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19 Jan 2017: endogenous proteins with recombinant variantsrevealed the functional roles for all of the factorsand interactions. ... C) Immunoblots (IB) for triage components of control or BAG6 complex-depleted RRL replenished with recombinant BAG6 sub-complexes used

15 Nov 2011: To evaluatePtdIns(4,5)P2 content in the plasma membrane and to competefor PtdIns(4,5)P2 binding with endogenous PtdIns(4,5)P2-bindingproteins, we expressed PLCδ1PH (PH domain of ... Lowering the concentrations of PtdIns(4,5)P2available for interactions

14 Jun 2007: How do various ESCRT components link to other processes such as pH regulation of gene expression [51] or retrovirus budding [52]?

26 Aug 2021: Othersubunits of the 19S base were copurified atlower levels, consistent with partial assemblyof PSMC5 with endogenous base subunits pres-ent inthe lysate (table S5). ... Boosting HERC1 levelsusing CRISPR activation (31) of the endoge-nous HERC1 promoter

3 Sep 2014: coli ETEC H10407 was investigated. The protein at endogenous expression levels is easilydetected and this signal disappears in a leoA KO strain, demonstrating specificity.

27 Nov 2003: Immunofluorescence microscopy of endogenous Mbl in fixed cells gave. similar images.

16 Feb 2010: 2007). To maximize the severity of any phenotype, PrP+/ mice were used because endogenous PrP was observed in earlier studies to exacerbate CtmPrP-mediated neurodegeneration (Stewart et al., 2005).

16 Jul 2008: SDS-PAGE, andimmunoblotted using an antibody (3F4) that specifically detects transfected but not endogenous PrP. ... C) Biosynthesisand maturation of endogenous PrP in A4 and L-cells were assessedby pulse-chase analysis followed by immunoprecipitation

8 Nov 2004: Endogenous NF-kB (indicated by an aster-isk) serves as a loading control. ... Immunoblots were probed with antibodiesagainst PrP, GFP, and an endogenous protein, the beta subunit ofSec61.

24 Aug 2011: By Western blot analysis both endogenous and transfected Flot1 and GRAF1 were concentrated in Fraction 2.8 (Fig. ... 4 G, arrowheads). Endogenous dynamin labeled a significant population of struc-tures within Fraction 2.8 (Fig.

5 Feb 2019: and Scheer, F. Endogenous circadian system and circadian misalignment impact glucose tolerance via separate mechanisms in humans. ... The circadian clock is an endogenous biological mechanism that drives roughly 24h cycles in various physiological

17 Jan 2012: Instead, both endogenous and overexpressed GRAF1 were found enriched at small, round, vincu-lin-positive structures in a subset of cells (see Figure S1, C and D). ... Cells were acid-stripped and fixed and were then labeled for endogenous paxillin.

3 Dec 2004: endogenous AP2 (middle, AP6 antibody) and transferrin uptake (right). A strong.

14 Nov 2021: the ATP-dependent Lon protease- lessons from. endogenous protein and synthetic peptide substrates.

30 Apr 2010: Itdoes, however, stimulate the translation of some proteins(presumably because the endogenous levels of some tRNAsin the RRL are limiting), and it is possible that some RRLpreparations contain relatively low amounts ... 5. Endogenous RRL usually contains

18 Feb 2016: Ribosome profiling in the presence and absence of Dom34 has shown that an endogenous substrate for this pathway is Hac1, a cytosolically spliced mRNA whose incorrect ligation results in an mRNA ... The key open questions include the following: (i) What

23 Dec 2013: coli cells, we constructed C-terminal fusions of the fluorescentprotein YPet to wild-type FtsK and truncated derivatives of it ex-pressed from the endogenous wild-type chromosomal locus.

6 Oct 2004: The challenge now is to establish the molecularhave similar phenotypes: they retain a similar shape tolinks between endogenous ARAP3’s control of Rho andcontrol cells, but on PDGF stimulation their lamellipodiaArf

6 Dec 2014: Unfortunately, it is currently not possible to reliablyimage at endogenous levels of MinC with SIM.

11 Aug 2015: By contrast, endogenous SRP in a homologous mammalian system hasa remarkably subtle effect on translation elongation (Wolin and Walter, 1989). ... Here we demonstrate that endogenous SRP is efficiently recruited to mammalian ribosomescontaining a

9 May 2012: Hansen, New York: W.W. Norton, 135 – 155. Setterfield, M. (2006): Effective demand and endogenous money in a path-dependent economy: Towards a ›Moorian‹ credit supply curve – and a reconciliation between ... ed.), Complexity, Endogenous Money and

14 Dec 2010: amount of endogenous ACh at the synapse by preventing its metabolism by AChE. ... communication). 8. The endogenous agonist, serotonin, is a stable compound but was thought not.

4 Mar 2019: Articleshttps://doi.org/10.1038/s41594-019-0196-z. 1MRC Laboratory of Molecular Biology, Cambridge, UK. 2Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, UK. 3Bioanalytics, Institute of Biotechnology, Technische

18 Sep 2008: Ifn-PrP. Note the lack of changes to endogenous PrP in cells expressing. ... Thus, in both cases, Ifn-PrP is not being overexpressed relative. to endogenous PrP.

24 Jan 2009: It remains tobe determined whether endogenous Syt-1 regulates dense-core vesicle fusion pore expansion and if so, whether Syt-1utilizes membrane binding and curvature induction, or in-teractions with SNAREs, ... Syt Effector Interactions Regulate Fusion

6 Jun 2014: elifesciences.org. van den Ent et al. eLife 2014;3:e02634. DOI: 10.7554/eLife.02634 1 of 22. Bacterial actin MreB forms antiparallel double filamentsFusinita van den Ent1†, Thierry Izoré1†, Tanmay AM Bharat1, Christopher M Johnson2, Jan Löwe1.

