Search

25 Jun 2007:

25 Jun 2007: As a negative control, inject pure chloroform without any lipid (this will test for lipid dependence of any structures seen, such as whether clathrin baskets form in solution instead of clathrin

25 Jun 2007: We then collaborated with Peter Cullen (Bristol University) to test the in vivo function of sorting-nexin1 in membrane trafficking. ... The model stands the test of time but we would still like a direct measurement of the force produced by GTP hydrolysis.

6 Apr 2007: Fur-ther work will be required to test this prelim-inary interpretation. Existing data indicates that ATP binding hasprofound effects on the kinesin head, recon-figuring the active site and shifting

2 Jun 2007: protein saturation on liposomes. To test more directly whether the dimer seen in the crys-. ... tutive helix. To test for a role for helix 1 in membrane bind-.

4 Oct 2007: tion. Mutant parR genes were tested for functionality in their. natural genetic context on test plasmids derived from low-. ... As shown in Figure 4A, test plasmids. containing the ParR171 K6E, R7S and K8E mutations severely.

22 Aug 2007: We would suggest that a good test for proteins that function ashubs only when clustered in space and time is that overexpression ofsuch a protein should not have any phenotype on

22 Aug 2007: sufficient overexpression to test if the network can be distorted and overexpressed.

8 Dec 2007: To test whether MIMs have a role in sorting, we mutated motifs inboth Vps2 and Did2.

15 May 2007: To directly test the. role of each residue of the IVF motif, in the context of. ... Methods. Errors bars were obtained by calculating the standard error and relevance of the difference assessed with a Student t-test.

22 Jul 2007: In addition,. using mouse models as a preclinical test, as we have done in.

23 Jul 2007: To further test this notion, we performed two independent. measurements. First, we monitored by SPR analysis the.

14 Jun 2007: Structural insight into the ESCRT-I/-II link and itsrole in MVB trafficking. David J Gill1,, Hsiangling Teo1, Ji Sun2,Olga Perisic1, Dmitry B Veprintsev3,Scott D Emr2 and Roger L Williams1. 1MRC Laboratory of Molecular Biology, Medical Research

15 Aug 2007: Molecular Biology of the CellVol. 18, 3681–3691, September 2007. The Role of p58IPK in Protecting the Stressed EndoplasmicReticulumD. D. Thomas Rutkowski,† Sang-Wook Kang,†‡ Alan G. Goodman,Jennifer L. Garrison, Jack Taunton, Michael G. Katze

28 Sep 2007: To test if a polybasic stretch close to the tip of the helical domainwas involved in lipid binding, we monitored lipid binding of K327D,K328D and also F322A (Fig.

11 May 2007: UNIT 17.9Rational Design and Evaluation of FRETExperiments to Measure ProteinProximities in Cells. Defining the repertoire of interactions thata particular protein can undergo is crucialfor understanding its function and regulation.Characterizing