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Calcium-dependent Membrane Penetration Is a Hallmark of theC2 Domain…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9668093.pdf20 Apr 2002: THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 273, No. 30, Issue of July 24, pp. -
Catalytic Domain of Phosphoinositide-specificPhospholipase C…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9565585.pdf20 Apr 2002: PLC Activity in Mixed Micelles—The assay of hydrolysis of PIP2 andPI was based on methods described previously (19, 21, 24, 25). ... Thecalcium dependence of PIP2, PIP, and PI hydrolysis was analyzed usinga range of calcium buffers as described -
Crystal Structure of a Calcium-Phospholipid Binding Domainfrom…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9430701.pdf20 Apr 2002: Crystal Structure of a Calcium-Phospholipid Binding Domainfrom Cytosolic Phospholipase A2. (Received for publication, September 24, 1997). ... 1991) J. Appl. Crystallogr. 24, 946 –95072. Nicholls, A. (1992) GRASP: Graphical Representation and Analysis -
Phosphoinositide-Specific Phospholipase Cδ1 Activity toward Micellar…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9287165.pdf20 Apr 2002: diC7PC (8 mM) no significant activation observed 0.85( 0.06 24.7( 2.9(1.7( 0.2). ... 132) PI-PLCδ1 and at 24-45 C for full-length PI-PLCδ1 (Figure 7A,B). -
Proc. Natl. Acad. Sci. USAVol. 94, pp. 9637–9642, September ...
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9275175.pdf20 Apr 2002: An initial model wasbuilt using the program O (24). Noncrystallographic symmetryrestrained positional and temperature factor refinement werecarried out with the program X-PLOR. ... S. (1993) Acta Crystallogr. D 49,. 129 –147.24. Jones, T. A., Zou, J.-Y. -
A Ternary Metal Binding Site in the C2 Domain ...
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9062102.pdf20 Apr 2002: 1996)J.Biochem. 120, 671-676. Kraulis, P. J. (1991)J. Appl. Crystallogr. 24, 946-950.Lee, S. -
Structural Mapping of the Catalytic Mechanism for a…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9048554.pdf20 Apr 2002: 4.6)〈I/σ(I)〉b 24.1(5.1) 15.7(4.3) 20.6(4.4) 17.2(4.9). -
cdf519 5057..5068
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/12356722.pdf12 Oct 2002: Resolution (AÊ ) 2.0 2.0Observations/unique reections 83 922/24 307 84 280/24 356Completeness (last shell) % 93.6 (74.1) 93.7 (74.1)Rmerge. ... The supernatant was collected anddialysed against 50 mM TrisHCl pH 8.0 and 1.5 M urea for 16 h, andthen -
PII: S1097-2765(01)00372-0
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/11684018.pdf20 Apr 2002: Negatively charged regions are shown in red and positively chargedin blue (ranging from 18 kT/e to 24 kT/e). ... Crystallogr.heavy-atom parameter refinement for multiple isomorphous re- 24, 946–950.placement and multiwavelength anomalous diffraction -
Tyrosine Residues in Phospholipase C�2 Essential for the…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/11606584.pdf20 Apr 2002: baculovirus expression of Src family kinases were madeby subcloning the cDNAs (24) encoding activated Src (Y527F Src),activated Fyn (Y531F Fyn), and activated Lck (Y525F Lck) into thebaculovirus transfer vector ... Some of the additional sites couldbe
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