31 Jan 2020: rTG appears to be indistinguishable by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and cryo-electron microscopy (cryo-EM) from endogenous TG (eTG) puri-fied from thyroid glands of patients

30 Jul 2013: inherent flexibility of the tubes and other factors, such as heterogeneity of the endogenous.

5 Aug 2013: Second, the system is typicallyorthogonal to endogenous pathways and utilizes a single definedligase. ... Furthermore, the absolute dependence on phospho-Vpu illustrates that endogenous ligases in this system do notperceive mCD4 as a misfolded protein

21 Feb 2011: Previous characterization ofBAR domain proteins has generally involved overproduc-tion, raising the need for in vivo studies characterizing theconsequences of BAR domain mutations in genes expressedat their endogenous level. ... these alterations affect

29 May 2019: For correspondence:. rhegde@mrc-lmb.cam.ac.uk. Competing interest: See. page 28. Funding: See page 28. Received: 11 March 2019. Accepted: 15 May 2019. Published: 16 May 2019. Reviewing editor: Maya. Schuldiner, Weizmann Institute,. Israel. Copyright

4 Mar 2013: considered as a determinant of savings. It is endogenous and passively adjusts to produce.

7 Mar 2012: 1. Sraffa’s ‘Given’ Quantities of Output and Keynes’s Principle of Effective Demand. Man-Seop Park. Korea University manseop@korea.ac.kr. [I]t is in the ‘present’ that the ‘normal’ rate of profits has always been firmly located. …

21 Nov 2014: Toaddress whether cohesin containing Smc3R61Qloads onto chromosomes, we compared the dis-tribution of green fluorescent protein (GFP)–tagged Smc3R61Q and wild-type Smc3 in livingcells carrying an untagged endogenous Smc3gene.

19 Nov 2019: Cite as: Z. Lin et al., Science 10.1126/science.aaz4352 (2019). REPORTS. First release: 14 November 2019 www.sciencemag.org (Page numbers not final at time of first release) 1. Alpha and beta tubulins form obligate heterodimers (hereaf-ter

7 Oct 2005: he same for both Crt and Prl-Crt and were comparableo the levels of endogenous Crt (Figure 5A). ... A) Crt/ cells stably expressing either Crt-GFP or Prl-Crt-GFPwere analyzed (in duplicate) by immunoblotting with anti-Crt andanti-Sec61β (an endogenous

30 Apr 1999: and endogenous rat brain vesicles. J. Biol. Chem. 268, 17240–17246 (1993).

18 Oct 2008: a high concentration of an endogenous PDZ-binding. Figure 5: The C-termini of GluR2 and of DAT fused to thesingle transmembrane protein Tac are sufficient for BARdomain-dependent coclustering with ... This. juxtanuclear clustering strongly resembles the

16 Jul 2023: 1A), hereafter calledVps4E209Q. This point mutation prevents AAA-ATPases like Vps4from hydrolyzing bound ATP and can therefore interfere with theactivity of endogenous Vps4 complexes when overexpressed (24,28).

11 Aug 2015: Because stable association of NEMF with 60S requires a peptidyl-tRNA (Shao et al., 2015), these complexes are likely to contain endogenous nascent chains. ... We examined this idea by analysis of endogenous RNCs on ER microsomes isolated from native

2 Aug 2010: 26]. The p101 subunitprotects cells from apoptosis when overexpressed, and thismay explain why it is a common site for insertion in T-celllymphomas induced by the MoFe2-MuLV retrovirus [27].The

7 Dec 2011: us needs endogenous and transformative solutions. We know that he or she.

25 Nov 2019: Nature Structural & Molecular Biology, doi:10.1038/s41594-019-0331-x

11 Nov 2011: This indicated that BirA has no detectable targetsendogenous to mammalian cells, and that the BioTag is notmodified by endogenous enzymes. ... Note that lowlevels of endogenous PrP are visualized at 35kDa in the SS-PrP samples probed with-PrP.

5 Jan 2016: ribosomes used for the cryo-tomography structure (16), thepresence or absence of heterogeneous endogenous nascentpolypeptides within Sec61 could not be determined conclusively.Thus, the conclusion that the Sec61 complex is constitutivelyopen

9 Sep 2015: Upon induction with tetracycline, both wild-type and mutant Sec61 transgenes (untagged) were expressed at similar levels as the endogenous protein (Figure 6—figure supplement 1C). ... Because we could not easily distinguish endogenous Sec61 from the

15 Sep 2011: To overcome problems caused by protein overexpression, our laboratory has used Zinc Finger Nuclease (ZFN) technology to express RFP- and GFP-tagged versions of clathrin and dynamin, respectively, preserving their endogenous

10 Oct 1997: We interpret these results tomean that either Amph1 or Amph2 overexpressionalone leads to sequestration of endogenous dynamininto inactive complexes, but when the two proteins areexpressed together, forming a heterodimer, endocyto-sis

9 May 2003: endogenous proteins and quenching of signals by other cellular proteins. This discussion.

1 Dec 2010: together with endogenous Smc3. Western blotting revealed. that immunoprecipitates of Smc3-HA3 or Smc3AAA-HA3. ... As expected, similar amounts of Smc1-myc9 and endogenous. Smc1 associate with Smc3-HA3.