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3 Feb 1997: 8. C, and 200. m. l fractions were collected. 13 ml Sucrose Gradients. ... d. ). Thispellet was washed twice with 200. m. l of the above nondetergent buffer toremove traces of detergent, and then resuspended.

10 Oct 1997: Interestingly, although P2 (200 mM)efficiently blocks the Amph1– dynamin interaction, thebinding of Amph2 SH3 domain is much less wellinhibited (Figure 4B). ... GST-tagged SH3 domains were incubated as in A but in thepresence or absence of each of the

9 Jan 1998: H3 S5 H4 S6 HL2. TGTGSAPVVAEISKKIGALTVAVVTLPFVMEGKVRMKNAMEGLERLKQHTDTLVVIPNEKLFEIVPN140 150 160 170 180 190 200. ... The frozen pellet was powdered under liquid nitrogen and poureddirectly into 200 ml of boiling buffer A (50 mM Tris-HCl, 300 mM NaCl,

15 Jan 1998: 433 440 (Tb4) 1.15 (8)V 173 178 498 503 (Tb5) 3.36 (6)Vb 182 188 VI 193 200 518 524 (Tb6) 1.10 (5)VII 226 235 546

16 Jul 1998: 200 ml was applied by gravity to amoter in the SP64 plasmid, have been described previously (Simon100 ml column of ConA, previously equilibrated in extraction bufferet al., 1987; Hegde et al., ... the first 200 ml of the eluate were savedhistidine

9 Sep 1998: The EMBO Journal Vol.17 No.18 pp.5273–5285, 1998. Crystal structure of the amphiphysin-2 SH3 domainand its role in the prevention of dynamin ringformation. D.J.Owen, P.Wigge, Y.Vallis, J.D.A.Moore,P.R.Evans and H.T.McMahon1. MRC Laboratory of

18 Feb 1999: 110 120 130 140 150 160 170 180 190 200. 210 220 230 240 250 260 270 280 290 300.

3 Mar 1999: continued >. Subject 200: MacroeconomicsCourse Co-ordinator: Mr K. J. Coutts. MR K.

3 Mar 1999: DR P. J. FORDIntroduction to Part I of the Tripos (One lecture,. 6 Oct.). Tu. 2 Lady Mitchell HallDR E. ESCHMML Learning Day. S. 2–4 (One lecture, 10 Oct.). Surnames A–M 2–3Surnames N–Z 3–4. C L A S S I C A L G R E E K A N D L AT I

3 Mar 1999: SPECIAL NO. 2] LECTURE-LIST–MICHAELMAS TERM 1998 169. NAT U R A L S C I E N C E S T R I P O S, PA RT I A. M I C H A E L M A S 1 9 9 8 L E N T 1 9 9 9 E A S T E R 1 9 9 9. L E A R N I N G DAY. Committee for the Natural Sciences Tripos Learning Day

20 Apr 1999: Twenty four micrographs out of 200 in the tilt range of 0–60 wereselected and scanned on a Zeiss SCAI scanner with a 28µm step size.Images were processed with the

30 Apr 1999: 2,000. 500. a. Time (min). Time (min). 200. µM GTP. GT. ... ferase-tagged amphiphysin-2 SH3 domain on glutathione–agarose beads at 4 C. After extensive washing of the matrix in buffer A (200 mM NaCl, 20 mM HEPES, pH 7.3,

6 May 1999: Thus anunfolding of the hinge sub-domain may expose theTolA-binding site on D1 and release the g3pdomains like beads on a string, allowing them tospan a large distance (> 200 AÊ )

8 May 1999: O R D E R S O F E X A M I N AT I O N S. Archaeology and Anthropology. 2Architecture. 3Anglo-Saxon, Norse, and Celtic. 4Chemical Engineering. 5Classics. 6Computer Science. 7Economics. 8Education. 9Engineering. 11Electrical and Information Sciences.

26 May 1999: 99(. est). 1999. –200. 0(es. t). Research Grants and Contracts. Other Income. ... est). 1999. –200. 0(es. t). Purc. hasi. ng P. ower. at 1.

