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https://www2.mrc-lmb.cam.ac.uk/microscopes4schools/downloads/protocols
https://www2.mrc-lmb.cam.ac.uk/microscopes4schools/downloads/M4S_activities.pdf11 Mar 2022: Water flea Daphnia pulex. Materials. - glass microscope slides with pre-prepared chamber of electrical tape- plastic cover slips- paper towels or tissue- plastic pipette or dropper- Daphnia pulex collected from a pond/stream or acquired commercially- -
KWRM_A_992668_O
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/2015-3.pdf20 Oct 2015: Download by: [82.6.6.247] Date: 20 October 2015, At: 11:07. Worm. ISSN: (Print) 2162-4054 (Online) Journal homepage: http://www.tandfonline.com/loi/kwrm20. -
Name: than you think! SafetyOur worms, and the bacteria ...
https://www2.mrc-lmb.cam.ac.uk/groups/worms/download/Worms-are-Cleverer-workbook.pdf18 Jan 2018: A. @MRC_LMB (MRC.LMB). using #LMBworms. Big Biology cover download(1). Meet-our-worms download(1). ... Using-senses download(1). Old-worms download(1). -
Worms are Cleverer than you think! Notes for activities ...
https://www2.mrc-lmb.cam.ac.uk/groups/worms/download/Notes-for-activities-PDF.pdf19 Jan 2018: Worms are Cleverer than you think! Notes for activities. We all appreciate how cool worms are and how powerful they are as a model organism, but. how do we communicate this to the general public? This was our starting point when we decided to design -
Dbouk 1..13
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/vadas_science_signaling.pdf5 Dec 2012: 253), ra89. [DOI: 10.1126/scisignal.2003264] 5Science SignalingRoger L. Williams and Jonathan M. Backer (4 December 2012) Harden, Alan V. Smrcka, Ronald Taussig, Anne R. Bresnick, Bernd Nürnberg, Christine Hsueh, Olga Perisic, Christian Harteneck, -
PII: S1097-2765(03)00356-3
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/gripdomain.pdf27 Oct 2003: Molecular Cell, Vol. 12, 863–874, October, 2003, Copyright 2003 by Cell Press. Structural Basis for Arl1-Dependent Targeting ofHomodimeric GRIP Domains to the Golgi Apparatus. elle (Donaldson and Jackson, 2000). For example, Arf1recruits COP I -
Calcium-dependent Membrane Penetration Is a Hallmark of theC2 Domain…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9668093.pdf20 Apr 2002: Calcium-dependent Membrane Penetration Is a Hallmark of theC2 Domain of Cytosolic Phospholipase A2 Whereas the C2A Domainof Synaptotagmin Binds Membranes Electrostatically. (Received for publication, May 21, 1998). Bazbek Davletov‡, Olga Perisic, -
Catalytic Domain of Phosphoinositide-specificPhospholipase C…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9565585.pdf20 Apr 2002: Catalytic Domain of Phosphoinositide-specificPhospholipase C (PLC)MUTATIONAL ANALYSIS OF RESIDUES WITHIN THE ACTIVE SITE AND HYDROPHOBIC RIDGE OF PLCd1. (Received for publication, November 20, 1997, and in revised form, February 18, 1998). Moira V. -
Structural and Mechanistic Comparison of Prokaryotic and Eukaryotic…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9466937.pdf15 Jan 1998: Structural and Mechanistic Comparison of Prokaryoticand Eukaryotic Phosphoinositide-specificPhospholipases C. Dirk W. Heinz1, Lars-Oliver Essen2 and Roger L. Williams3. 1Institut fuÈ r OrganischeChemie und BiochemieUniversitaÈt -
Crystal Structure of a Calcium-Phospholipid Binding Domainfrom…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9430701.pdf20 Apr 2002: Crystal Structure of a Calcium-Phospholipid Binding Domainfrom Cytosolic Phospholipase A2. (Received for publication, September 24, 1997). Olga Perisic‡, Sun Fong, Denise E. Lynch‡, Mark Bycroft, and Roger L. Williams‡. From the ‡Medical -
Phosphoinositide-Specific Phospholipase Cδ1 Activity toward Micellar…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9287165.pdf20 Apr 2002: Phosphoinositide-Specific Phospholipase Cδ1 Activity toward Micellar Substrates,Inositol 1,2-Cyclic Phosphate, and Other Water-Soluble Substrates: A Sequential. Mechanism and Allosteric Activation†. Yiqin Wu,‡ Olga Perisic, Roger L. Williams, -
Proc. Natl. Acad. Sci. USAVol. 94, pp. 9637–9642, September ...
