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21 Mar 2003: Dynamin GTPase assay and sedimentation assayThese assays have been described previously [20,27] and furtherdetails are given in the Supplementary material. ... Multimerised dynamin was then sedimented by ultracentrifuga-tion at 80,000 g for 20 min.

16 Jul 2003: and POPC/POPE/phos-phatidylinositol 3,4,5-trisphosphate (77:20:3) vesicles. ... a Increase in Kd relative to binding POPC/POPE/PtdIns(4,5)P2 (77:20:3) vesicles.b Not measurable.

20 Nov 2003: Crystals were equilibrated in 20% glycerol for cooling to 100K. The crystal asymmetric unit contains one molecule, and the crystals belong to spacegroup P3121, cell dimensions a = b = 49.6Å, c = ... 1mM TCEP. Sedimentation was at 11,000 rev/min, 20.0C,

21 Mar 2003: whereas glutamate release was essentially unaffected (96 9% ofmaximal; n = 3) in the presence of 20 µM cyclosporin A. ... Fol-lowing two washes, the synaptosomes were spun briefly a third timeand resuspended in lysis buffer (20 mM Hepes).

13 Jun 2003: 20) and Trusca et al. (21), using a MBP-SulAfusion or a protein A-SulA fusion, respectively, found SulA doesinhibit the GTPase activity of FtsZ. ... The reservoir was 7.5%polyethylene glycol (PEG) 20,0007.5% PEG monomethyl ether55080 mM sodium formate0.1

21 Mar 2003: COS cells. (a) (c). (b) (d)Extract incubated with GST–α-adaptin. over 20 cells quantitated (Figure 4a–c) and the blockadewas rescued by coexpression of dynamin. ... Although we have not demonstrated a lack of dynaminrecruitment to coated pits per se,

9 May 2003: least two chemosensory neurons (AWA and AWC) has been measured to -20 mV, which. ... membranes. After 15-20 minutes of equilibration the cells were transferred into fresh.

17 Mar 2003: vesicle endocytosis. J. Neurosci. 20, 8667–8676. Ye, W., and Lafer, E.M. ... I. Developmental expres-sion and cellular localization of the F1-20 protein and mRNA.

16 Jan 2003: The Journal of Cell Biology The Rockefeller University Press • 0021-9525Volume 160 January 20, 2003http://www.jcb.org/cgi/doi/10.1083/jcb.200208023.

15 Sep 2003: Thus with asingle image, there was too much noise for few, ifany, of the layer-lines to be distinguished convin-cingly beyond a resolution of about 20 AÊ. ... Common to all views are the 20 AÊwide vestibules on either side of the membrane,and a more

17 Mar 2003: presence of 20 ml 50% slurry of glutathione-following induction with 0.2 mM IPTG. ... Cell Biol. 20, 313–332.pathway in viral infection. EMBO J. 17, 4585–4593.

4 Apr 2003: The signal retention improved by only 10% at low resolution (20 A), but by progressively greater amounts at higher resolutions, up to - 40% at 9 8. ... 20]; variable twist has been corrected by ap- plying an innovative real-space algorithm in a study of

17 Mar 2003: controls, as well as release phase while the corresponding value in controlCPX I/II double mutant (2/2, 2/2; B) synapses (20,000-fold magnifi-. ... initial synaptic release probability is reduced in these concentrations of 0.5–20 mM, while the Mg21

5 Sep 2003: to-back interacting PB1 domains, with a freebuffer C (20 mM Tris, pH 7.4, 100 mM NaCl, and 5 mM DTT). ... C.D., Anderson, K.E., Butler, P.J., Lavenir, I., Perisic, O., Hawkins,GST/Par6b binding assays) and run on 20% Phast SDS gels.