26 May 1999: 50). 0 (3. ,650. )(3. ,200. )0. (3,2. 00). (3,2. 96). ... es0. 1,10. 0 1,. 100. 0 1,. 200. 1,20. 0 0.

10 Sep 1999: mukB V.cholmukB H.inflmukB Y.pestmukB E.coli. S6 H4 H5 H6. ... Protein was eluted with the same buffercontaining 400 mM imidazole. The protein was applied to a sephacrylS-200 column (Amersham-Pharmacia) equilibrated in 20 mM Tris,200 mM NaCl, 1 mM

8 Oct 1999: Th. F. 4, Th. 9. Subject 200: MacroeconomicsCourse Co-ordinator: Mr S.

8 Oct 1999: 200 LECTURE-LIST–MICHAELMAS TERM 1999 [SPECIAL NO. 2. DR R. A. FOLEY AND DR S.

8 Oct 1999: DR D. W. HOLTON AND OTHERSIntroduction to Part I of the Tripos. Tu. 2–3 (One. lecture, 5 Oct.) Lady Mitchell HallMR G. BURNAGEIntroduction to Computer-Assisted Language. Learning. Tu. 3–4, 4–51 (One lecture, 5 Oct.) Lady Mitchell Hall. MR A.

11 Nov 1999: Ras binding domain Nα1 Nα2 Nα3 Nα4 Rβ1 Rβ2 Rα1. 150 160 170 180 190 200 210 220 230 240 250. ... contain one protein molecule in the asymmetric unit.Diffraction data were collected at ESRF beamlines ID2 and ID14-4 at 100K after freezingcrystals in

9 Dec 1999: sym. pto. mat. ic. Age (days)200 400 600 800. c. b(A. ... 80. 60. 40. 20. 00 100 200 300 400. (A117V) H(STE).

18 Jan 2000: COMPULSORY CORE COURSESSubject 200: Macroeconomics. p. 87 PROF. W. H. BUITERMacroeconomics.

25 Apr 2000: 200–650 (MLT1 Paper ML1)O11. Classical traditions in the sciences (NST2HP Paper 1) Wesley Church,.

14 Jun 2000: Sp. ecia. l ini. tiat. ives. 01,. 200. 1,20. 00. 5,35. ... 95,. 359. 02,. 000. 2,00. 00. 2,20. 02,. 200. 02,. 400.

31 Jul 2000: 0 200 400 600 800 1,000 1,200 1,400 1,600 1,800. 1989. ... TOTAL FOR ALL 200 122 80 60 157 87 437 269 126 95 90 58 208 224 424 377COLLEGES.

18 Aug 2000: 2,200 19.2 11,4731975–76 7,127 80.4 1,732 19.6 8,859 2,070 79.5 534 20.5 2,604 9,197 80.2 ... 64.7 3,353 35.3 9,503 2,050 72.2 789 27.8 2,839 8,200 66.4 4,142 33.6 12,3421985–86 6,232

13 Sep 2000: Figure 2D) as published earlier (Löwe and Amos, 1999).Tubes assembled in the presence of GMPCPP show re-peats of 200 Å, 100 Å, 67 Å, and 50 Å (Figure 2E, ... In the case of the GMPCPP-inducedtubes, groups producing the 200 Å longitudinal

29 Sep 2000: 12, F. 2, 9. Subject 200: MacroeconomicsCourse Co-ordinator: Mr K. Coutts.

29 Sep 2000: DR D. W. HOLTON AND OTHERSIntroduction to Part I of the Tripos. Tu. 2–3. (One lecture, 3 Oct.) Lady Mitchell HallMR G. BURNAGEIntroduction to Computer-Assisted Language Learning. Tu. 3–4, 4–51 (One lecture, 3 Oct.) Lady Mitchell HallMS N.

29 Sep 2000: SPECIAL NO. 1] LECTURE-LIST–MICHAELMAS TERM 2000 169. DR S. H. P. MADDRELLThe Living Cell. (Four lectures). PROF. D. J. ELLARMacromolecules in the Cell. (Five lectures). DR J. DAVIESMembranes: Molecular Superstructure. (Five lectures). DR K. V.