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9275175.pdf20 Apr 2002: Proc. Natl. Acad. Sci. USAVol. 94, pp. 9637–9642, September 1997Biophysics. The 2.0-Å resolution crystal structure of a trimeric antibodyfragment with noncognate VH–VL domain pairs shows arearrangement of VH CDR3XUE Y. PEI, PHILIPP HOLLIGER, -
A Ternary Metal Binding Site in the C2 Domain ...
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9062102.pdf20 Apr 2002: A Ternary Metal Binding Site in the C2 Domain of Phosphoinositide-SpecificPhospholipase C-δ1†,‡. Lars-Oliver Essen,,| Olga Perisic, Denise E. Lynch, Matilda Katan, and Roger L. Williams,. Centre for Protein Engineering, MRC Centre, Hills Road, -
Structural Mapping of the Catalytic Mechanism for a…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9048554.pdf20 Apr 2002: Structural Mapping of the Catalytic Mechanism for a MammalianPhosphoinositide-Specific Phospholipase C†,‡. Lars-Oliver Essen,,| Olga Perisic, Matilda Katan, Yiqin Wu, Mary F. Roberts, and Roger L. Williams,. Centre for Protein Engineering, MRC -
pnas201206839 17424..17429
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/23045692.pdf21 Nov 2012: ESCRT-III binding protein MITD1 is involved incytokinesis and has an unanticipated PLD foldthat binds membranesMichael A. Haddersa,1, Monica Agromayorb,2,1, Takayuki Obitaa,3, Olga Perisica, Anna Caballeb, Magdalena Klocb,Meindert H. Lamersa, Roger -
pnas201205508 15259..15264
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/22949682.pdf25 Nov 2012: Oncogenic mutations mimic and enhance dynamicevents in the natural activation of phosphoinositide3-kinase p110α (PIK3CA)John E. Burke, Olga Perisic, Glenn R. Masson, Oscar Vadas, and Roger L. Williams1. Laboratory of Molecular Biology, Medical -
Regulation of lipid binding underlies the activation mechanism of…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/22120714.pdf21 Nov 2012: Oncogene 31, 3655 (2012). doi:10.1038/onc.2011.532 -
www.landesbioscience.com Autophagy 805 Autophagy 6:6, 805-807; August …
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/20574157.pdf2 Aug 2010: www.landesbioscience.com Autophagy 805. Autophagy 6:6, 805-807; August 16, 2010; 2010 Landes BioscienceAUTOPHAGIC PUNCTUM AUTOPHAGIC PUNCTUM. Key words: Vps34, PI 3-kinase, struc-ture, inhibitor, enzyme, autophagy, Vps15, PtdIns3P, phosphoinositide. -
DOI: 10.1126/science.1184429 , 1638 (2010); 327Science et al.Simon…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/20339072.pdf2 Aug 2010: DOI: 10.1126/science.1184429 , 1638 (2010); 327Science. et al.Simon Miller,the Structure of the Lipid Kinase Vps34Shaping Development of Autophagy Inhibitors with. This copy is for your personal, non-commercial use only. clicking herecolleagues, -
©20 10 N atur e A mer ica, Inc. ...