27 Nov 2003: EMBO J 2001,20:5813-5821. 49. Hinshaw JE: Dynamin and its role in membrane fission.Annu Rev Cell Dev Biol 2000, 16:483-519. ... EMBO J2001, 20:1819-1828. Structural/functional homology between the bacterial and eukaryotic cytoskeletons Amos, van den Ent

6 Feb 2003: factor inthe same proteoliposomes was only. 15–20% of the control,raising the possibility that its transport may require stimula-tory factor(s) not needed by Prl. ... Only after the synthesis of an additional 20 resi-dues did the PrP–Prl intermediate

27 Oct 2003: being represented by 3 of the 20 ESTs for human golgin-(C) The oligomerization state of the His6-GRIP domain derived by 245 in the current GenBank database. ... The complex was concentrated ton X-100 in PBS, blocked with 20% (v/v) fetal calf serum/0.25%.

19 Jun 2003: holes, using a 300-kV field emission microscopeincorporating a top-entry liquid-helium-cooled stage10 (magnification, £36,800; defocusrange, 9,000–16,000 Å; dose, ,20 electrons Å22). ... 3H]diazofluorene. J. Biol. Chem. 273, 8659–8668 (1998). 20.

9 Jan 2003: 20, 2003 213–222http://www.jcb.org/cgi/doi/10.1083/jcb.200208023. ... All GST fu-sion proteins were purified from bacterial extracts by incubation with glu-tathione-Sepharose beads, followed by extensive washing with 20 mMHepes, pH 7.4, 150 mM NaCl,

16 Jan 2003: The Journal of Cell Biology The Rockefeller University Press • 0021-9525Volume 160 January 20, 2003http://www.jcb.org/cgi/doi/10.1083/jcb.200208023.

11 Sep 2003: Usually there are four or more stretches of about 20 hydrophobic amino acids per subunit or domain: the correct length for an a-helix to span the hydrophobic lipid chains. ... the contacting helices are inclined to one another by even greater angles:

24 Sep 2003: MR Z. EMMERICHIntroduction to political philosophy. Tu. 12. (Eight lectures, beginning 20 Jan.). ... PROF. T. MANSTEADEmotions. W. 12, Th. 10 (Four lectures, 13, 19, 20, 26.

12 Aug 2003: Criminological Research 5 7 12 — — —M.Phil. Criminology 8 12 20 — — —M.Phil. ... Archaeology and Anthropology 10 16 26 — — —Archaeology 20 30 50 18 15 33Biological Anthropology 12 26 38 5 4 9Social Anthropology 20 27 47 20 20 40.

23 Jul 2003: pen. sion. s0. 20. 40. 50. 20. 20. 40. 10. 20. ... 40. 10. 20. 40. 20. 20. 40. 20. 20. 419. Mis.

24 Sep 2003: 18, M. 20, W. 22DR D. J. TOLHURSTThe Motor System (Five lectures) M. ... GIBSONTestes Tu. 9 (20 Jan.); W. 4 (21 Jan.) (Two. Lectures)DR J.

10 Jan 2003: L. FOWDENSexual differentiation, M.10 (20 Jan.) (One Lecture) L.T. DR J. ... 12 (20 Feb.); Tu. 12 (25 Feb.) (Four Lectures) L.T.DR A.

6 May 2003: 20 CAMBRIDGE UNIVERSITY REPORTER [SPECIAL NO. 14. Consolidated Cashflow Statement for the year ended 30 September 2002. ... At 1 October 2001 20.47 3.74 0.20 2.75 27.16. TANGIBLE FIXED ASSETSUCLES.

30 Jun 2003: EMBO J. 20, 5383 – 5391. 12 Ma, J. and Lindquist, S.

23 Sep 2003: 9 (90 min). DR S. CASTELVECCHINineteenth-century music II (Twelve lectures,. beginning 15, 20 Jan.) Tu. ... 9.30 (90min). (Eight lectures and seminars, beginning 20 Jan.)Tu.11 (90 min).

24 Sep 2003: ELLARMacromolecules in the Cell. (Five lectures, beginning. 20 Oct.)DR M. TESTERMembranes: Molecular Superstructures. ... Eight lectures, 20 Apr.–6 May.)Please note the early start of this course.

