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The patterned assembly and stepwise Vps4-mediated disassembly of…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Hurtig%202023%20sciadv.ade5224.pdf16 Jul 2023: 1A), hereafter calledVps4E209Q. This point mutation prevents AAA-ATPases like Vps4from hydrolyzing bound ATP and can therefore interfere with theactivity of endogenous Vps4 complexes when overexpressed (24,28). -
doi:10.1016/j.molcel.2004.08.030
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Haering%20Mol%20Cell%202004.pdf20 Sep 2004: Cells separatedsister chromatids with identical kinetics to those thatof the GAL1-10 promoter in cells with an endogenous. ... Nevertheless, noncleavableScc1-HA3 from the endogenous SCC1 promoter. The. sole copy of Cdc20 in all three strains was -
1256917combinedsupplement
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Gligoris-SM.pdf14 Nov 2014: Scc1’s endogenous M1 has been deleted, an N-terminal myc9 epitope was fused in frame and endogenous M30 was changed to I30. ... Endogenous Scc1M102, Smc3M139 and M186 generate a 30 kDa Scc1 and a 5 kDa Smc3 fragment. -
elifesciences.org Szwedziak et al. eLife 2014;3:e04601. DOI:…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/FtsZ_Piotr_eLife_2014.pdf31 Dec 2014: elifesciences.org. Szwedziak et al. eLife 2014;3:e04601. DOI: 10.7554/eLife.04601 1 of 22. Architecture of the ring formed by the tubulin homologue FtsZ in bacterial cell divisionPiotr Szwedziak†, Qing Wang†, Tanmay A M Bharat, Matthew Tsim, Jan -
A positively charged channel within the Smc1/Smc3 hinge required for…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embosmcjingekurze2010.pdf1 Dec 2010: together with endogenous Smc3. Western blotting revealed. that immunoprecipitates of Smc3-HA3 or Smc3AAA-HA3. ... As expected, similar amounts of Smc1-myc9 and endogenous. Smc1 associate with Smc3-HA3. -
Features critical for membrane binding revealed by DivIVA crystal…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/emboj201099a.pdf20 May 2010: tionally complements an endogenous divIVA replacement. This was also seen when sporulation of these strains was. ... endogenous DivIVA through dimerization or oligomerization. When localization of wild-type DivIVA-GFP was analysed in. -
Interaction of tau protein with the dynactincomplex Enrico…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/EMBOJ2007-Magnani-Fan.pdf15 Oct 2007: A) Endogenous p150 co-immunopre-cipitates with tau in differentiated SH-SY5Y cells. Immunoprecipitation was performed with anti-tau antibody BR133 and immunoblots withp150 antibody. ... transgenic mice (data not shown) has been hampered by a. likely -
80096 RPF
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/EMBO2012FtsAPiotr/emboj201276s3.pdf7 Mar 2012: To clarify, we obtained two FtsA crystal structures: a) the first one contained an FtsA dimer (each FtsA molecule had the FtsZ peptide bound and had an endogenous ATP molecule at -
FtsA forms actin-like protofilaments
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/EMBO2012FtsAPiotr/emboj201276a.pdf27 Mar 2012: away from the nucleotide-binding site of FtsA (Figure 2A,. right), which contains an endogenous ATP molecule at. ... appearance that is straightforward to distinguish and. quantify. We then complemented the endogenous ts-FtsA. -
Single‐dose immunisation with a multimerised SARS‐CoV‐2 receptor…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/COVID%20vaccine%202021%201873-3468.14171.pdf29 Aug 2021: Iba1) or 60 min at 37 C (anti-CD3, anti-CD4). This wasfollowed by blocking of endogenous peroxidase (peroxidase.
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