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/20081827.pdf2 Aug 2010: 20. 10 N. atur. e A. mer. ica,. Inc. All. righ. ts r. eser. ved. NATURE CHEMICAL BIOLOGY | VOL 6 | FEBRUARY 2010 | www.nature.com/naturechemicalbiology 1 1 7. ARTICLEPUBLISHED ONLINE: 10 JANUARY 2010 | DOI: 10.1038/NCHEMBIO.293. The PI(3)Ks are -
bst641-09a
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/19614567.pdf2 Aug 2010: Morton. Lecture. Morton LectureForm and flexibility in phosphoinositide 3-kinasesRoger Williams1, Alex Berndt, Simon Miller, Wai-Ching Hon and Xuxiao ZhangMRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, U.K. AbstractPI3Ks -
DOI: 10.1126/science.1165322 , 1710 (2008); 322Science et al.Rachel…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/19008417.pdf18 Dec 2008: DOI: 10.1126/science.1165322 , 1710 (2008); 322Science. et al.Rachel Y. Samson,ArchaeaA Role for the ESCRT System in Cell Division in. www.sciencemag.org (this information is current as of December 18, 2008 ):The following resources related to this -
npgrj_nchembio_117 691..699
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/18849971.pdf16 Oct 2008: Targeted polypharmacology: discovery of dual inhibitorsof tyrosine and phosphoinositide kinasesBeth Apsel1, Jimmy A Blair2, Beatriz Gonzalez3,6, Tamim M Nazif4, Morri E Feldman1, Brian Aizenstein5,Randy Hoffman5, Roger L Williams3, Kevan M Shokat2,4 -
doi:10.1016/j.str.2008.06.010
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/18786397.pdf3 Sep 2008: Structure. Article. Structure and Disassembly of FilamentsFormed by the ESCRT-III Subunit Vps24Sara Ghazi-Tabatabai,1 Suraj Saksena,2 Judith M. Short,1 Ajaybabu V. Pobbati,1 Dmitry B. Veprintsev,1. R. Anthony Crowther,1 Scott D. Emr,2 Edward H. -
LETTERS Structural basis for selective recognition of ESCRT-IIIby the …
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17928861.pdf8 Dec 2007: LETTERS. Structural basis for selective recognition of ESCRT-IIIby the AAA ATPase Vps4Takayuki Obita1, Suraj Saksena2, Sara Ghazi-Tabatabai1, David J. Gill1, Olga Perisic1, Scott D. Emr2. & Roger L. Williams1. The AAA1 ATPases are essential for -
The structural basis of novel endosome anchoringactivity of KIF16B ...
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17641687.pdf23 Jul 2007: The structural basis of novel endosome anchoringactivity of KIF16B kinesin. Nichole R Blatner1, Michael I Wilson2,Cai Lei3, Wanjin Hong3, Diana Murray4,Roger L Williams2 and Wonhwa Cho 1,1Department of Chemistry, University of Illinois at Chicago, -
DOI: 10.1126/science.1135394 , 239 (2007); 317Science et al.Nabil…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17626883.pdf8 Dec 2007: DOI: 10.1126/science.1135394 , 239 (2007); 317Science. et al.Nabil Miled,the Phosphoinositide 3-Kinase Catalytic SubunitMechanism of Two Classes of Cancer Mutations in. www.sciencemag.org (this information is current as of December 8, 2007 ):The -
Structural and Membrane Binding Analysis of the PhoxHomology Domain…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17581820.pdf8 Dec 2007: Structural and Membrane Binding Analysis of the PhoxHomology Domain of Bem1pBASIS OF PHOSPHATIDYLINOSITOL 4-PHOSPHATE SPECIFICITYSReceived for publication, April 4, 2007, and in revised form, May 29, 2007 Published, JBC Papers in Press, June 20, 2007 -
Tumour prevention by a single antibody domaintargeting the…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17568777.pdf22 Jul 2007: Tumour prevention by a single antibody domaintargeting the interaction of signal transductionproteins with RAS. Tomoyuki Tanaka, Roger L Williamsand Terence H Rabbitts. MRC Laboratory of Molecular Biology, Hills Road, Cambridge, UK. Many -
nrm2162.indd
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17450176.pdf14 Jun 2007: Many plasma-membrane proteins are in a constant flux throughout the internal trafficking pathways of the cell. Some receptors, such as that for transferrin, are continuously internalized into recycling endosomes and returned to the cell surface. -
25 Structural studies of phosphoinositide 3-kinase-dependent traffi c …
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17233579_proof.pdf14 Jun 2007: 25. Structural studies of phosphoinositide 3-kinase-dependent traffi c to multivesicular bodiesDavid J. Gill1, Hsiangling Teo1, Ji Sun‡, Olga Perisic, Dmitry B. Veprintsev†, Yvonne Vallis, Scott D. Emr‡ and Roger L. Williams2. MRC Laboratory -
Structural insight into the ESCRT-I/-II link and itsrole in ...
