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Thursday, 21 February 2013, 9.30am-3pm The Old Library, Emmanuel ...
www.memlab.psychol.cam.ac.uk/CAMM/CAMM_programme.pdf19 Feb 2013: We describe a battery of gaze-contingent computerised training programs aimed at training working memory and the endogenous control of attention during infancy. -
Wolbachia Blocks Viral Genome Replication Early in Infection without…
www.jiggins.gen.cam.ac.uk/pdfs/rainey2016.pdf26 Apr 2016: Standard curve anal-ysis was carried out and normalised to an Actin endogenous control. ... Results indicated that when comparedto Actin endogenous control there is 22 times more Wolbachia (S5 Fig), suggesting a densityof at least 100% with more than one -
piRNA pathway is not required for antiviral defense in Drosophila…
www.jiggins.gen.cam.ac.uk/pdfs/petit2016.pdf22 Jul 2016: Never-theless, endogenous piRNAs also have been identified in varioussomatic tissues from fly, mouse, and macaque, as well as in mos-quito head and thorax (22, 23).The piRNA pathway was ... Although one study hasreported the presence of endogenous -
OP-MOLB150120 2111..2129
www.jiggins.gen.cam.ac.uk/pdfs/Palmer2015.pdf10 Nov 2015: endogenous cytokine rather than directlyto pathogen molecules (see below; Leulier and Lemaitre2008). -
msq280 1043..1056
www.jiggins.gen.cam.ac.uk/pdfs/obbard2010mbe.pdf4 Feb 2011: Recent and Recurrent Selective Sweeps of the Antiviral RNAiGene Argonaute-2 in Three Species of Drosophila. Darren J. Obbard,,1,2 Francis M. Jiggins,3 Nicholas J. Bradshaw,4 and Tom J. Little1,2. 1Institute of Evolutionary Biology, University of -
pgen.1000698 1..13
www.jiggins.gen.cam.ac.uk/pdfs/obbard2009plos.pdf30 Oct 2009: Quantifying Adaptive Evolution in the DrosophilaImmune SystemDarren J. Obbard1, John J. Welch1, Kang-Wook Kim1,2, Francis M. Jiggins3. 1 Institute of Evolutionary Biology, University of Edinburgh, Edinburgh, United Kingdom, 2 Department of Animal -
Symbiont strain is the main determinant of variation in…
www.jiggins.gen.cam.ac.uk/pdfs/martinez2017.pdf9 Aug 2017: atpDQALL_R: 50-AATCCTTTATGAGCTTTTGC-30) relative to the. endogenous Drosophila control gene RpL32 (species-specific primers;. ... GGTTT-30; reverse: 50-CTTGAACCATGGCCTTTTG-30) relative to the. endogenous control gene RpL32 (species-specific primers; -
Should Symbionts Be Nice or Selfish? Antiviral Effects of Wolbachia…
www.jiggins.gen.cam.ac.uk/pdfs/martinez2015.pdf2 Jul 2015: For carcasses and testes, the amount of the Wolba-chia gene atpD (atpDQALL_F: 5’-CCTTATCTTAAAGGAGGAAA-3’; atpDQALL_R: 5’-AATCCTTTATGAGCTTTTGC-3’) relative to the endogenous control gene actin 5C (Forwardprimer: -
ppat.1004369 1..13
www.jiggins.gen.cam.ac.uk/pdfs/martinez2014.pdf19 Sep 2014: using the GoScript Reverse Transcription System (Promega) with. random primers. Host gene expression and the Wolbachia densitywere measured relative to the endogenous control gene actin 5C(Table S3) using the SensiFAST -
pgen.1003057 1..11
www.jiggins.gen.cam.ac.uk/pdfs/magwire_2012.pdf16 Nov 2012: As an endogenous control weamplified Actin5c using the primers qActin5c_for2 (59GAGCGC-GGTTACTCTTTCAC 39) and qActin5c_rev2 (59 AAGCCTC-CATTCCCAAGAAC 39).We performed three technical repli-cates of each PCR and -
pgen.1002337 1..11
www.jiggins.gen.cam.ac.uk/pdfs/magwire2011.pdf4 Nov 2011: the endogenous control (actin 5C). The viral titre or geneexpression in resistant flies relative to susceptible flies was. -
OP-VEVO150015 1..12
www.jiggins.gen.cam.ac.uk/pdfs/longdon2015b.pdf18 Nov 2015: 2015; Walker et al. 2015).Furthermore, a number of arthropod genomes contain inte-grated endogenous viral elements (EVEs) with similarity torhabdoviruses, suggesting that these species have been infectedwith rhabdoviruses at some ... intothe insect -
The Causes and Consequences of Changes in Virulence following…