10 Nov 2000: 200,000 3 g for 40 min.

1 Dec 2000: The displacement experiment was carried out by mixing 1 mMcontaining 1mM EDTA and 1mM DTT before addition into the assays.PI3K and 2 mM mant-ATP with 50 or 200 mM ... Equilibriumassay with a final ATP concentration of 200 mM with 1.26 mM MgCl2,and

19 Jan 2001: Bore £320.00Squash – Men’s £1,300.00Squash – Women’s Joint ApplicationSwimming and Waterpolo £6,200.00Table Tennis £320.00Trampoline £550.00 £1,000.00Volleyball £1,900.00 £957.50TOTAL

20 Feb 2001: The protein is highlysoluble and stable in 200 mM sodium chloride-containing buffer, and runs as a monomer underthese conditions on size-exclusion columns (datanot shown). ... 20 % (w/v) glycerol,200 mM NaCl, and 0.004 % (v/v) DMSO.

5 Mar 2001: The protein was loaded directly onto a Sephacryl S200column (Amersham-Pharmacia) equilibrated in 200 mM NaCl, 20mM Tris, 1 mM EDTA and 1 mM NaN3 pH 7.0.

6 Mar 2001: For the continuousenzyme-coupled assay, purine nucleotide phosphorylase (20 U ml-1) and 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG) (200 mM) were also present in thereaction mix from the

4 May 2001: 200 column buffer. ... solution, andadditionally 200 mM NaI in the drop.

24 May 2001: Continuity and change in Latin literature, from 200–650 (MLT1 Paper. ML1) Sidgwick Avenue Lecture-roomsWednesday 30 May 9–12 A2.

19 Jun 2001: Purchasing power of University expenditure 1992Ð2002. 0.0. 50.0. 100.0. 150.0. 200.0. ... com. e8,. 289. 15,2. 0023. ,489. 8,81. 115. ,200. 24,0. 118,.

4 Jul 2001: 0 200 400 600 800 1,000 1,200 1,400 1,600 1,800 2,000. ... 38 27 122 86Wolfson College 70 47 48 26 111 62 229 135 65 43 43 22 92 48 200 113.

20 Aug 2001: 2,200 19.2 11,4731975–76 7,127 80.4 1,732 19.6 8,859 2,070 79.5 534 20.5 2,604 9,197 80.2 ... 64.7 3,353 35.3 9,503 2,050 72.2 789 27.8 2,839 8,200 66.4 4,142 33.6 12,3421985–86 6,232

30 Aug 2001: S8 H4 S9 S10 S11 H5. 200 210 220 230 240 250ER.GYSFSTTAEREIVRDIKEKLCYVALDFEQEMQTAAQSSSIEKSYELP.DGQVITIG.NKTYNLMI.GDRTAEAIKMEIGSAE.APEESD.NMEIRGRDLLTGLPKTIEITGKETYRVAI.GERTAERVKIEIGNVF.PSKEND.ELETTVSGIDLSTGLPRKLTLKGG. ... 200 210 220 230 240. H6 H7 S12 S13

28 Sep 2001: Subject 200: MacroeconomicsCourse Co-ordinator: Mr K. Coutts. MR K. COUTTS, DR G.

28 Sep 2001: 200). T WO PA P E R S U B J E C T SCellular and Molecular Pharmacology.

28 Sep 2001: DR S. H. P. MADDRELLThe Living Cell. (Four lectures). PROF. D. J. ELLARMacromolecules in the Cell. (Five lectures). DR J. DAVIESMembranes: Molecular Superstructure. (Five lectures). DR K. JOHNSTONE AND DR K. V. BRINDLEEnergy and Biosynthesis. (Ten

3 Nov 2001: In fungi b-tubulin alsohas a phenylalanine at position 202, and accord-ingly residue 167 is an alanine while there is noconsensus for position 200.

10 Apr 2002: Membership Members Male Female. Student 2166 1281 885Staff 299 200 99Family 35 10 25UCLES/CUP 16 12 4Alumni (concessions) 18 15 3Alumni 6 5 1TOTAL 2540 1523 1017.

19 Apr 2002: Insect cell extracts containing defined concentrations of Smc1 as the ligand (five dilutions, ranging from 20nM to 200 nM) were floated over the bound analytes, and association and dissociation kinetics were

20 Apr 2002: 21 ions. Enzyme(200 ng) was added to the subphase, via an injection port in the Teflontrough, 5 min after the monolayer was spread to allow the surfacepressure to stabilize. ... Replacement of these residues byalanine resulted in a great reduction of the