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17215868.pdf14 Jun 2007: Structural insight into the ESCRT-I/-II link and itsrole in MVB trafficking. David J Gill1,, Hsiangling Teo1, Ji Sun2,Olga Perisic1, Dmitry B Veprintsev3,Scott D Emr2 and Roger L Williams1. 1MRC Laboratory of Molecular Biology, Medical Research -
M6_07079a2
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17038310.pdf12 Nov 2006: Stahelin et al. 1. Structural and Membrane Binding Analysis of the PX Domain of Phosphoinositide 3-Kinase-C2α. Robert V. Stahelin1, Dimitrios Karathanassis2,5, Karol S. Bruzik3, Michael D. Waterfield4, Jerónimo. Bravo2,6, Roger L. Williams2 and -
TH EJ OU RN AL OF CE LL BIO ...
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/16785321.pdf12 Nov 2006: TH. EJ. OU. RN. AL. OF. CE. LL. BIO. LO. GY. JCB: ARTICLE. The Rockefeller University Press $8.00The Journal of Cell Biology, Vol. 173, No. 6, June 19, 2006 867–877http://www.jcb.org/cgi/doi/10.1083/jcb.200603153 JCB 867. IntroductionCentrins are -
doi:10.1016/j.cell.2006.03.035
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/16647110.pdf14 Jun 2006: A Pharmacological Map of the PI3-KFamily Defines a Role for p110ain Insulin SignalingZachary A. Knight,1 Beatriz Gonzalez,4,7 Morri E. Feldman,1 Eli R. Zunder,1 David D. Goldenberg,2. Olusegun Williams,1 Robbie Loewith,5 David Stokoe,3 Andras -
doi:10.1016/j.cell.2006.01.047
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/16615893.pdf18 Apr 2006: ESCRT-I Core and ESCRT-II GLUEDomain Structures Reveal Role for GLUEin Linking to ESCRT-I and MembranesHsiangling Teo,1,4, David J. Gill,1,4 Ji Sun,3 Olga Perisic,1 Dmitry B. Veprintsev,2 Yvonne Vallis,1. Scott D. Emr,3 and Roger L. Williams1,1 MRC -
doi:10.1016/j.coph.2005.03.002
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/15963759.pdf12 Nov 2006: Phosphoinositide 3-kinases as drug targets in cancerLen Stephens1, Roger Williams2 and Phillip Hawkins1. The past two years have seen phosphoinositide 3-kinases. (PI3Ks) move from being seen as potential targets for. chemotherapeutics, to one of -
doi:10.1016/j.devcel.2004.09.003
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/15469844.pdf1 Nov 2004: Developmental Cell, Vol. 7, 559–569, October, 2004, Copyright 2004 by Cell Press. ESCRT-II, an Endosome-Associated Complex Requiredfor Protein Sorting: Crystal Structure andInteractions with ESCRT-III and Membranes. Ten of these proteins are -
doi:10.1016/j.molcel.2004.08.004
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/15350214.pdf24 Sep 2004: Molecular Cell, Vol. 15, 689–701, September 10, 2004, Copyright 2004 by Cell Press. Structure of a Human Inositol 1,4,5-Trisphosphate3-Kinase: Substrate Binding RevealsWhy It Is Not a Phosphoinositide 3-Kinase. which includes InsP6 kinases -
doi:10.1016/j.cub.2004.07.058
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/15296756.pdf6 Oct 2004: Current Biology, Vol. 14, 1380–1384, August 10, 2004, 2004 Elsevier Ltd. All rights reserved. DOI 10.1016/j. c u b. 2 0 0 4. 0 7. 0 5 8. ARAP3 Is a PI3K- and Rap-Regulated GAP for RhoA. Further titration of the relative levels of ARAP3 andSonja -
Structural Insights into Endosomal Sorting Complex Required…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/15044434.pdf24 Sep 2004: Structural Insights into Endosomal Sorting Complex Required forTransport (ESCRT-I) Recognition of Ubiquitinated Proteins. Received for publication, January 5, 2004, and in revised form, March 22, 2004Published, JBC Papers in Press, March 24, 2004, -