www.jiggins.gen.cam.ac.uk/pdfs/Longdon2015.pdf18 Mar 2015: The viral RNA load was expressedrelative to the endogenous control housekeeping gene RpL32 (Rp49). ... Two qRT-PCR reactions(technical replicates) were carried out per sample with both the viral and endogenous controlprimers. -
pone.0073833 1..6
www.jiggins.gen.cam.ac.uk/pdfs/Longdon2013.pdf16 Jan 2014: an endogenous control reference gene (Elongation factor 1a 100E(EF1a100E)) using a QuantiTect Virus qRT-PCR kit (Qiagen,UK) and Taqman hydrolysis probes (Sigma-Aldrich, UK) on a. ... Relative viral RNA copy. number was then calculated relative to the -
UNCORRECTED PROOF Date: 12:15 Thursday 21 June 2012File:…
www.jiggins.gen.cam.ac.uk/pdfs/longdon2012b.pdf10 Aug 2012: UNCORRECTED PROOF Date: 12:15 Thursday 21 June 2012File: Rhabdoviruses 3P. 8The Sigma Viruses of DrosophilaBen Longdon, Lena Wilfert and Francis M. Jiggins. AbstractThe sigma virus of Drosophila melanogaster (DMelSV) was discovered accidentally over -
ppat.1002260 1..9
www.jiggins.gen.cam.ac.uk/pdfs/longdon2011c.pdf26 Sep 2011: The copy-number of viral genomic RNA was expressed. relative to the endogenous control housekeeping gene RpL32 (Rp49).We designed different RpL32 primers specific for each species. ... technical replicates) were carried out per sample with both the. -
Biol. Lett. doi:10.1098/rsbl.2011.0160 Published online Evolutionary…
www.jiggins.gen.cam.ac.uk/pdfs/longdon2011b.pdf18 Jul 2011: J. 2010 Endogenous viralelements in animal genomes. PLOS Genet. 6,e1001191. (doi:10.1371/Journal.Pgen.1001191). -
genet127696 141..150
www.jiggins.gen.cam.ac.uk/pdfs/Longdon2011.pdf11 May 2011: RNA was extractedand reverse-transcribed (see above), and qRT-PCR was used tomeasure the viral titer relative to an endogenous control(RpL32) using the delta delta CT method. -
pgen.1008864
www.jiggins.gen.cam.ac.uk/pdfs/lewis2020.pdf22 Apr 2021: RESEARCH ARTICLE. Widespread conservation and lineage-specificdiversification of genome-wide DNAmethylation patterns across arthropodsSamuel H. Lewis1,2,3, Laura RossID. 4, Stevie A. BainID4, Eleni PahitaID. 2,3, StephenA. SmithID. 5, Richard -
Pan-arthropod analysis reveals somatic piRNAs as an ancestral defence …
www.jiggins.gen.cam.ac.uk/pdfs/lewis2017.pdf5 Dec 2017: Virus identification and genome assembly. To identify viruses, we first mapped RNA-Seq reads to the genome of the host species to exclude genome-derived transcripts, thus filtering out endogenous viral ... Nat. Commun. 7, 13856 (2016). 9. Ghildiyal, M. -
hered master 8..hered 357 .. Page87
www.jiggins.gen.cam.ac.uk/pdfs/Heredity1998.pdf3 Nov 2006: No successful treatmentsof normal sex ratio matrilines were completed tocontrol for the endogenous effects of the antibioticon sex ratio, although none is likely. -
15566153538820 1..21
www.jiggins.gen.cam.ac.uk/pdfs/duxbury2019.pdf30 Apr 2019: endogenous control primers. Each qRT-PCR plate contained three standard samples, and all experi-. ... of the virus and the endogenous control. Viral load calculated without using the endogenous control. -
Cogni_et_al-2021-Communications_Biology
www.jiggins.gen.cam.ac.uk/pdfs/cogni2021.pdf25 Nov 2021: Because primers for the viruses and the endogenous genes haveapproximate similar efficiencies, we calculated viral titer from the cycle thresholds(Ct) as 2ΔCt, where ΔCt = CtRpL32 Ctvirus. -
pgen.1003896 1..22
www.jiggins.gen.cam.ac.uk/pdfs/Chrostek2013.pdf16 Jan 2014: Wolbachia Variants Induce Differential Protection toViruses in Drosophila melanogaster: A Phenotypic andPhylogenomic AnalysisEwa Chrostek1, Marta S. P. Marialva1, Sara S. Esteves1, Lucy A. Weinert2, Julien Martinez3,. Francis M. Jiggins3, Luis -
1471-2156-10-75.fm
www.jiggins.gen.cam.ac.uk/pdfs/Carpenter2009b.pdf22 Feb 2010: Journalof Neurogenetics 1998, 12(4):227-240. 12. Kawamura Y, Saito K, Kin T, Ono Y, Asai K, Sunohara T, Okada TN,Siomi MC, Siomi H: Drosophila endogenous small RNAs bind -
pone.0006838 1..11
