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5 Sep 2003: The resin was washed with buffer Acontaining “front” face. These results suggest that vari-and then buffer B (50 mM Tris, pH 7.5, 200 mM NaCl, and 5 mMous

20 Apr 2002: The cell pellet was resus-pended in 200 l of lysis buffer (1% Triton, 150 mM NaCl, 10 mM Tris,pH 7.4, 1 mM EDTA, 1 mM EGTA, 0.2 ... PLC2 was further purified on a heparin-Sepharose column (Am-ersham Biosciences) followed by gel filtration on a Superdex

1 Dec 2000: The displacement experiment was carried out by mixing 1 mMcontaining 1mM EDTA and 1mM DTT before addition into the assays.PI3K and 2 mM mant-ATP with 50 or 200 mM ... Equilibriumassay with a final ATP concentration of 200 mM with 1.26 mM MgCl2,and

20 Apr 2002: a The wortmannin crystal was soaked in 1 mM wortmannin for 50 min.b The LY294002 crystal was soaked in 1 mM LY294002 for 200 min.c The quercetin crystal had ... Agullo, G., Gamet-Payrastre, L., Manenti, S., Viala, C., Rémésy, C.,[pH 7.2], 200 mM

11 Nov 1999: Ras binding domain Nα1 Nα2 Nα3 Nα4 Rβ1 Rβ2 Rα1. 150 160 170 180 190 200 210 220 230 240 250. ... contain one protein molecule in the asymmetric unit.Diffraction data were collected at ESRF beamlines ID2 and ID14-4 at 100K after freezingcrystals in

6 May 1999: Thus anunfolding of the hinge sub-domain may expose theTolA-binding site on D1 and release the g3pdomains like beads on a string, allowing them tospan a large distance (> 200 AÊ )

1 Apr 2016: Each image of a tube consisted typically of 1200 X 200 pixels ( 5 000 X 1000 A), and was padded to a 1536 X 512 array size, from which Fourier ... Second-order effects, e.g. mul-tiple scattering, were barely detectable in the nega-tive stain study, where

30 Jul 2013: Nicotinic acetylcholinereceptorandthestructuralbasisofneuromuscular transmission:insightsfromTorpedopostsynapticmembranes. Nigel UnwinMRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK. Abstract. The nicotinic

26 Nov 2012: In some experiments of this sort, using a range 0 to 200 V, the potential was found to vary along the thread proportionately to the electron flux. ... manner. The corresponding interference patterns indicate a n ear-zero potential, a high positive

4 May 2004: 510 100 1;0 200 2;0 310 3;0 400 4 ; 0 500.