28 Jan 2003: 1,700,649 — — (223,329) 1,218,822 1,218,822. 20 CAMBRIDGE UNIVERSITY REPORTER [SPECIAL NO. ... 476 — 21,476 (20,000) 1,476 — (23,377) 120,716 120,706. —

23 Jan 2003: PWF Club locationsChemical Engineering 16 10 220 72Economics 7 13 85 252Law (to December 2001) 5 5 20 66. ... Research Councils 61 (0.6%) 46 (0.5%) 37 (0.8%) 30 (0.7%)Other external (world-wide) 674 (6.3%) 489 (4.9%) 20 (0.4%) 24 (0.5%).

9 Feb 2003: for everyt 0, 1, 2,C. Assume that the initial number of wolves is 20 and the initialnumber of rabbits is 11, so thatx0 20, 11 I.a.

23 Sep 2003: MCKITTERICK. Medieval Research Seminar. Th. 5–7Weekly seminars beginning 20 May,History Faculty Boardroom. ... M.5 Fortnightly seminars beginning 20 October, DiracRoom, Fisher Building, St John’s College.

21 Mar 2003: COS cells. (a) (c). (b) (d)Extract incubated with GST–α-adaptin. over 20 cells quantitated (Figure 4a–c) and the blockadewas rescued by coexpression of dynamin. ... Although we have not demonstrated a lack of dynaminrecruitment to coated pits per se,

28 Nov 2003: 20,000Sum transferred to General Reserve XXV from Estates £7,686,464 at 16%. ... 20,886Maintenance. 19,709 Transfers from other accounts: £Purchase of books, binding, printing, etc.

28 Nov 2003: 20,000Sum transferred to General Reserve XXV from Estates £7,686,464 at 16%. ... 20,886Maintenance. 19,709 Transfers from other accounts: £Purchase of books, binding, printing, etc.

28 Nov 2003: 20,000Sum transferred to General Reserve XXV from Estates £7,686,464 at 16%. ... 20,886Maintenance. 19,709 Transfers from other accounts: £Purchase of books, binding, printing, etc.

28 Nov 2003: 20,000Sum transferred to General Reserve XXV from Estates £7,686,464 at 16%. ... 20,886Maintenance. 19,709 Transfers from other accounts: £Purchase of books, binding, printing, etc.

16 Oct 2003: The. Jour. nal o. f Cel. l Bio. logy. JCB. Article. The Journal of Cell Biology, Volume 163, Number 2, October 27, 2003 257–269http://www.jcb.org/cgi/doi/10.1083/jcb.200306020 257. Formation of stacked ER cisternae by low affinity protein

24 Sep 2003: Accompanying texts may not be borrowed.). THE TEACHING ROOMS. 4 teaching rooms are available which have been designed to offer a flexible teaching and learning environment, catering for up to 20

24 Sep 2003: Fot those who spent 2002/2003 at MIT, and at10.20 for others.

24 Sep 2003: 19 Februaryweek 7 20 November week 7 26 Februaryweek 8 27 November week 8 4 March.

23 Sep 2003: Accompanying texts may not be borrowed.). THE TEACHING ROOMS. 4 teaching rooms are available which have been designed to offer a flexible teaching and learning environment, catering for up to 20

23 Sep 2003: WASHINGTONSeeley Library Introduction; 20 minute sessions as. follows:Tu. 7 Oct., 10, 10.30, 11, 2, 2.30W. ... Eight lectures and Eight classes]. W. 9, Tu. 2. European Fascism from 1919 (Paper 20)DR J.

23 Sep 2003: 9.30 a.m. – 4.30p.m. (full day workshop) 20 May(Thursday). STAFF DEVELOPMENT OFFICE.

23 Sep 2003: Tu. Th. 10, Tu.2 (Sixteen lectures and eight seminars,commencing 20 Jan.) Venue to bedecided.

21 Nov 2003: Officer's Number 2003

24 Sep 2003: GEUSS AND OTHERSM. 5 (Four seminars, fortnightly beginning 20 October). Dirac Room, Fisher Building, St.

28 Nov 2003: 20,000Sum transferred to General Reserve XXV from Estates £7,686,464 at 16%. ... 20,886Maintenance. 19,709 Transfers from other accounts: £Purchase of books, binding, printing, etc.