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3 Dec 2004: Liposome tubulation, sedimentation and electron microscopy. Synthetic liposomes contained 10% cholesterol, 40% phosphatidylethanolamine, 40% phosphatidylcholine, and 10% of the test lipid (in most cases phosphatidylinositol(4,5)bisphosphate).

20 Sep 2004: However, the disorder makes it difficult todesign specific mutants to test this hypothesis.its C terminus (Figure 1D). ... To test whether the S525N mutation Is There Any Turnover of Cohesin Subunits.

3 Dec 2004: test-ed by sedimentation assays using liposomesor lipid tubules (19, 27, 28). ... Each assay contained0.05 mg/ml protein. (B) Liposomes containing 10%cholesterol, 40% phosphatidylethanolamine, 40%phosphatidylcholine, and 10% of a test lipid wereprepared

3 Dec 2004: test-ed by sedimentation assays using liposomesor lipid tubules (19, 27, 28). ... Each assay contained0.05 mg/ml protein. (B) Liposomes containing 10%cholesterol, 40% phosphatidylethanolamine, 40%phosphatidylcholine, and 10% of a test lipid wereprepared

30 Jan 2004: T-tubule net-work (7 ). To test if this tubulating ability is dueto the Drosophila amphiphysin BAR domain,we mutated pairs of basic residues to gluta-mates, Lys161 Lys163 on the ... S3B). As a functional test of the amphiphysin BARdomain, we tested the

3 Dec 2004: Liposome tubulation, sedimentation and electron microscopySynthetic liposomes contained 10% cholesterol, 40% phosphatidylethanolamine,40% phosphatidylcholine, and 10% of the test lipid (in most casesphosphatidylinositol(4,5)bisphosphate).

30 Jan 2004: T-tubule net-work (7 ). To test if this tubulating ability is dueto the Drosophila amphiphysin BAR domain,we mutated pairs of basic residues to gluta-mates, Lys161 Lys163 on the ... S3B). As a functional test of the amphiphysin BARdomain, we tested the

3 Dec 2004: test-ed by sedimentation assays using liposomesor lipid tubules (19, 27, 28). ... Each assay contained0.05 mg/ml protein. (B) Liposomes containing 10%cholesterol, 40% phosphatidylethanolamine, 40%phosphatidylcholine, and 10% of a test lipid wereprepared

30 Jun 2004: parS. sequence5. ¢. -TGTTCCACGTGAAACA-3. ¢. (Fig. 1A and B). To test forthe ability of wild-type and truncated proteins to carry outDNA binding and spreading, we used an agarose gel

23 Nov 2004: MBP asa test substrate in a 50-ml reaction. ... Statistical comparisonswere made with the two-way analysis of variance(ANOVA) and two-tailed t test with Bonferroni cor-rection; P , 0.05 indicated statistical significance.

29 Dec 2004: In order to test whether stimulated [3H]bumetanide binding would be re- tained in membranes after activation of transport by agents other than catecholamines, we examined specific binding in membranes from

2 Feb 2004: 4) have been used asassembly tests for other members of the dynamin family(see Oligomerization web site in Online links). ... This clathrin-centricmodel for endocytosis does not, however, take intoaccount the role of dynamins in

1 May 2004: At least theparC/ParR/M system is simple enoughto test this possibility in vitro.

1 Nov 2004: An analogous salt link is formed betweenattractive proposal. To test this, we made an N-terminalArg83 of Vps25 and Asp548 of Vps36.

8 Nov 2004: To test this possibility, we analyzed the behavior of PrP. constructs containing mutations in the transmembrane do-.

3 Dec 2004: Liposome tubulation, sedimentation and electron microscopySynthetic liposomes contained 10% cholesterol, 40% phosphatidylethanolamine,40% phosphatidylcholine, and 10% of the test lipid (in most casesphosphatidylinositol(4,5)bisphosphate).

6 Oct 2004: Rap and PtdIns(3,4,5)P3 Activate ARAP3 Rho GAPActivity In VivoTo test whether a Rap family member activates ARAP3’sRho GAP activity in vivo, we performed more

23 Nov 2004: Refinement statisticsR factor (%) 0.177RFree (%) 0.224RFree test set size (%) 0.051<B> Å2 (mean, overall) 27.98Nreflections (Nfree) 27464 (1467)Natoms (Nwater) 2467 (297)Rmsd bond length (Å) 0.03Rmsd

19 Nov 2004: The fact that the domain swap is possible and a report describing two-stage unfolding kinetics of FtsZ24 prompted us to test whether the N- and C-terminal domains of FtsZ ... To test for bound nucleotide, FtsZ was precipitated using 0.5 N cold HClO4, and

15 Apr 2004: Each bar represents the mean SD (n 10), with statistically significant differences (P 0.01 using t test) being observed between the two conditions. ... multiple days.aTreatment is significant (P 0.01) compared with untreated cells using t test.

6 Dec 2004: Data Analysis—Data are given as means S.E. Comparisons be-tween means were performed using one-tailed Student’s t test forpaired data with GraphPad PrismTM software.

21 Dec 2004: Differences among eachconstruct were statistically significant (p 0.01 by Student’s t test).(B) Ten nanograms per well of constructs encoding the indicatedsignal sequences fused to TF were analyzed for