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News - Cambs 24
https://www2.mrc-lmb.cam.ac.uk/archive/articles/Road_named_after_legendary_scientist.pdf1 Feb 2013: Copyright 2013 Archant Regional Ltd. All rights reserved. News - Cambs 24 http://www.cambs24.co.uk/news/cambridge_road_to_be_na. -
Structure and superorganization of acetylcholinereceptor–rapsyn…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnas13.pdf3 Jul 2013: 20%of the receptors showed restricted diffusion in small domains of50 nm, whereas the majority of the receptors were immobile (24).Confocal microscopy of overexpressed rapsyn in nonmuscle. ... The cross-linking of receptors by rapsyn,therefore, seems to -
MR_LMB
https://www2.mrc-lmb.cam.ac.uk/download/molecular-replacement/?wpdmdl=18799&refresh=669a8523c6fc9172140265911 Jun 2013: 24 (12) RF/TF. 3 (1) Pack. 3 (1) Refined. 307 (283) RF/TF. ... 68 (64) Pack. 24 (2) Refined. 32 (20) RF/TF. 22 (17) Pack. -
doi:10.1128/mBio.00800-13. 4(6): .mBio. Midcell DNA Translocase FtsK…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/mbio2013bisicchia.pdf23 Dec 2013: coli FtsKN used in the in vivo experiments, 24 amino acids forthe E. ... Mol. Microbiol.27:257–268. 24. Steiner W, Liu G, Donachie WD, Kuempel P. -
pnas201313978 4601..4610
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/pnas2013duman.pdf23 Dec 2013: AnezrA-null mutant forms slightly longer cells (20%) comparedwith wild-type cells (23), but an ftsA null mutant has problemsdividing and forms long filamentous cells (24). -
The current approach to initial crystallization screening of proteins …
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Gorrec_undersamplid_2013.pdf14 May 2013: Appl. Cryst. 24, 409–411.Kimber, M. S., Vallee, F., Houston, S., Necakov, A., Skarina, T., Evdokimova,. -
Qrb1300006 1..40
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/qrb13.pdf30 Jul 2013: Transition to open-channel form 23. 5.1. Introduction 23. 5.2. Spray-freeze-trapping experiments 24. -
Cooperative Recruitment of Dynamin and BIN/Amphiphysin/Rvs (BAR)…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/JBC_Meinecke2013.pdf19 Mar 2013: nexin 9 (SNX9) (23, 24). It has a BAR and an SH3 domain thatbinds to dynamin.The involvement of BAR domain proteins in dynamin activ-. ... J. Biol. Chem. 278, 46772–46781. 24. Soulet, F., Yarar, D., Leonard, M., and Schmid, S. -
Deubiquitinases Sharpen Substrate Discrimination during Membrane…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/82_Zhang_ZR_Cell_2013.pdf5 Aug 2013: panel) plasmids were cotransfected with a fixed. amount (100 ng) of CD4 and analyzed 24 hr later by. -
, 130081, published 7 August 20133 2013 Open Biol. ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Open%20Biol.-2013-TakedaCombined.pdf25 Sep 2013: compacted by the ingressingcleavage furrow (figure 2e, wild-type, 16 – 24 min; electronicsupplementary material, movie S3). ... GFP alone, the cleavage furrowstarted to ingress within 4 min after anaphase onset at2.24 mm min – 1 (s.e.m. -
MR_LMB
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_crystallography_course_2013/lectures/MR_LMB.pdf11 Jun 2013: 24 (12) RF/TF. 3 (1) Pack. 3 (1) Refined. 307 (283) RF/TF. ... 68 (64) Pack. 24 (2) Refined. 32 (20) RF/TF. 22 (17) Pack. -
Supplement_Final
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/81_Shao_S_Mol_Cell_2013.pdf16 May 2013: Molecular Cell. Article. Listerin-Dependent NascentProtein Ubiquitination Relieson Ribosome Subunit DissociationSichen Shao,1,2 Karina von der Malsburg,1,2 and Ramanujan S. Hegde1,1MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge -
Expression, purifica0on, characteriza0on, and crystalliza0on So how…
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_crystallography_course_2013/lectures/20130430_Cryst_course.pdf2 May 2013: Acta Crystallogr. D Biol. Crystallogr. 60, 203–204 (2004). Solubility Screen. Protein characteriza0on 1-‐10mg/ml 100nl/drop Incubate 1-‐24 hrs. ... Or Jena Bioscience JBScreen Solubility HTS. Solubility Screen. Protein characteriza0on 1-‐10mg/ml -
pone.0052401 1..15
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Podosome2012/journal.pone.0052401.pdf10 Jan 2013: clear amphipathic characteristics and fold on membrane binding. [4]. A 24 and 28 amino acid long helix, H0, would be possible for. ... Members of the PAK family are major. effectors of the Rho GTPases CDC42 and Rac (reviewed in [24]). -
Kevin Cowtan, cowtan@ysbl.york.ac.uk 2012 Automated phase…
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_crystallography_course_2013/lectures/densitymod12-ppt.pdf28 May 2013: Slide 19. Slide 20. Slide 21. Slide 22. Slide 23. Slide 24. -
Slide 1
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_crystallography_course_2013/lectures/processing.pdf13 May 2013: approach. 24/44. 24/44. Integration by profile fitting. Based on the assumption that spots corresponding to fully recorded reflections in the same region of the detector (and on images nearby -
Expression, purifica0on, characteriza0on, and crystalliza0on So how…
https://www2.mrc-lmb.cam.ac.uk/download/03-protein-expression-crystallization-and-mutagenesis-meindert-lamers/?wpdmdl=18793&refresh=669a852400fdd17214026602 May 2013: Acta Crystallogr. D Biol. Crystallogr. 60, 203–204 (2004). Solubility Screen. Protein characteriza0on 1-‐10mg/ml 100nl/drop Incubate 1-‐24 hrs. ... Or Jena Bioscience JBScreen Solubility HTS. Solubility Screen. Protein characteriza0on 1-‐10mg/ml -
Twinning and other pathologies Andrey Lebedev CCP4 OD-‐structures…
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_crystallography_course_2013/lectures/pathologies_and_twinning.pdf11 Jun 2013: 04/06/2013 Crystallography Course LMB 24. (Pseudo)merohedral twining is impossible. 432 622. ... Data processing ( -‐1 ). Molecular replacement ( P1 ). Refinement ( P1 ). R / R-‐free = 0.24 / 0.31. PDB: 1yup ( P1 ). PDB: 1yup. Zanuda. ( P21 ) -
Twinning and other pathologies Andrey Lebedev CCP4 OD-‐structures…
https://www2.mrc-lmb.cam.ac.uk/download/twinningaandaotherapathologiesa-andreyalebedeva-a/?wpdmdl=18788&refresh=669a8524e6ea9172140266011 Jun 2013: 04/06/2013 Crystallography Course LMB 24. (Pseudo)merohedral twining is impossible. 432 622. ... Data processing ( -‐1 ). Molecular replacement ( P1 ). Refinement ( P1 ). R / R-‐free = 0.24 / 0.31. PDB: 1yup ( P1 ). PDB: 1yup. Zanuda. ( P21 ) -
Kevin Cowtan, cowtan@ysbl.york.ac.uk 2012 Automated phase…
https://www2.mrc-lmb.cam.ac.uk/download/automated-phase-improvement-and-model-building-kevin-cowtan-cowtanysbl-york-ac-uk/?wpdmdl=18786&refresh=669a8525207bf172140266128 May 2013: Slide 19. Slide 20. Slide 21. Slide 22. Slide 23. Slide 24.
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