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Bacterial actin MreB assembles in complex with cell shape protein RodZ
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embo2010RodZMreB.pdf17 Feb 2010: Before theexperiments, proteins were dialysed to 20 mM Tris–HCl, pH 7.5,1 mM EDTA, 1 mM sodium azide, 200 mM NaCl. ... Tris–HCl, pH 9.0, 1 mMEDTA, 1 mM NaN3, 200 mM NaCl, 2 mM TCEP and prespun at138 000 g for 10 min, 41C. -
Chapter 20 In Vitro Dissection of Protein Translocationinto the ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/62_Sharma_AS_MMB_2010.pdf30 Apr 2010: 4. 100 mM CaCl2: Dissolve in water. Keep at 4C.5. 200 mM EGTA. ... A good range to start is between 30and 200 µL of packed bead volume. -
A positively charged channel within the Smc1/Smc3 hinge required for…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embosmcjingekurze2010.pdf1 Dec 2010: Smc1DDSmc3AAA. ARS207 ARS231. 150 160 170 180 190 200. 10.0. 5.01.00.0. ... 5.0. 1.00.0. Smc1DD E1158QSmc3AAA. ARS207 ARS231. 150 160 170 180 190 200. -
Doc2b Is a High-Affinity Ca2+ Sensor forSpontaneous Neurotransmitter…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/GroffenScience2010.pdf30 Apr 2010: ST-D. oc2b. C2AB. 4AGS. T-Do. c2b. C2AB. K23. 7,319. E. 200 400 600 800 1000 12000. ... Neurosci. 25,. 5127 (2005).29. V. Chan-Palay, Z. Anat. Entwicklungsgesch. 134, 200. -
12 January 2010 CAMBRIDGE UNIVERSITY REPORTER 13 Section B: ...
https://www.reporter.admin.cam.ac.uk/reporter/2009-10/special/09/section_b.pdf12 Jan 2010: 5 – – 200.5 Press – – 35.2 – – – 35.2 Unallocated re Contributory Pension Scheme – – – – (183.0) – (183.0). ... Total 1,880.8 200.5 35.2 247.7 (183.0) – 2,181.2. -
University of Cambridge Research Horizons Issue 11
https://www.cam.ac.uk/system/files/issue_11_research_horizons.pdf5 Jan 2010: And since we have another 6 metres to go before we hit bedrock, thedeepest archaeological trench in northAfrica has potentially a 200,000-year-oldstory to tell.’. ... at high Cross on madingley Road,the turner centrifuge at the Department -
University of Cambridge Research Horizons Issue 13
https://www.cam.ac.uk/system/files/issue_13_research_horizons.pdf10 Nov 2010: Breastfeeding accounts for up to a third ofall mother-to-child transmissions of hiV,approximately 200,000 babies every year,primarily in sub-Saharan Africa. -
Features critical for membrane binding revealed by DivIVA crystal…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/emboj201099a.pdf20 May 2010: The EMBO Journal, (2010). doi:10.1038/emboj.2010.99 -
MRC plate poster 18 8 2010 hr
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/PresentationsOther/Poster%20Plates.pdf6 Sep 2010: reservoir holds 200 µl. use robot or manually dispense 0.1 - 10 µl of reservoir and protein. -
The Acetyltransferase Activity of the Bacterial Toxin YopJ ofYersinia …
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Mittal_JBC2010.pdf4 Oct 2010: Bound material was elutedfrom the resin using 10 ml of 2 N hydrochloric acid, lyophilizedto remove the acid, and taken up in 200 l water. ... time in a 200-l volume using a FluoroMax-2spectrofluorometer (Horiba Yvon Spex).
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