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23 Jul 2007: 80. 120. 0 50 100 150 200 250. Res. pons. e (R. ... B. 0. 40. 80. 120. 0 100 200 300. Res. pons.

8 Dec 2007: 150. 200. WT E542K E545K Q546K- - - - p85ni. p110 α. ... 0. 50. 100. 150. 200. 250. - - Phosphopeptide. C. B.

14 Jun 2007: 0 50 100 150 200. 0.22. 0.27 27 nM. 61 nM. ... Superdex 200 10/300 column. 16 20128. 6 10 14 18Elution volume (ml).

12 Nov 2006: Briefly, a 1 ml sample of each PX domain was loaded onto a Superdex 200 column (Amersham-Pharmacia) equilibrated and eluted with 20 mM Tris-HCl buffer, pH 7.4, containing

12 Nov 2006: Further purifi cation was by gel fi ltration on Superdex 200 in 10 mM Tris-Cl, pH 8.0, 0.15 M NaCl, 1 mM DTT, and 1 mM EGTA and

14 Jun 2006: 200-fold more potently than p110b. p110a inhibitors. also blocked activation of the mTORC1 pathway as mea-.

18 Apr 2006: D) Gel filtration of full-length ESCRT-I, ESCRT-I (D21-Vps37), and ESCRT-I core on a Superdex 200 HR10/30. ... mM Tris [pH 7.5], 200 mM NaCl and 5 mM b-mercaptoethanol).

1 Nov 2004: on Superdex 200 16/60 equilibrated in buffer C. ... A 200–250 g aliquot of GST-tagged pro-taining 3.1% PEG 35000, 0.1 M Tris acetate (pH 8.5), 1.36 M sodiumtein and 600–700 g of

24 Sep 2004: The high-spin. Acknowledgmentssupernatant was loaded in batch onto Glutathione Sepharose4B.The resin was washed with buffer B (50 mM Tris [pH 7.5] and 200.

6 Oct 2004: indicated dishes were treated with 200 nM wortmannin 40 minRap on ARAP3 Rho GAP activity (Figure 3C).