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ppat.1002260 1..9
www.jiggins.gen.cam.ac.uk/pdfs/longdon2011c.pdf26 Sep 2011: The copy-number of viral genomic RNA was expressed. relative to the endogenous control housekeeping gene RpL32 (Rp49).We designed different RpL32 primers specific for each species. ... technical replicates) were carried out per sample with both the. -
Biol. Lett. doi:10.1098/rsbl.2011.0160 Published online Evolutionary…
www.jiggins.gen.cam.ac.uk/pdfs/longdon2011b.pdf18 Jul 2011: J. 2010 Endogenous viralelements in animal genomes. PLOS Genet. 6,e1001191. (doi:10.1371/Journal.Pgen.1001191). -
genet127696 141..150
www.jiggins.gen.cam.ac.uk/pdfs/Longdon2011.pdf11 May 2011: RNA was extractedand reverse-transcribed (see above), and qRT-PCR was used tomeasure the viral titer relative to an endogenous control(RpL32) using the delta delta CT method. -
pgen.1008864
www.jiggins.gen.cam.ac.uk/pdfs/lewis2020.pdf22 Apr 2021: RESEARCH ARTICLE. Widespread conservation and lineage-specificdiversification of genome-wide DNAmethylation patterns across arthropodsSamuel H. Lewis1,2,3, Laura RossID. 4, Stevie A. BainID4, Eleni PahitaID. 2,3, StephenA. SmithID. 5, Richard -
Pan-arthropod analysis reveals somatic piRNAs as an ancestral defence …
www.jiggins.gen.cam.ac.uk/pdfs/lewis2017.pdf5 Dec 2017: Virus identification and genome assembly. To identify viruses, we first mapped RNA-Seq reads to the genome of the host species to exclude genome-derived transcripts, thus filtering out endogenous viral ... Nat. Commun. 7, 13856 (2016). 9. Ghildiyal, M. -
hered master 8..hered 357 .. Page87
www.jiggins.gen.cam.ac.uk/pdfs/Heredity1998.pdf3 Nov 2006: No successful treatmentsof normal sex ratio matrilines were completed tocontrol for the endogenous effects of the antibioticon sex ratio, although none is likely. -
15566153538820 1..21
www.jiggins.gen.cam.ac.uk/pdfs/duxbury2019.pdf30 Apr 2019: endogenous control primers. Each qRT-PCR plate contained three standard samples, and all experi-. ... of the virus and the endogenous control. Viral load calculated without using the endogenous control. -
Cogni_et_al-2021-Communications_Biology
www.jiggins.gen.cam.ac.uk/pdfs/cogni2021.pdf25 Nov 2021: Because primers for the viruses and the endogenous genes haveapproximate similar efficiencies, we calculated viral titer from the cycle thresholds(Ct) as 2ΔCt, where ΔCt = CtRpL32 Ctvirus. -
pgen.1003896 1..22
www.jiggins.gen.cam.ac.uk/pdfs/Chrostek2013.pdf16 Jan 2014: Wolbachia Variants Induce Differential Protection toViruses in Drosophila melanogaster: A Phenotypic andPhylogenomic AnalysisEwa Chrostek1, Marta S. P. Marialva1, Sara S. Esteves1, Lucy A. Weinert2, Julien Martinez3,. Francis M. Jiggins3, Luis -
1471-2156-10-75.fm
www.jiggins.gen.cam.ac.uk/pdfs/Carpenter2009b.pdf22 Feb 2010: Journalof Neurogenetics 1998, 12(4):227-240. 12. Kawamura Y, Saito K, Kin T, Ono Y, Asai K, Sunohara T, Okada TN,Siomi MC, Siomi H: Drosophila endogenous small RNAs bind
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