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room temperature for 20 min in glass-well plate on nutator SNAP-tag / CLIP-tag: no change in labeling intensity after prolonged fixation of up to 60 min. ... image ===cell-permeable substrates=== dissect brains in ice-cold 0.1 M PB fixation in 4% PFA (in

Protocol for chemical labeling of mouse brain slices===== ===Vibratome slices (200 µm)=== fix slices in 4% PFA (in 0.1 M PB) at room temperature for 20 min in glass-well

Notes on substrates for chemical labeling===== store substrates at -20C in presence of dessicant this avoids hydrolysis of substrates for substrates that are purchased in dry powder form: dissolve in fresh, water-free DMSO using fresh DMSO from a

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There are 8 x 20 way IDC connectors each of which can control 8 valve pairs. ... Complete rig. Log In. alex/projects/odour_presentation_system/start.txt. Last modified: 2021/06/18 20:33 by. jefferis.

These pages describe the supplemental data and software available online or via >=1.5Tb external hard drive to accompany:. Cachero S., Ostrovsky A.D., Yu J.Y., Dickson B.J., and Jefferis G.S.X.E. Sexual Dimorphism in the Fly Brain. Current Biology

si/frulhns/start.txt. Last modified: 2019/06/20 08:53 by. jefferis. Except where otherwise noted, content on this wiki is licensed under the following license:.

Please seeLog In. si/kohl2014/start.txt. Last modified: 2014/11/13 20:23 by. jefferis. Except where otherwise noted, content on this wiki is licensed under the following license:.

dissect brains in ice-cold 0.1 M PB. fixation in 4% PFA (in 0.1 M PB) at room temperature for 20 min in glass-well plate on nutator. ... fixation in 4% PFA (in 0.1 M PB) at room temperature for 20 min in glass-well plate on nutator.

fix slices in 4% PFA (in 0.1 M PB) at room temperature for 20 min in glass-well plate on nutator.