doi:10.1016/S1097-2765(03)00246-6
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/12887891.pdf5 Sep 2003: Molecular Cell, Vol. 12, 39–50, July, 2003, Copyright 2003 by Cell Press. PB1 Domain-Mediated Heterodimerizationin NADPH Oxidase and Signaling Complexesof Atypical Protein Kinase C with Par6 and p62. al, 2003). The molecular organization of -
cdf519 5057..5068
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/12356722.pdf12 Oct 2002: Dimitrios Karathanassis1,Robert V.Stahelin2, Jero nimo Bravo1,3,Olga Perisic1, Christine M.Pacold1,Wonhwa Cho2 and Roger L.Williams1,4. 1MRC Laboratory of Molecular Biology, Hills Road,Cambridge CB2 2QH, UK and 2Department of Chemistry,University -
PII: S1097-2765(01)00372-0
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/11684018.pdf20 Apr 2002: Molecular Cell, Vol. 8, 829–839, October, 2001, Copyright 2001 by Cell Press. The Crystal Structure of the PX Domain from p40phoxBound to Phosphatidylinositol 3-Phosphate. Vam7 (Cheever et al., 2001), SNX3 (Xu et al., 2001b),and p40 phox -
Tyrosine Residues in Phospholipase C�2 Essential for the…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/11606584.pdf20 Apr 2002: Tyrosine Residues in Phospholipase C2 Essential for the EnzymeFunction in B-cell Signaling. Received for publication, August 8, 2001, and in revised form, October 9, 2001Published, JBC Papers in Press, October 17, 2001, DOI 10.1074/jbc.M107577200. -
x96411u107
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/11136978.pdf1 Dec 2000: Cell, Vol. 103, 931–943, December 8, 2000, Copyright 2000 by Cell Press. Crystal Structure and Functional Analysisof Ras Binding to Its EffectorPhosphoinositide 3-Kinase g. suppression of apoptosis (Franke et al., 1997), reorgani-zation of the -
x96255u149
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/11090628.pdf20 Apr 2002: Molecular Cell, Vol. 6, 909–919, October, 2000, Copyright 2000 by Cell Press. Structural Determinants of Phosphoinositide3-Kinase Inhibition by Wortmannin, LY294002,Quercetin, Myricetin, and Staurosporine. for proteins such as protein kinase B -
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https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/10856244.pdf6 Jun 2000: "# $. %! & ' () & ,! & ,! " # $ % # & # ' # & & & & # '! - - - -. ,! / 0012+ 3! )! , , 4! , / 005 002+ 3! /62! 3! 78& & &78& & /9) 00%2+ 3! &! & ' /! 82 - /! 8%2+ 8 &! : ,)! )! 78! &! & 3! )! & 4 ; & 9 /2 ,)! & 6 )! )! & /9) 00%2+ ) )! < /= 00>2+. -
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https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/10580505.pdf11 Nov 1999: 1999 Macmillan Magazines Ltd. letters to nature. NATURE | VOL 402 | 18 NOVEMBER 1999 | www.nature.com 313. 12. Macleod, K. F. et al. p53-dependent and independent expression of p21 during cell growth,. differentiation, and DNA damage. Genes Dev. 9, -
PII: S1388-1981(99)00150-X
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/10570253.pdf20 Apr 2002: Review. Mammalian phosphoinositide-speci¢c phospholipase C. Roger L. Williams Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK. Received 1 April 1999 ; received in revised form 17 June 1999 ; accepted 31
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