www.jiggins.gen.cam.ac.uk/pdfs/Carpenter2009.pdf18 Sep 2009: 20], but not in our experiments. As an endogenous control,. we also measured the expression of RpL32 (Rp49). ... Tsai et al. [20], we used Act88F as an endogenous control. -
A Polymorphism in the Processing Body Component Ge-1 Controls…
www.jiggins.gen.cam.ac.uk/pdfs/cao2015.pdf24 Jan 2016: RESEARCH ARTICLE. A Polymorphism in the Processing BodyComponent Ge-1 Controls Resistance toa Naturally Occurring Rhabdovirus inDrosophilaChuan Cao, Michael M. Magwire¤a, Florian Bayer¤b, Francis M. Jiggins. Department of Genetics, University of -
PNAS202122026_proof
www.jiggins.gen.cam.ac.uk/pdfs/brosh2022.pdf24 Aug 2022: In mammals, TEs and endogenous retroviruses have playedan important role in the evolution of the immune system, giving rise to enhancers regu-lating the expression of genes in response to interferon, ... Chuong, N. C. Elde, C. Feschotte, Regulatory -
flm466.dvi
www.itg.cam.ac.uk/people/heh/Paper202.pdf8 May 2007: 1993 Endogenous growth of persistently activevolcanoes. Nature 366, 554–557. Happel, J. & -
Dr John W Wills | Girton College
www.girton.cam.ac.uk/people/dr-john-w-willsI am currently focussed on demonstrating the capabilities of this approach by investigating the roll of newly-discovered, endogenous gut nanomineral particles in shuttling antigen, promoting oral immuno-tolerance and how -
Development/Plasticity/Repair Developmental Modulation of Retinal…
www.damtp.cam.ac.uk/user/sje30/papers/sernagor2003_jn.pdf14 Jun 2014: Firstwe blocked endogenous GABA with theGABAA antagonist bicuculline (1–10 M)at various developmental stages. -
PII: S1350-9462(00)00024-0
www.damtp.cam.ac.uk/user/sje30/papers/sernagor2001.pdf14 Jun 2014: PII: S1350-9462(00)00024-0. Development of Retinal Ganglion Cell Structure and Function. Evelyne Sernagor, Stephen J. Eglen and Rachel O. L. WongDepartment of Neurobiology, Medical School, University of Newcastle upon Tyne, Framlington -
Determinants of the Exclusion Zone inDopaminergic Amacrine Cell…
www.damtp.cam.ac.uk/user/sje30/papers/raven2003.pdf14 Jun 2014: Determinants of the Exclusion Zone inDopaminergic Amacrine Cell Mosaics. MARY A. RAVEN,1 STEPHEN J. EGLEN,2 JOHN J. OHAB,1. AND BENJAMIN E. REESE11Neuroscience Research Institute and Department of Psychology, University of California. at Santa -
TH EJ OU RN AL OF CE LL BIO ...
www.damtp.cam.ac.uk/user/sje30/papers/kishi2005.pdf14 Jun 2014: The fusion proteinwas associated with AChR aggregates when it was expressed atmodest levels (unpublished data; levels assessed by stainingwith anti-LL5, and comparing to endogenous levels in con-trols). ... Only the brightest myotubes wouldappear -
dql003
www.damtp.cam.ac.uk/user/sje30/papers/eglen2006.pdf14 Jun 2014: Mathematical Medicine and Biology (2006) 23, 7999doi:10.1093/imammb/dql003Advance Access publication on March 1, 2006. Development of regular cellular spacing in the retina: theoretical models. STEPHEN J. EGLEN†. Department for Applied Mathematics -
PII: S0896-6273(03)00808-0
www.damtp.cam.ac.uk/user/sje30/papers/eglen2003_neuron.pdf14 Jun 2014: Also, despite the usefulness of the 2 KO animal, a transgenic animals in which endogenous activity pat-terns are altered has enabled McLaughlin et al. -
10891_ftp
www.damtp.cam.ac.uk/user/sje30/papers/eglen2003.pdf14 Jun 2014: Dopaminergic Amacrine Cells in theInner Nuclear Layer and Ganglion. Cell Layer Comprise a SingleFunctional Retinal Mosaic. STEPHEN J. EGLEN,1 MARY A. RAVEN,2 ERIC TAMRAZIAN,2. AND BENJAMIN E. REESE21Department of Anatomy and Neurobiology, Washington -
Reconstruction Algorithms for PositronEmission Tomography and Single…
www.damtp.cam.ac.uk/user/na/NA_papers/NA2004_07.pdf11 Oct 2004: 6, 933 (2000). [16] L.A. Green et al., Indirect Monitoring of Endogenous Gene Expression by PositronEmission Tomography (PET) Imaging of Reporter Gene Expression in TransgenicMice, Mol. -
