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1 Nov 2004: on Superdex 200 16/60 equilibrated in buffer C. ... A 200–250 g aliquot of GST-tagged pro-taining 3.1% PEG 35000, 0.1 M Tris acetate (pH 8.5), 1.36 M sodiumtein and 600–700 g of

24 Sep 2004: The high-spin. Acknowledgmentssupernatant was loaded in batch onto Glutathione Sepharose4B.The resin was washed with buffer B (50 mM Tris [pH 7.5] and 200.

6 Oct 2004: indicated dishes were treated with 200 nM wortmannin 40 minRap on ARAP3 Rho GAP activity (Figure 3C).

4 May 2004: 510 100 1;0 200 2;0 310 3;0 400 4 ; 0 500.

16 Aug 2004: Tris (pH 7.5),200 mM NaCl and 5 mM MgCl2 and eluted separately.Following calibration, 10 ml of ZapA protein at 10 mg/ml was injected and eluted as a ... 1 mM EDTA,1 mM NaN3 and 200 mM NaCl was placed in thesample compartment of an Epon double-sector

30 Jun 2004: Spo0J] (mM)0 25 50 100 200 300 25 50 100 200 300. ... The protein was buffer exchanged into20 mM Tris, pH 8.5, 200 mM NaCl using a Sephadex G25 gelfiltration column (Amersham).

19 Nov 2004: sZ-N. TmFt. sZ-C. TmFt. sZ-N. C. TmFtsZ TmFtsZ-N. TmFtsZ-C TmFtsZ-NC. Wavelength (nm)200 210 220 230 240 250 260. ... 081% (w/v) malachite green and 2 ml of water were mixed and 200 µl was added to 50-µl reactions contain-ing proteins or inorganic

15 Apr 2004: Calcu-lations employed torsional dynamics at 50 000 K for 1000steps of 15 ps, then a first slow cooling stage of torsionaldynamics (200 cycles each of 1000 steps of 15

17 Mar 2004: J. Cell Biol. 155,193-200.

23 Nov 2004: Cells were incubated A series of frames depicting a GFP-SNX1-decorated tubule trans-with either 200 ng/ml TxR-EGF (A) or 20 g/ml Alexa568-transferrin (B) porting ... The scale barrepresents 200 nm. pressed at high levels, membrane association of wild-