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19 Apr 2006: Miroux personal communication), 200 AM dNTP’s (Roche), 1!reaction buffer,2.5 units PfuTurbok (Stratagene) in a total volume of 50 Al. ... TCF1 200 3–6 2.0 4.6 4/4 OK Yes nd. TCF2 200 3–6 2.0 4.6 4/4 OK Yes nd.

31 May 2006: Consultancy income 1,500 2,250 3,000 3,750 4,500Consultancy fees (1,200) (1,800) (2,400) (3,000) (3,600)Health/software – net of Dept share 18 16 ... 15 15 15Interest 25 25 25 25 25Department charge (195) (293) (390) (488) (585)Admin charge by CE (140)

24 Jan 2006: warehouse sites, S1, S2 and S3 are given in Table 4. X coordinate Y coordinate OutputF1 300 180 200F2 400 400 70F3 200 360 240F4 100 270 160.

21 Nov 2006: 587 = 1,200,793. ... 29,200 Balance at beginning of year. 188,913Balance at end of year.

26 Jan 2006: A lossof 100–200 l is usually observed. Purification of Dynamin and Its Mutants from E. ... Scale bars are 200 nm in. length. [52] IN VITRO RECONSTITUTION OF ENDOCYTIC STAGES 607.

16 Jan 2006: 266 200 9,601 9,450Experimental Psychology 1,650 47 1,940 97 15 66 2,702 1,063 50 3,815 3,862Genetics 912 67 4,066 19 13 102 ... 116Counselling Service – – – – 343 27 506 (136) – 370 506Combination Room – 3 – 48 (9) 1 47 (4) – 43 40Dental

12 Oct 2006: c) Superdex 200 gel filtration of E. coli FtsKg in the presence of DNA. ... Storage buffer was. 20 mM Tris-HCl, 200 mM NaCl, 1 mM EDTA and 1 mM sodium azide.

16 Jan 2006: 666 (5,032,210) – – 32,945,493Developing World Fund 3,390,273 144,524 55,679 200,203 – (279,211) 533,972 3,845,237Cambridge University Press Donation 7,444,324 ... 316,040 (210) 315,829 – (316,040) 1,200,433 8,644,547.

4 Jan 2006: fter. th. rom. bin cl. eava. ge o. f GST. ). 0.01. 0.1. 1. 10. 0 100 200 300 400. ln OD600. ... 1. 10. 0 100 200 300 400. ln OD600. pPLSC (E.coli LPAAT).

4 Jan 2006: fatty acid-free BSA and 0.01% Triton X-100 with substrate concentrations (20–200 mM LPA) at 25 8C and 37 8C with 14C-labelled oleoyl-CoA (Amersham) at aspecific ... J. Cell Biol. 155, 193–-200 (2001). 11. Huttner, W. B. & Schmidt, A.

16 Jan 2006: 42,999 – 2,282 59,454 59,44311,585 14,670 – 14,670 (18,200) (3,530) – 1,867 9,922 9,907. – ... 220 (53,220) (0) – – (0) –. 73,919 104,885 – 104,885 (6,412) 98,472 – 11,916 184,308 184,27961,055 15,141 – 15,141 (23,200) (8,059) –

8 Jun 2006: 150. 200. 247. Supplementary Figure 2. Sequence alignments and 3D models of C.

28 Feb 2006: Totals 5,432 3,816 1,426 190 1,370 17. WomenMaintained 3,098 2,200 737 161 742 7Independent 1,646 1,078 446 122 504 8Other and Overseas 466

28 Nov 2006: Acetyl Transferase Assays. GST-MEK2 (0.5 g) (purified by usingstandard protocols for GST-tagged protein purification) wasincubated with varying amounts (0 –200 ng) of (His)6-YopJ-wt ... Stimulation of MEK and Erk phosphorylation by various

12 Nov 2006: Briefly, a 1 ml sample of each PX domain was loaded onto a Superdex 200 column (Amersham-Pharmacia) equilibrated and eluted with 20 mM Tris-HCl buffer, pH 7.4, containing

17 Jan 2006: Bars, 200 nm. 4535Sorting Nexin-2 and CI-MPR retrieval. mammalian orthologue of the yeast retromer componentVps17p (Haft et al., 2000). ... J. Cell Biol. 155, 193-200. Gagescu, R., Gruenberg, J. and Smythe, E.

12 Aug 2006: agent (200 ml in 700 ml water) was added and Pi release monitored. ... tios of these proteins were mixed (in buffer A plus 200 mM NaCl;.

21 Nov 2006: 587 = 1,200,793. ... 29,200 Balance at beginning of year. 188,913Balance at end of year.

8 Jun 2006: 20. –10. 0. 10. 20. 30. 200 210 220 230 240 250Wavelength (nm). ... Binding to 50 nm liposomes (p:pellet; s: supernatant) and tubulation of 200 or 400 nm liposomes(monitored by electron microscopy) are shown.

28 Sep 2006: 50 nm. 100 nm. 200 nm. I. Supplementary Figure 2. The crystal structure of BDLP in GDP-associated and nucleotide-free conformation.