMBO J 8, 2677-2685. 24. Tremml G & Bienz M (1989b) An essential role of even-skipped for homeotic gene. -
doi:10.1016/j.molcel.2006.06.019
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Ftsk%20mc%202006.pdf12 Aug 2006: with a FEI F20 microscope at 62,0003 magnification with 24 to 25. -
Technician Commitment Evaluating Impact through Self-Assessment & …
https://www2.mrc-lmb.cam.ac.uk/wordpress/?wpdmdl=35486&ind=168994162466726 Jul 2023: open day to be held on 1st July 2023. Please provide a 24-month action plan, detailing future plans to ensure your organisations addresses the themes of the Technician. -
740 Research Paper Calcium triggers calcineurin-dependent synaptic…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Marks_McMahon1998.pdf21 Mar 2003: Correspondence: Harvey T. McMahonE-mail: hmm@mrc-lmb.cam.ac.uk. Received: 3 March 1998Revised: 24 April 1998Accepted: 5 May 1998. ... synapse. Cell 1994, 79:717-727. 24. DeBello WM, Betz H, Augustine GJ: Synaptotagmin andneurotransmitter release. -
Organization of spines on the dendritesof Purkinje cellsJohn O’Brien…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnas06.pdf25 Jul 2006: 2004) Nat. Rev. 5, 24 –34.4. Matus, A. (2000) Science 290, 754 –758.5. ... T., Wong, R. O. L. & Lichtman, J. W. (2000) Neuron 27, 219 –225.24. -
ANALYSIS Integrating molecular and network biologyto decode…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Schmid_Nature2007.pdf22 Aug 2007: 2a)7,8,23,24. 1MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK. ... Nature Biotechnol. 24, 1270–1278(2006). 42. Smith, C. J., Grigorieff, N. & Pearse, B. -
Insight 1 Dec McMahon NS
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/NatureReview2005.pdf4 Jan 2006: EMBO J. 24, 1537–1545 (2005). 25. Cremona, O. et al. Essential role of phosphoinositide metabolism in synaptic vesiclerecycling. ... Structure of the Sec23/24-Sar1 pre-budding complexof the COPII vesicle coat. Nature 419, 271–277 (2002). -
doi:10.1016/j.jmb.2004.12.031
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jmb05.pdf24 Jan 2005: doi:10.1016/j.jmb.2004.12.031 J. Mol. Biol. (xxxx) xx, 1–23. ARTICLE IN PRESS. Refined Structure of the Nicotinic AcetylcholineReceptor at 4 Å Resolution. Nigel Unwin. MRC Laboratory of MolecularBiology, Hills Road, CambridgeCB2 2QH, UK. -
Crystal Structures of Bacillus subtilis Lon Protease
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/londumanjmb2010.pdf20 Jul 2010: electron microscopy data show that negativelystained images of E. coli Lon particles appearhexameric,24 although surprisingly, electron cryo-microscopy images of S. -
The ER membrane protein complex is a transmembrane domain insertase
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017c.pdf27 Jan 2018: 24, 2533–2542 (2005).10. B. M. Abell et al., J. Biol. Chem. ... essentially as described in detail previously (24, 36–38) and summarized in brief below. -
The current approach to initial crystallization screening of proteins …
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Gorrec_undersamplid_2013.pdf14 May 2013: Appl. Cryst. 24, 409–411.Kimber, M. S., Vallee, F., Houston, S., Necakov, A., Skarina, T., Evdokimova,. -
15591370691369 1..30
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/elife_2019.pdf29 May 2019: doxycycline, then analyzed 24 hr later by fluorescent microscopy. Doxycycline withdrawal for 24 hr limited the amount of reporter mRNA in cells to. ... Figure 5D and Figure 5—figure supplement 1C). Only a 24 kD band matching the size of TMED. -
TTC5 mediates autoregulation of tubulin via mRNA degradation
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2019.pdf19 Nov 2019: another TTC5 function (22–24), because the effects were not rescued by two unrelated point mutants of TTC5 that perturb autoregulation by different mechanisms. ... Mol. Cell 8, 71–84 (2001). doi:10.1016/S1097-2765(01)00277-5 Medline. 24. X. Hu, R. -
Bb9e55.qxd
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Vallis_McMahon1999.pdf21 Mar 2003: J Biol Chem 1996, 271:27979-27982. 24. Liu JP, Yajima Y, Li H, Ackland S, Akita Y, Stewart J, et al.: Molecularinteractions between dynamin and G-protein beta gamma-subunits ... Cells were plated 24 h prior to trans-fection (day 0) at a density of 1.4 -
IntroductionThe endosomal system functions as an internal…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/SNX2Cullen2005.pdf17 Jan 2006: treatment. Results were normalised againstthe amount of CD8 reaching the TGN after 24 minutes in control-treated cells, and were expressed the s.e.m. ... 9A).Compared with control cells, suppression of SNX1 led to a54.57.8% reduction in the amount of anti -
Articleshttps://doi.org/10.1038/s41594-019-0196-z 1MRC Laboratory of…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Burmann%202019%20NSMB.pdf4 Mar 2019: Cell 163, 1628–1640 (2015). 24. Bürmann, F. et al. Tuned SMC arms drive chromosomal loading of prokaryotic condensin. ... Curr. Biol. 21, 12–24 (2011). 27. Badrinarayanan, A., Reyes-Lamothe, R., Uphoff, S., Leake, M. -
Biol. Chem., Vol. 381, pp. 993 – 999, September/October ...
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/BiolChem%20Helical%20Tubes.pdf13 Sep 2000: Cell Struct. Funct. 24,285 – 290. Lu, C.L., Stricker, J., and Erickson, H.P. ... EMBO J. 16, 5455 –5463. Received May 24, 2000; accepted July 14, 2000. -
KS_2012_A5_Meeting.indd
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/conferences/2012_A5_Save_the_Date.pdf19 Sep 2011: Late-Breaking Abstract:October 24, 2011. Early Registration:November 21, 2011. Deadlines. late-breaking research • cross-disciplinary collaboration • networking opportunities • peer-reviewed meeting programs. -
x16412u141
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/MaoAP180-2001.pdf17 Mar 2003: 3 246,217 67,191 99.5 (100) 24.0 (4.4) 6.4 (27.1)SeMet l3 (0.9712) 2.3 248,253 67,292 99.7 (100) 22.9 ... Neuron 24, 143–154. Schmid, S.L. (1997). Clathrin-coated vesicle formation and proteinsorting: an integrated process. -
validation-tutorial.dvi
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/web/tutorial/validation-tutorial.pdf24 Oct 2017: Tutorial: Validation with Coot. CSHL 2017. October 24, 2017. 1 Preamble. -
No Job Name
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/vandenent2008ftsq.pdf4 Mar 2008: FtsQ is a bitopic membraneprotein of 31 kDa (Robinson et al., 1984), consisting of ashort cytoplasmic tail (residues 1–24), a transmembraneregion (25–49) and a periplasmic domain of approximately226 residues
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