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19 Apr 2006: Miroux personal communication), 200 AM dNTP’s (Roche), 1!reaction buffer,2.5 units PfuTurbok (Stratagene) in a total volume of 50 Al. ... TCF1 200 3–6 2.0 4.6 4/4 OK Yes nd. TCF2 200 3–6 2.0 4.6 4/4 OK Yes nd.

26 Jan 2006: A lossof 100–200 l is usually observed. Purification of Dynamin and Its Mutants from E. ... Scale bars are 200 nm in. length. [52] IN VITRO RECONSTITUTION OF ENDOCYTIC STAGES 607.

12 Oct 2006: c) Superdex 200 gel filtration of E. coli FtsKg in the presence of DNA. ... Storage buffer was. 20 mM Tris-HCl, 200 mM NaCl, 1 mM EDTA and 1 mM sodium azide.

4 Jan 2006: fter. th. rom. bin cl. eava. ge o. f GST. ). 0.01. 0.1. 1. 10. 0 100 200 300 400. ln OD600. ... 1. 10. 0 100 200 300 400. ln OD600. pPLSC (E.coli LPAAT).

31 May 2006: Consultancy income 1,500 2,250 3,000 3,750 4,500Consultancy fees (1,200) (1,800) (2,400) (3,000) (3,600)Health/software – net of Dept share 18 16 ... 15 15 15Interest 25 25 25 25 25Department charge (195) (293) (390) (488) (585)Admin charge by CE (140)

4 Jan 2006: fatty acid-free BSA and 0.01% Triton X-100 with substrate concentrations (20–200 mM LPA) at 25 8C and 37 8C with 14C-labelled oleoyl-CoA (Amersham) at aspecific ... J. Cell Biol. 155, 193–-200 (2001). 11. Huttner, W. B. & Schmidt, A.

8 Jun 2006: 150. 200. 247. Supplementary Figure 2. Sequence alignments and 3D models of C.

28 Nov 2006: Acetyl Transferase Assays. GST-MEK2 (0.5 g) (purified by usingstandard protocols for GST-tagged protein purification) wasincubated with varying amounts (0 –200 ng) of (His)6-YopJ-wt ... Stimulation of MEK and Erk phosphorylation by various

12 Nov 2006: Briefly, a 1 ml sample of each PX domain was loaded onto a Superdex 200 column (Amersham-Pharmacia) equilibrated and eluted with 20 mM Tris-HCl buffer, pH 7.4, containing

17 Jan 2006: Bars, 200 nm. 4535Sorting Nexin-2 and CI-MPR retrieval. mammalian orthologue of the yeast retromer componentVps17p (Haft et al., 2000). ... J. Cell Biol. 155, 193-200. Gagescu, R., Gruenberg, J. and Smythe, E.