Fluid Mechanics of Mosaic Ciliated Tissues
www.damtp.cam.ac.uk/user/lauga/papers/204.pdf29 Nov 2021: While the flowdriven by a single such vortex decays quickly with distancefrom the skin, we show that long range contributions ofother bundles slows the decay of this endogenous flow anddetermines the ... Lateralvelocity at (e) x; y ð0; 0Þ and (f) ð40 -
on November 22, 2017http://rsif.royalsocietypublishing.org/Downloaded …
www.damtp.cam.ac.uk/user/lauga/papers/142.pdf22 Nov 2017: Direct visualizations [6] ofthe streaming flows (by means of endogenous tracer particles) and the micro-tubules (fluorescently labelled) show that the flows are disordered on the scaleof the oocyte and are -
THE ROTARY MOTOR OF BACTERIAL FLAGELLA
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/membrane_proteins/bergreview.pdf27 Nov 2011: One can polymerize flagellin onto FlgL in such mutants by adding it exogenously(151, 152) or grow filaments in the normal fashion by supplementing suchmutants, either with endogenous FliD (153) or ... strain V4051 (170), a peritrichously flagellated, -
CURRENT TOPICS IN PLANT RESEARCH The sliding theory of ...
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/FDSE/Shimmen07.pdf2 Sep 2012: Endogenous myosin isinactivated by N-ethylmaleimide (NEM) treatment and effusesout by strong perfusion (d). -
Chlamy_science_full
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/FDSE/Polinetal09.pdf31 Jul 2009: symptoms. Ligands of the translocator protein[18 kilodaltons (kD)] may promote the synthesis of endogenous neurosteroids, which also exertanxiolytic effects in animal models. ... Ring A–reducedneurosteroids are endogenous metabolites ofthe hormone -
on September 22,…
www.damtp.cam.ac.uk/user/gold/pdfs/silicachoano_jrsi.pdf22 Sep 2016: This sensing system must be endogenous tothe cell rather than being controlled by extracellular conditions(because sufficient Si is present for costal strip formation), and isunlikely to be transporter-based because -
201203575 15109..15114
www.damtp.cam.ac.uk/user/gold/pdfs/oocyte_streaming.pdf19 Sep 2012: Development132:3743–3752. 8. Forrest KM, Gavis ER (2003) Live imaging of endogenous mRNA reveals a diffusion andentrapment mechanism for nanos mRNA localization in Drosophila. -
Fluid Mechanics of Mosaic Ciliated Tissues
www.damtp.cam.ac.uk/user/gold/pdfs/mosaic.pdf2 Nov 2021: While the flowdriven by a single such vortex decays quickly with distancefrom the skin, we show that long range contributions ofother bundles slows the decay of this endogenous flow anddetermines the ... Lateralvelocity at (e) x; y ð0; 0Þ and (f) ð40 -
Chlamydomonas Swims with Two “Gears” in a Eukaryotic Version of…
www.damtp.cam.ac.uk/user/gold/pdfs/gears.pdf21 May 2023: symptoms. Ligands of the translocator protein[18 kilodaltons (kD)] may promote the synthesis of endogenous neurosteroids, which also exertanxiolytic effects in animal models. ... Ring A–reducedneurosteroids are endogenous metabolites ofthe hormone -
on November 22, 2017http://rsif.royalsocietypublishing.org/Downloaded …
www.damtp.cam.ac.uk/user/gold/pdfs/filament_oscillations.pdf22 Nov 2017: Direct visualizations [6] ofthe streaming flows (by means of endogenous tracer particles) and the micro-tubules (fluorescently labelled) show that the flows are disordered on the scaleof the oocyte and are -
15081476132025 1..25
www.damtp.cam.ac.uk/user/gold/pdfs/dynactin.pdf16 Oct 2017: phenotype. (D–E) Confocal images of fluorescent in situ hybridisations (FISH) to endogenous oskar mRNA in a. -
ELIFE06088 1..31
www.damtp.cam.ac.uk/user/gold/pdfs/Drosophila_axis.pdf23 Sep 2015: protein Exuperantia. Endogenous bicoid mRNA can be approximated by so-called conditioned bicoid. ... In the model, starting from an initial. distribution at the anterior surface where endogenous bicoid mRNA enters the oocyte, cargo quickly.
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